Cell therapy and biological wound coatings in the treatment of patients with skin defects

Bogdanov S.B.; Karakulev A.V.; Poliakov A.V.; Alad’ina V.A.; Gilevich I.V.; Melkonyan K.I.; Sotnichenko A.S.

doi:https://doi.org/10.17116/plast.hirurgia201904143

Bogdanov S.B.

Scientific Research Institution — Ochapovsky Regional Clinical Hospital #1, Krasnodar, Russia;
Kuban State Medical University, Krasnodar, Russia

Karakulev A.V.

Scientific Research Institution — Ochapovsky Regional Clinical Hospital #1, Krasnodar, Russia;
Kuban State Medical University, Krasnodar, Russia

Poliakov A.V.

Mediko-geneticheskiĭ nauchnyĭ tsentr RAMN, Moskva

Alad’ina V.A.

Kuban State Medical University, Krasnodar, Russia

Gilevich I.V.

Scientific Research Institution — Ochapovsky Regional Clinical Hospital #1, Krasnodar, Russia;
Kuban State Medical University, Krasnodar, Russia

Melkonyan K.I.

Kuban State Medical University, Krasnodar, Russia

Sotnichenko A.S.

Kuban State Medical University, Ministry of Health of the Russian Federation, Krasnodar, Russia

Cell therapy and biological wound coatings in the treatment of patients with skin defects

Authors:

Bogdanov S.B., Karakulev A.V., Poliakov A.V., Alad’ina V.A., Gilevich I.V., Melkonyan K.I., Sotnichenko A.S.

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Journal: Plastic Surgery and Aesthetic Medicine. 2019;(4): 43‑49

DOI: 10.17116/plast.hirurgia201904143

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To cite this article:

Bogdanov SB, Karakulev AV, Poliakov AV, Alad’ina VA, Gilevich IV, Melkonyan KI, Sotnichenko AS. Cell therapy and biological wound coatings in the treatment of patients with skin defects. Plastic Surgery and Aesthetic Medicine. 2019;(4):43‑49. (In Russ.)
https://doi.org/10.17116/plast.hirurgia201904143

Подписка 2026 Пластическая хирургия и эстетическая медицина (Plastic Surgery and Aesthetic Medicine)

Использование инфографики авторами научных работ

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Abstract:

Severe thermal trauma is still life-threatening disease with high incidence of severe complications despite significant progress in the treatment of these patients. Standard treatment strategy is surgical excision of necrotic skin followed by autodermoplasty. However, extensive and deep burn wounds may be associated with donor skin deficit. Therefore, cell therapy may be required. This review is devoted to development of cell therapy, historical aspects and current trends in the treatment of patients with thermal trauma using cell technology. The authors presented a new experimental method of wound formation for analysis of biological wound coatings and cell therapy.

Keywords:

burns

combustiology

cell therapy

fibroblasts

keratinocytes

experimental model

Authors:

Bogdanov S.B.

Scientific Research Institution — Ochapovsky Regional Clinical Hospital #1, Krasnodar, Russia;
Kuban State Medical University, Krasnodar, Russia

Karakulev A.V.

Scientific Research Institution — Ochapovsky Regional Clinical Hospital #1, Krasnodar, Russia;
Kuban State Medical University, Krasnodar, Russia

Poliakov A.V.

Mediko-geneticheskiĭ nauchnyĭ tsentr RAMN, Moskva

Alad’ina V.A.

Kuban State Medical University, Krasnodar, Russia

Gilevich I.V.

Scientific Research Institution — Ochapovsky Regional Clinical Hospital #1, Krasnodar, Russia;
Kuban State Medical University, Krasnodar, Russia

Melkonyan K.I.

Kuban State Medical University, Krasnodar, Russia

Sotnichenko A.S.

Kuban State Medical University, Ministry of Health of the Russian Federation, Krasnodar, Russia

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References:

