OBJECTIVE
To determine intestinal tissues healing properties based on it’s membrane-stabilizing properties, in acute intestinal obstruction.
MATERIAL AND METHODS
A clinical and laboratory studies of 24 patients with acute small bowel obstruction developed against the background of peritoneal commissures, inversion, closed-loop, strangulated hernia was performed. Surgical interventions were accompanied by resection of the small intestine within the regulated limits (40 cm in the adductor’ and 20 cm in the diverting’ segments). The tissues of the resected adductor segment of the small intestine at a distance of 20 cm (first level) and 40 cm (second level) were studied of lipid metabolism. Membrane tissues’ lipid peroxidation activity was studied and measured by the content of primary and secondary molecular products: by superoxide dismutase activity of — antioxidant enzyme potential, by phospholipase A2 activity — phospholipase potential. The qualitative and quantitative lipid composition of intestinal tissues was determined by extraction of lipids, separation and molecular analysis.
RESULTS
Data analysis from intestinal tissues lipid metabolism in patients with acute intestinal obstruction showed significant disturbances of metabolism occur in the resected part of the organ. At distance of 40 cm from barrier significant deviations of qualitative and quantitative composition were recorded. It has been shown that lipid metabolism disorders are associated with a pronounced intensification of peroxidation of membrane lipids and phospholipases in intestinal tissue structures. Decrease in the activity of trigger agents was registered away from the area of obstruction (barrier). The discovered fact of the presence of pronounced membrane-stabilizing phenomena in this department also indicates possible failures of reparations.
CONCLUSION
The obtained data proves that in acute intestinal obstruction, a decrease in the reparative potential of tissues is possible even 40 cm from the obstruction. In order to increase the reliability of intestinal anastomosis in these conditions, it is necessary to use the most adequate operational technologies and drug regimens, especially with smaller volumes of resection.
