The site of the Media Sphera Publishers contains materials intended solely for healthcare professionals.
By closing this message, you confirm that you are a certified medical professional or a student of a medical educational institution.

Login
EN
+7 (495) 482-4118
+7 (495) 482-0604
+8 800 250-18-12
  • (toll-free number for subscriptions)
    Mon-Fri from 10 a.m. to 6 p.m.

  • © 1998-2025
+7 (495) 482-43-29
EN
All journals
Journal
Year
Year
Search result: 0

Potekaev N.N.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology, Moscow, Russia

Frigo N.V.

Moscow Sientific and Practical Center of Dermatovenereology and Cosmetology, Moscow, Russia, 119071

Petersen E.V.

Moscow Sientific and Practical Center of Dermatovenereology and Cosmetology, Moscow, Russia, 119071;
Moscow Institute of Physics and Technology, Moscow region, Dolgoprudny, Russia, 141701

Artificial skin: types and applications

Authors:

Potekaev N.N., Frigo N.V., Petersen E.V.

More about the authors

Journal: Russian Journal of Clinical Dermatology and Venereology. 2017;16(6): 7‑15

DOI: 10.17116/klinderma20171667-15

Views: 16787

Downloaded: 418

Download PDF (RU)
Contact the author
Table of contents

ARTIFICIAL SKIN: TYPES AND APPLICATIONS
ARTIFICIAL SKIN: TYPES AND APPLICATIONS

To cite this article:

Potekaev NN, Frigo NV, Petersen EV. Artificial skin: types and applications. Russian Journal of Clinical Dermatology and Venereology. 2017;16(6):7‑15. (In Russ.)
https://doi.org/10.17116/klinderma20171667-15

Подписка 2025-2 (Russian Journal of Clinical Dermatology and Venereology)
 
МСФ на ЯМ
Close metadata
Recommended articles:
Cell technologies as a basis for the deve­lopment of rege­nerative principles for the treatment of lacrimal gland diseases. Russian Annals of Ophthalmology. 2024;(2-2):158-165
Sensitivity of various cell cultures to human respiratory syncytial virus from clinical examination of patient. Mole­cular Gene­tics, Microbiology and Viro­logy. 2024;(2):39-44
Morphological features of biopsy samples of a healing ischemic skin defect after transplantation of dermal fibroblasts into the wound with the support of pulmonary surfactant. Russian Journal of Operative Surgery and Clinical Anatomy. 2024;(4):11-17
Hybrid wound coating in reha­bilitation of severe thermal burns. (An expe­rimental study). Problems of Balneology, Physiotherapy and Exercise Therapy. 2024;(6-2):40-49
Effi­ciency of a new method of allo­plasty with dermal equivalent with cultured allo­fibroblasts and with the support of poly­deoxyribonucleotides of an ischemic skin defect. Russian Journal of Operative Surgery and Clinical Anatomy. 2025;(1):22-28

The article presents literature data concerning the history and prospects of cell technology in the treatment of wounds, burns, and skin defects of various etiology. The review reflects the development stages of the techniques for skin cell culture, transplantation, and preparation of skin equivalents. We describe the types of artificial skin, 2D and 3D models of artificial skin, applied aspects of the use of artificial skin, including dermatology.

Keywords:

synthetic skin
living skin equivalents
cell culture
fibroblasts
keratinocytes
application in dermatology

Authors:

Potekaev N.N.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology, Moscow, Russia

Frigo N.V.

Moscow Sientific and Practical Center of Dermatovenereology and Cosmetology, Moscow, Russia, 119071

Petersen E.V.

Moscow Sientific and Practical Center of Dermatovenereology and Cosmetology, Moscow, Russia, 119071;
Moscow Institute of Physics and Technology, Moscow region, Dolgoprudny, Russia, 141701

List of references:

