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Solomay T.V.

Interregional Directorate No. 1 of the FMBA of Russia;
FGBNU «I.I. Mechnikov Scientific Research Institute of Vaccines and Serums» of the Ministry of Education and Science of Russia

Malahova M.V.

Petrovsky National Research Center of Surgery

Shitikov E.A.

Federal Research and Clinical Center of Physical-Chemical Medicine

Bespiatykh D.A.

Federal Research and Clinical Center of Physical-Chemical Medicine

Veselovsky V.A.

Federal Research and Clinical Center of Physical-Chemical Medicine

Semenenko T.A.

FGBU «National Research Center of Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya» of the Ministry of Health of Russia;
FGAOU VO «I.M. Sechenov First Moscow State Medical University» of the Ministry of Health of Russia (Sechenov University)

Smirnova D.I.

FGBNU «I.I. Mechnikov Scientific Research Institute of Vaccines and Serums» of the Ministry of Education and Science of Russia

Gracheva A.V.

FGBNU «I.I. Mechnikov Scientific Research Institute of Vaccines and Serums» of the Ministry of Education and Science of Russia

Faizuloev E.B.

FSBSI «I. Mechnikov Research Institute of Vaccines and Sera»

Epstein—Barr virus: evaluation of gp350 and EBNA2 gene variability

Authors:

Solomay T.V., Malahova M.V., Shitikov E.A., Bespiatykh D.A., Veselovsky V.A., Semenenko T.A., Smirnova D.I., Gracheva A.V., Faizuloev E.B.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2022;40(3): 32‑40

DOI: 10.17116/molgen20224003132

Read: 1966 times

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Table of contents

EPSTEIN—BARR VIRUS: EVALUATION OF GP350 AND EBNA2 GENE VARIABILITY
EPSTEIN—BARR VIRUS: EVALUATION OF GP350 AND EBNA2 GENE VARIABILITY

To cite this article:

Solomay TV, Malahova MV, Shitikov EA, et al. . Epstein—Barr virus: evaluation of gp350 and EBNA2 gene variability. Molecular Genetics, Microbiology and Virology. 2022;40(3):32‑40. (In Russ.)
https://doi.org/10.17116/molgen20224003132

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

Approximately 90% of the world’s population is infected with the Epstein-Barr virus (EBV), requiring the development of vaccines. The variability of individual genes may affect the success of the vaccination campaign and will necessitate a dynamic update of the antigenic composition of vaccines.

Aim of the study. Assessment of the variability of EBV gp350 and EBNA2 genes isolated from saliva from dental clinic personnel in the Moscow area.

MATERIAL AND METHODS

Bio-samples were obtained from 105 employees at four dental clinics. For EBV positive samples, the EBNA2 genotype and gp350 sequence were determined. A maximum likelihood phylogenetic tree was constructed using the N-terminal fragment of the gp350 protein. Analysis also included 222 EBV samples from the NCBI database.

RESULTS

The EBNA2 genotype and the complete gp350 gene sequence were determined for 31 DNA samples. Based on phylogenetic analysis of the g350 protein, the Russian virus population has been uniformly distributed along the tree. Meanwhile, 30 samples fell into the Aa clade (A — genotype A for the EBNA2 gene; a — similarity of the gp350 sequence with B95-8 [NC_007605.1]), and one sample belonged to the Bb clade (B — genotype B for EBNA2; b — similarity of gp350 with Jijoye [LN827800.1]). For 30 Russian samples of the Aa genotype, 22 individual profiles and 16 unique mutations were found. Identical gp350 profiles were found amongst staff working in close occupational contact.

CONCLUSION

The identified features indicate the need for further phylogenetic studies of samples collected in Russia to develop and introduce vaccines

Keywords:

Epstein—Barr virus
EBV infection
gp350
EBNA2
phylogenetic analysis

Authors:

Solomay T.V.

Interregional Directorate No. 1 of the FMBA of Russia;
FGBNU «I.I. Mechnikov Scientific Research Institute of Vaccines and Serums» of the Ministry of Education and Science of Russia

Malahova M.V.

Petrovsky National Research Center of Surgery

Shitikov E.A.

Federal Research and Clinical Center of Physical-Chemical Medicine

Bespiatykh D.A.

Federal Research and Clinical Center of Physical-Chemical Medicine

Veselovsky V.A.

Federal Research and Clinical Center of Physical-Chemical Medicine

Semenenko T.A.

FGBU «National Research Center of Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya» of the Ministry of Health of Russia;
FGAOU VO «I.M. Sechenov First Moscow State Medical University» of the Ministry of Health of Russia (Sechenov University)

Smirnova D.I.

FGBNU «I.I. Mechnikov Scientific Research Institute of Vaccines and Serums» of the Ministry of Education and Science of Russia

Gracheva A.V.

FGBNU «I.I. Mechnikov Scientific Research Institute of Vaccines and Serums» of the Ministry of Education and Science of Russia

Faizuloev E.B.

FSBSI «I. Mechnikov Research Institute of Vaccines and Sera»

Received:

04.02.2022

Accepted:

15.04.2022

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