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Dentovskaya S.V.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Platonov M.E.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Shaikhutdinova R.Z.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Svetoch T.E.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Ivanov S.A.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Gapel’chenkova T.V.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Krasil’nikova E.A.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Trunyakova A.S.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Kombarova T.I.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Vagaiskaya A.S.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Lipatnikova N.A.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Mazurina E.M.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Kolombet L.V.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Anisimov A.P.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

SurA is required for outer membrane biogenesis and can be used as a new molecular target for plague therapy

Authors:

Dentovskaya S.V., Platonov M.E., Shaikhutdinova R.Z., Svetoch T.E., Ivanov S.A., Gapel’chenkova T.V., Krasil’nikova E.A., Trunyakova A.S., Kombarova T.I., Vagaiskaya A.S., Lipatnikova N.A., Mazurina E.M., Kolombet L.V., Anisimov A.P.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2024;42(2): 3‑9

DOI: 10.17116/molgen2024420213

Read: 704 times

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Table of contents

SURA IS REQUIRED FOR OUTER MEMBRANE BIOGENESIS AND CAN BE USED AS A NEW MOLECULAR TARGET FOR PLAGUE THERAPY
SURA IS REQUIRED FOR OUTER MEMBRANE BIOGENESIS AND CAN BE USED AS A NEW MOLECULAR TARGET FOR PLAGUE THERAPY

To cite this article:

Dentovskaya SV, Platonov ME, Shaikhutdinova RZ, et al. . SurA is required for outer membrane biogenesis and can be used as a new molecular target for plague therapy. Molecular Genetics, Microbiology and Virology. 2024;42(2):3‑9. (In Russ.)
https://doi.org/10.17116/molgen2024420213

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
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Recommended articles:
Disruption of outer membrane biogenesis leads to atte­nuation of Yersinia pseudotuberculosis. Mole­cular Gene­tics, Microbiology and Viro­logy. 2025;(2):29-34
Effi­ciency enha­ncement of a candidate plague vaccine by inclusion into its composition of two strains, atte­nuated at different mole­cular targets. Mole­cular Gene­tics, Microbiology and Viro­logy. 2025;(2):35-42
Abstract:

Goal. Determination of the role of periplasmic chaperone SurA in the pathogenesis of bubonic and pneumonic plague.

MATERIALS AND METHODS

Yersinia pestis surA knockout mutants were generated by RedGam mutagenesis with the use of a suicide vector. The generated strains were characterized by their growth rate, sensitivity to antibiotics, bile acid salts and sodium dodecyl sulfate, as well as to the bactericidal action of normal human serum. Intracellular localization of the SurA protein was determined by DSN-PAAG and immunoblot methods. The virulence of the ΔsurA mutant was studied in subcutaneously or intranasally infected mice or rats.

RESULTS

The SurA-negative Y. pestis strain was characterized by an increase in the permeability of the outer membrane and sensitivity to antibiotics, as well as a decrease in virulence in a model of bubonic and pneumonic plague in two laboratory animal species.

CONCLUSION

The SurA protein can be considered as a molecular target for plague therapy. Reducing the virulence of a strain and its resistance to antibiotics by disrupting the biogenesis of outer membrane proteins may become a new strategy for solving the problem of combating multidrug resistance, and the widespread distribution of the protein among Gram-negative pathogens makes its use promising for the development of virulence inhibitors with a broad spectrum of action.

Keywords:

virulence inhibitors
outer membrane
chaperones
Yersinia pestis

Authors:

Dentovskaya S.V.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Platonov M.E.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Shaikhutdinova R.Z.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Svetoch T.E.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Ivanov S.A.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Gapel’chenkova T.V.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Krasil’nikova E.A.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Trunyakova A.S.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Kombarova T.I.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Vagaiskaya A.S.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Lipatnikova N.A.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Mazurina E.M.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Kolombet L.V.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Anisimov A.P.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Received:

08.01.2024

Accepted:

15.02.2024

Close metadata

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