  1. Khodadadi L et al. Cell therapy in Burn Repair. Yakhteh Med. J. 2008;10(3):167-178.
  2. Koroleva TA. Cell technologies in the treatment of children with deep skin burns (review). Ros vestn. 2013;III(3):35-42. (In Russ.)
  3. Medawar PR. The cultivation of adult mammalian skin epithelium in vitro. Quart J Microsc Sci. 1948;89:187-196.
  4. Gavrilenko AV, Pavlova ОV, Vachratyan PE. The use of fibroblasts and keratinocytes in complex treatment of venous trophic ulcers. Surgery. N.I. Piragov Journal 2008;10:80-83. (In Russ.)
  5. Alekseev AA, Popov SV. Modern methods of transplantation of cultured skin cells and its equivalents in the treatment of burns. Combustiology (electronic journal) 1999. (In Russ.)
  6. Fedorov VD, Sarkisov DS, Alekseev AA, et al. Plastic restoration of skin integuments using cultured allogenic fibroblasts. Annals of Surgery 1996;4:16-19. (In Russ.)
  7. Karasek MA, Charlton ME. Growth of postembryonic skin epithelial cells on collagen gels. J Invest Dermatol. 1971 Mar;56(3):205-210.
  8. Moore JT, Karasek MA. Isolation and properties of a germinative and a non-germinative cell population from postembryonic mouse, rabbit, and human epidermis. J Invest Dermatol. 1971 Apr;56(4):318-324.
  9. Fredriksson C, Kratz G, Huss F. Transplantation of cultured human keratinocytes in single cell suspension: A comparative in vitro study of different application techniques. Burns. 2008;34(2):212-219.
  10. Magnusson M, et al. Cultured autologous keratinocytes in suspension accelerate epithelial maturation in an in vivo wound model shown by surface electrical capacitance (SEC) and transepidermal water loss (TEWL). Plast Reconstr Surg. 2007;119(2):495-459.
  11. Green H. Regeneration of the skin after grafting of epidermal cultures. Lab Invest. 1989 May;60(5):583-584.
  12. Phillips TJ, Kehinde O, Green H, Gilchrest BA. Treatment of skin ulcers with cultured epidermal allografts. J Am Acad Dermatol. 1989 Aug;21(2 Pt 1):191-199.
  13. Auxenfans C, et al. Use of allogenic epidermal sheets for diffi cult wound healing: selection and testing of relevant growth factors. Biomed Mater Eng. 2006;16(4):73-83.
  14. Lootens L, et al. Keratinocytes in the treatment of severe burn injury: an update. Int Wound J. 2013;10(1):6-12.
  15. McHeik JN, et al. Quantitative and qualitative study in keratinocytes from foreskin in children: perspective application in paediatric burns. Burns. 2010;36(8):1277-1282.
  16. Palaiologou AA, Yukna RA, Moses R, Lallier ТЕ. Gingival, dermal, and periodontal ligament fi broblasts express different extracellular matrix receptors. J Periodontol. 2001;72(6):798-807.
  17. Sarkisov DS, Tumanov VP, Glushchenko EV et al. The use of cultured fibroblasts in the treatment of burned. Bulletin of Experimental Biology and Medicine. 1990;3:400-402. (In Russ.)
  18. Sarkisov DS, Alekseev AA, et al. Theoretical and practical aspects of the use of cultured fibroblasts in skin integrity regeneration. Herald RAMS. 1994;7:6-11. (In Russ.)
  19. Kolokoltsova TD, Yurchenko ND, Kolosov NG et al. Prospects of the use of human fetal fibroblasts in the treatment of wounds of various etiologies. Herald RAMS. 1998;3:32-35;28. (In Russ.)
  20. Zorin VL, Zorina AI, Petrakova OS, Cherkasov VR. Dermal fibroblasts for the treatment of skin defects. Genes and cells. 2009;4. (In Russ.) http://cyberleninka.ru/article/n/dermalnye-fibroblasty-dlya-lecheniya-defektov-kozhi
  21. Alekseev AA, et al. Combined autodermoplasty with transplantation of cultured fibroblasts with extensive deep burns: clinical results and perspectives. International symposium «New methods of treating burns using cultured skin cells». Tula. 1996;1-3. (In Russ.)
  22. Budkevich LI, et al. Results of cultured allofibroblasts application in children with deep burns. Abstracts of the session «Substitution Cell Therapy» of the V International symposium on aesthetic medicine. 2006. (In Russ.)