Vinnik YuS, Salmina AB, Drobushevskaya AI, Teplyakova OV, Pozhilenkova EA, Zykova LD. Kletochnie technologii i tkanevaya ingeneriya v lechenii dlitel’no ne zagivauchih ran. Vestnik eksperimental’noy i clinicheskoy khirurgii. 2011;4(2):392-397. (In Russ.)
Brusselaers N, Pirayesh A, Hoeksema H, Richters CD, Verbelen J, Beele H, Blot SI, Monstrey S. Skin replacement in burn wounds. Journal of Trauma and Acute Care Surgery. 2010;68(2):490-501. https://doi.org/10.1097/TA.0b013e3181c9c074
Groeber F, Holeiter M, Hampel M, Hinderer S, Schenke-Layland K. Skin tissue engineering – in vivo and in vitro applications. Advanced drug delivery reviews. 2011;63(4-5):352-366. https://doi.org/10.1016/j.cps.2011.09.007
Leclerc T, Thepenier C, Jault P, Bey E, Peltzer J, Trouillas M, Duhamel P, Bargues L, Prat M, Bonderriter M, Lataillade JJ. Cell therapy of burns. Cell proliferation. 2011;44:48-54. https://doi.org/10.1111/j.1365-2184.2010.00727.x
Dimitropoulos G, Jafari P, de Buys Roessingh A, Hirt-Burri N, Raffoul W, Applegate LA. Burn patient care lost in good manufacturing practices? Annals of burns and fire disasters. 2016;29(2):111-115.
Panov AV, Shapovalov SG, Pleshkov AS, Spichkina OG, Kalmykova NV. Pervyj opyt primeneniya cellyulyarnomatrichnogo kompleksa. Sbornik nauchnyh trudov «IV s”ezd kombustiologov». Moskva: Ob”edinenie kombustiologov «Mir bez ozhogov»; 2013. (In Russ.)
Nanchahal J, Dover R, Otto W, Dhital SK. Cultured composite skin grafts: biological skin equivalents permitting massive expansion. The Lancet. 1989;334(8656):191-193. https://doi.org/10.1136/bmj.301.6765.1342
Zorin VL, Zorina AI, Petrakova OS, Cherkasov VR. Dermal’nye fibroblasty dlya lecheniya defektov kozhi. Geny i kletki. 2009;4(4):26-40. (In Russ.)
Tumanov VP. Sovremennye kletochnye tekhnologii. Novosti klinicheskoy tsitologii Rossii. 2007;1:34-38. (In Russ.)
Chua AW, Khoo YC, Tan BK, Tan KC, Foo CL, Chong SJ. Skin tissue engineering advances in severe burns: review and therapeutic applications. Burns & trauma. 2016;4(1):3. https://doi.org/10.1186/s41038-016-0027-y
Alekseev AA, Salahiddinov KZ, Gavrilyuk BK, Tyurnikov YuI. Kompleksnoe lechenie glubokih ozhogov na osnove primeneniya hirurgicheskoj nekrektomii i sovremennyh biotekhnologicheskih metodov. Annaly hirurgii. 2012;6:41-45. (In Russ.)
Sarkisov DS, Tumanov VP, Glushchenko EV, Morozov SS. Ispol’zovanie kul’tivirovannyh fibroblastov pri lechenii obozhzhennyh. Byulleten’ eksperimental’noj biologii i mediciny. 1990;3:400-402. (In Russ.)
Kolokol’tsova TD, Yurchenko ND, Kolosov NG, Shumakova OV, Nechaeva EA. Perspektivy ispol’zovaniya fetal’nyh fibroblastov cheloveka pri lechenii ran razlichnoj etiologii. Vestnik RAMN. 1998;3:32-35. (In Russ)
Sarkisov DS, Alekseev AA, Glushchenko EV. Teoreticheskie i prakticheskie aspekty ispol’zovaniya kul’tivirovannyh fibroblastov pri vosstanovlenii celostnosti kozhnogo pokrova. Vestnik RAMN. 1994;7:6-11. (In Russ.)
Cuono C, Langdon R, McGuire J. Use of cultured epidermal autografts and dermal allografts as skin replacement after burn injury. The Lancet. 1986;327(8490):1123-1124. https://doi.org/10.1016/S0140-6736(86)91838-6
Cuono CB, Langdon R, Birchall N, Barttelbort S, McGuire J. Composite autologous-allogeneic skin replacement: development and clinical application. Plastic and Reconstructive Surgery. 1987;80(4):626-637. https://doi.org/10.1097/00006534-198710000-00029
Hansbrough JF, Franco ES. Skin replacements. Clinics in plastic surgery. 1998;25(3):407-423.
Shahrokhi S, Arno A, Jeschke MG. The use of dermal substitutes in burn surgery: acute phase. Wound Repair and Regeneration. 2014;22(1):14-22. https://doi.org/10.1111/wrr.12119
Zorina AI, Bozo IYa, Zorin VL, Cherkasov VR, Deev RV. Fibroblasty dermy: osobennosti citogeneza, citofiziologii i vozmozhnosti klinicheskogo primeneniya. Geny i kletki. 2011;6(2):15-26. (In Russ.)
Philandrianos C, Andrac-Meyer L, Mordon S, Feuerstein JM, Sabatier F, Veran J, Magalon G, Casanova D. Comparison of five dermal substitutes in full-thickness skin wound healing in a porcine model. Burns. 2012;38(6): 820–829. https://doi.org/10.1016/j.burns.2012.02.008
