Annually a number of tissue typing laboratories (HLA-laboratories) of Russian Federation, performing examinations of potential donors and recipients of organs and tissues, participate in the implementation of international external professional testing of HLA-typing organized by the European Federation for Immunogenetics (EFI), since the external control program currently do not exist in our country.
THE AIM OF THIS STUDY
Was to analyze the effectiveness of the implementation of external professional testing of HLA-typing by laboratories of Russian Federation from 2017 to 2022.
MATERIALS AND METHODS
During this period, from 10 to 16 laboratories of our country took part in the external professional testing of HLA-typing annually. The control trials were organized by the Department of Transfusion Medicine of the Medical University of Vienna (Austria) performing this function under the auspices of the EFI. For research, 10 DNA samples isolated from nucleated peripheral blood cells of healthy blood donors were provided. According to the standards of the EFI, the typing of HLA genes must be performed by all molecular methods used in the laboratory. Participating laboratories presented the low and (or) high/allelic resolution results.
RESULTS
Our analysis showed that during 2017—2019 11.1—28.6% of Russian laboratories failed to perform the low resolution HLA-typing. From 2020 to 2022, the results of all laboratories were considered satisfactory in accordance with EFI standards. Successful implementation of the high/allelic resolution HLA typing by all laboratories was noted only in 2018. During the remaining five years of observation, the results from 10% to 33.3% of Russian laboratories were not successful according to EFI quality standards.
CONCLUSION
The analysis identified the need for the constant participation of HLA typing laboratories in the external professional testing of HLA-typing, as well as the development and implementation of Russian system of external HLA typing quality control and the organization of continuous education of tissue typing laboratory specialists in our country.