  23. Larionova KD, Kislitsyn PV, Kvitsinskaya NA. Application of cultured allofibroblasts in the treatment of dermal burns in children. Ques traumatol orthoped. 2011;1. (In Russ.)
  24. Billingham RE, Reynolds J. Transplantation studies on sheets of pure epidermal epithelium and on epidermal cell suspensions. Br J Plast Surg. 1952 Apr;5(1):25-36.
  25. Barker C. In memoriam: Rupert E. Billingham (1921—2002). Transplantation. 2003 Oct 15;76(7):1138-1143.
  26. Wood FM, et al. The use of a non-cultured autologous cell suspension and Integra dermal regeneration template to repair full-thickness skin wounds in a porcine model: a one-step process. Burns. 2007;33(6):693-700.
  27. Conti E, Wood F, Leclerc-Chalvet M. ReCell: Indications, Clinical Practice and Expected Outcomes. J Wound Tech. 2009;4:59-62.
  28. Vinnik YuS, Salmina AB, Drobushevskaya AI, et al. The cell technologies and the tissue engineering are for healing chronic wounds. Vestnik of experimental and clinical surgery. 2011;4:392-397. (In Russ.)
  29. Chakrabarty KH, Dawson RA, Harris P, et al. Development of autologous human dermal-epidermal composites based on sterilized human allodermis for clinical use. Br J of Dermatology. 1999;141:811-823.
  30. Gibbs S, van den Hoogenband HM, Kirtschig G, et al. Autologous full-thickness skin substitutes for healing chronic wounds. Br J of Dermatology. 2006;155:267-274.
  31. Vriens AP, Waaijman T, Gibbs S, et al. Comparison of autologous full-thickness gingiva and skin substitutes for wound healing. Cell Transplantation. 2008;17:1199-1209.
  32. Zorin VL, Zorina AI, Cherkasov VR. The analysis of the regeneration medicine foreign market. Cell transplantology and tissue engineering. 2009;3:68-78. (In Russ.)
  33. Gavrilenko AV, Pavlova ОV, Vachratyan PE. The use of fibroblasts and keratinocytes in complex treatment of venous trophic ulcers. Surgery. N.I. Pirogov Journal. 2008;10:80-83. (In Russ.)
  34. Bello YM, Falabella AF, Eaglstein WH. Tissue-engineered skin in wound healing. Am J Dermatol. 2000;2(5):305-315.
  35. Burke JF. Observation on the development of an artifi cial skin: pPresidential address 1982. J Trauma. 1983;6:543-551.
  36. Saap LJ, Donohue K, Falanfa V. Clinical classifi cation of bioengineered skin use and its correlation with healing of diabetic and venous ulcers. Dermatol Surg. 2004;30:8:1095-1100.
  37. Bell E, Sher S, Hull B, et al. The reconstitution of living skin. J Invest Dermatol. 1983 Jul;81(1 Suppl):2s-10s.
  38. Steffens D, Mathor MB, Santi BT, et al. Development of a biomaterial associated with mesenchymal stem cells and keratinocytes for use as a skin substitute. Regen Med. 2015 Nov;10(8):975-987.
  39. Wahl EA, Fierro FA, Peavy TR, et al. In Vitro evaluation of scaffolds for the delivery of mesenchymal stem cells to wounds. Biomed Res Int. 2015;2015:108571.
  40. Sánchez-Muñoz I, Granados R, Holguín Holgado P, et al. The use of adipose mesenchymal stem cells and human umbilical vascular endothelial cells on a fibrin matrix for endothelialized skin substitute. Tissue Eng Part A. 2015 Jan;21(1-2):214-223.
  41. Jiang LW, Chen H, Lu H. Using human epithelial amnion cells in human de-epidermized dermis for skin regeneration. J Dermatol Sci. 2016 Jan;81(1):26-34.
  42. Kisch T, Weber C, Rapoport DH, et al. LPS-stimulated human skin-derived stem cells enhance neo-vascularization during dermal regeneration. PLoS One. 2015 Nov 13;10(11):e0142907.
  43. Murphy SV, Atala A. 3D-bioprinting of tissues and organs. Nat Biotechnol. 2014 Aug;32(8):773-785.
  44. Suhikh GT. Mesenchymal stem cells. Bulletin of Experimental Biology and Medicine. 2002;133:2:124-131. (In Russ.)
  45. Matousková E, Broz L, Stolbová V, et al. Human allogeneic keratinocytes cultured on acellular xenodermis: the use in healing of burns and other skin defects. Biomed Mater Eng. 2006;16(4 Suppl):S63-S71.
  46. Ahn JI, Lee DH, Ryu YH, et al. Reconstruction of rabbit corneal epithelium on lyophilized amniotic membrane using the tilting dynamic culture method. Artif Organs. 2007 Sep;31(9):711-721.