Aleynik DYa, Zorin VL, Eremin II, Korsakov IN, Charykova IN. Ispol’zovanie kletochnyh tekhnologij dlya vosstanovleniya povrezhdenij kozhi pri ozhogovoj travme. Sovremennye problemy nauki i obrazovaniya. 2015;4:331. (In Russ.)
Conconi MT, De Coppi P, Di Liddo R, Vigolo S, Zanon GF, Parnigotto PP, Nussdorfer GG. Tracheal matrices, obtained by a detergent-enzymatic method, support in vitro the adhesion of chondrocytes and tracheal epithelial cells. Transplant international. 2005;18(6):727-734.
Burra P, Tomat S, Conconi MT, Macchi C, Russo FP, Parnigotto PP, Naccarato R, Nussdorfer GG. Acellular liver matrix improves the survival and functions of isolated rat hepatocytes cultured in vitro. International journal of molecular medicine. 2004;14(4):511-516.
Gallico GG 3rd, O’Connor NE, Compton CC, Kehinde O, Green H. Permanent coverage of large burn wounds with autologous cultured human epithelium. New England Journal of Meditsine. 1984;311(7):448-451.
van der Veen VC, van der Wal MB, van Leeuwen MC, Ulrich MM, Middelkoop E. Biological background of dermal substitutes. Burns. 2010;36(3):305-321.
Climov M, Medeiros E, Farkash EA, Qiao J, Rousseau CF, Dong S, Zawadzka A, Racki WJ, Al-Musa A, Sachs DH, Randolph MA, Huang CA, Bollenbach TJ. Bioengineered self-assembled skin as an alternative to skin grafts. Plastic and Reconstructive Surgery Global Open. 2016;4(6):e731.
MacNeil S. Progress and opportunities for tissue-engineered skin. Nature. 2007;445(7130):874-880.
Boyce ST, Kagan RJ, Yakuboff KP, Meyer NA, Rieman MT, Greenhalgh DG, Warden GD. Cultured skin substitutes reduce requirements for harvesting of skin autograft for closure of excised, full-thickness burns. J Trauma. 2006;60(4):821-829.
Golinski P, Menke H, Hofmann M, Valesky E, Butting M, Kippenberger S, Bereiter-Hahn J, Bernd A, Kaufmann R, Zoeller NN. Development and characterization of an engraftable tissue-cultured skin autograft: alternative treatment for severe electrical injuries. Cells Tissues Organs. 2014;200(3-4):227-239.
Zöller N, Valesky E, Butting M, Hofmann M, Kippenberger S, Bereiter-Hahn J, Bernd A, Kaufmann R. Clinical application of a tissue-cultured skin autograft: an alternative for the treatment of non-healing or slowly healing wounds? Dermatology. 2014;229(3):190-198.
Tkachuk VA. Autologichnye stvolovye kletki: eksperimental’noe issledovanie i perpektivy klinicheskogo primeneniya. Rukovodstvo dlya vrachey. Pod red. Tkachuk VA. Moskva: Litterra, 2009. (In Russ.)
Porhanov VA, Bogdanov SB, Gilevich IV, Fedorenko TV, Kolomijceva EA, Bogdanova YuA. Novye podhody k vypolneniyu plastiki kozhi lica polnoslojnymi autotransplantatami. Ortopediya, travmatologiya i vosstanovitel’naya hirurgiya detskogo vozrasta. 2017;5(4):68-73. (In Russ.) https://doi.org/10.17816/PTORS5468-73
Bogdanov SB, Porhanov VA, Gilevich IV, Fedorenko TV, Kolomijceva EA, Polyakov AV. Vozmozhnosti primeneniya kletochnoj terapii v kozhnoplasticheskih operaciyah. Innovacionnaya medicina Kubani. 2018;3(11):16-22. (In Russ.)
Bogdanov SB, Porhanov VA, Polyakov AV, Vinogradov R, Dikarev AS, Zabunyan GA, Bogdanova YuA, Gilevich IV, Matyuha SA. Sovershenstvovanie hirurgicheskih tekhnologij plastiki bol’shim autosal’nikom dlya lecheniya defektov volosistoj chasti golovy i lica. Annaly plasticheskoj, rekonstruktivnoj i esteticheskoj hirurgii. 2018;2:10-17. (In Russ.)
Bogdanov SB, Porhanov VA, Polyakov AV, Vinogradov RA, Dikarev AS, Zabunyan GA, Bogdanova YuA, Matyuha SA, Gilevich IV. Patent «Sposob kozhnoj autoplastiki». №2657202 on 08.06.18. Byul. №16. (In Russ.)
Bogdanov SB, Porhanov VA, Gilevich IV, Fedorenko TV, Kolomijceva EA. Patent «Sposob plastiki polnoslojnym kozhnym autotransplantatom». Patent №2655201. Vypusk ot 24.05.18. Byul. №15. (In Russ.)
Wu SC, Marston W, Armstrong DG. Wound care: the role of advanced wound healing technologies. Journal of vascular surgery. 2010;52(3):59S-66S.
Sha H, Fu X. Naturally derived materials-based cell and drug delivery systems in skin regeneration. Journal of Controlled Release. 2010;142 (2):149-159.