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Trunyakova A.S.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Shaikhutdinova R.Z.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Gapel’chenkova T.V.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Vagaiskaya A.S.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Lipatnikova N.A.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Krasil’nikova E.A.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Mazurina E.M.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Kombarova T.I.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Dentovskaya S.V.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Anisimov A.P.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Disruption of outer membrane biogenesis leads to attenuation of Yersinia pseudotuberculosis

Authors:

Trunyakova A.S., Shaikhutdinova R.Z., Gapel’chenkova T.V., Vagaiskaya A.S., Lipatnikova N.A., Krasil’nikova E.A., Mazurina E.M., Kombarova T.I., Dentovskaya S.V., Anisimov A.P.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2025;43(2): 29‑34

DOI: 10.17116/molgen20254302129

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Table of contents

DISRUPTION OF OUTER MEMBRANE BIOGENESIS LEADS TO ATTENUATION OF YERSINIA PSEUDOTUBERCULOSIS
DISRUPTION OF OUTER MEMBRANE BIOGENESIS LEADS TO ATTENUATION OF YERSINIA PSEUDOTUBERCULOSIS

To cite this article:

Trunyakova AS, Shaikhutdinova RZ, Gapel’chenkova TV, et al. . Disruption of outer membrane biogenesis leads to attenuation of Yersinia pseudotuberculosis. Molecular Genetics, Microbiology and Virology. 2025;43(2):29‑34. (In Russ.)
https://doi.org/10.17116/molgen20254302129

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
Abstract:

THE GOAL

Of this study is to find out the effect of deletion of some genes encoding biogenesis of components of the outer membrane protein complex on the virulence of Yersinia pseudotuberculosis mutants.

MATERIALS AND METHODS

Y. pseudotuberculosis surA, degP, bamB, and ompH mutants were generated by RedGam mutagenesis using suicide vectors. The constructed mutants were characterized by growth rate, sensitivity to antibiotics, bile salts, and sodium dodecyl sulfate, as well as to the bactericidal action of normal human serum. Total composition of the outer membrane proteins of the knockout mutants was determined by SDS-PAGE. The virulence of DsurA, DdegP, DbamB and DompH was studied after subcutaneous, intraperitoneal, or oral infection of mice.

RESULTS

It was found that BamB, OmpH and SurA proteins were necessary for maintaining the integrity of the Y. pseudotuberculosis cell membrane, SurA and BamB proteins are important for resistance to the bactericidal action of serum complement and a high degree of attenuation of Y. pseudotuberculosis strains with deleted surA, degP, bamB genes for outbred mice was revealed.

CONCLUSION

The surA, degP, and bamB genes can be used as potential molecular targets for construction of Y. pseudotuberculosis vaccine strains.

Keywords:

virulence inhibitors
outer membrane
chaperones
Yersinia pseudotuberculosis

Authors:

Trunyakova A.S.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Shaikhutdinova R.Z.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Gapel’chenkova T.V.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Vagaiskaya A.S.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Lipatnikova N.A.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Krasil’nikova E.A.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Mazurina E.M.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Kombarova T.I.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Dentovskaya S.V.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Anisimov A.P.

The Federal Budgetary Institution of Science «State Research Center for Applied Microbiology and Biotechnology» of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Received:

14.03.2025

Accepted:

16.04.2025

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