  47. Lu SH, Sacks MS, Chung SY, et al. Biaxial mechanical properties of muscle-derived cell seeded small intestinal submucosa for bladder wall reconstitution. Biomaterials. 2005 Feb;26(4):443-449.
  48. Prichard HL, Reichert WM, Klitzman B. Adult adipose-derived stem cell attachment to biomaterials. Biomaterials. 2007 Feb;28(6):936-946.
  49. Altman AM, Matthias N, Yan Y, et al. Dermal matrix as a carrier for in vivo delivery of human adipose-derived stem cells. Biomaterials. 2008 Apr;29(10):1431-1442.
  50. Wang Y, Xu R, Luo G, et al. Biomimetic fibroblast-loaded artificial dermis with «sandwich» structure and designed gradient pore sizes promotes wound healing by favoring granulation tissue formation and wound re-epithelialization. Acta Biomater. 2016 Jan;30:246-257.
  51. Wahl EA, Fierro FA, Peavy TR, et al. In vitro evaluation of scaffolds for the delivery of mesenchymal stem cells to wounds. Biomed Res Int. 2015;2015:108571.
  52. Steffens D, Mathor MB, Santi BT, et al. Development of a biomaterial associated with mesenchymal stem cells and keratinocytes for use as a skin substitute. Regen Med. 2015 Nov;10(8):975-987.
  53. Romanova OA, Grigor’ev TE, Goncharov ME, et al. Chitosan as a modifying component of artificial scaffold for human skin tissue engineering. Bull Exp Biol Med. 2015 Aug;159(4):557-566.
  54. Mahjour SB, Fu X, Yang X, et al. Rapid creation of skin substitutes from human skin cells and biomimetic nanofibers for acute full-thickness wound repair. Burns. 2015 Dec;41(8):1764-1774.
  55. Lloyd C, Besse J, Boyce S. Controlled-rate freezing to regulate the structure of collagen-glycosaminoglycan scaffolds in engineered skin substitutes. J Biomed Mater Res B Appl Biomater. 2015 May;103(4):832-840.
  56. Shevchenko RV, et al. A review of tissue-engineered skin bioconctructs available for skin reconstruction. Journal of the royal Society Interface. 2010;7:229-258.
  57. Brohem СA, et al. Artificial skin in perspective: concepts and applications. Pigment Cell Melanoma Res. 2010;24:35-50.
  58. Eldad A, Stern Z, Sover H, et al. The cost of an extensive burn survival. Burns. 1993 Jun;19(3):235-238.
  59. Hansbrough JF. The promises of excisional therapy of burn wounds: have they been achieved? J Intensive Care Med. 1994 Jan;9(1):1-3.
  60. Zinovieva NA, Melerzanov AV, Petersen EV, et al. Application of GAL-KO transgenic pigs in: problems and prospects for the. Sel’skokhozyaistvennaya Biologiya [Agricultural Biology]. 2014;2:42-49.
  61. Leto Barone AA, Mastroianni M, Farkash EA, et al. Genetically modified porcine split-thickness skin grafts as an alternative to allograft for provision of temporary wound coverage: preliminary characterization. Burns. 2015 May;41(3):565-574.
  62. Vinnik YuS, Salmina AB, Drobushevskaya AI, et al. The cell technologies and the tissue engineering are for healing chronic wounds. Vestnik of experimental and clinical surgery 2011;4:2:392-397. (In Russ.)
  63. Still J, et al. The use of a collagen sponge/living cell composite material to treat donor sites in burn patients. Burns. 2003;29:837-841.
  64. Wisser D, Steffes J. Skin replacement with a collagen based dermal substitute, autologous keratinocytes and fi broblasts in burn trauma. Burns. 2003;29(4):375-380.
  65. Llames SG, et al. Human plasma as a dermal scaffold for the generation of a completely autologous bioengineered skin. Transplantation. 2004;77(3): 350-355.
  66. Khodadadi L, et al. Cell therapy in burn repair. Yakhteh Med J. 2008;10(3):167-178.
  67. Patel M, Fisher J. Biomaterial scaffolds in pediatric tissue engineering. Pediatr Res. 2008;63:497-501.
  68. Pham C, et al. Bioengineered skin substitutes for the management of burns: a systematic review. Burns. 2007;33:946-957.
  69. Auxenfans C, et al. Evolution of three dimensional skin equivalent models reconstructed in vitro by tissue engineering. Eur J Dermatol. 2009;19(2):107-113.
  70. Ehrlich HP. Understanding experimental biology of skin equivalent: from laboratory to clinical use in patients with burns and chronic wounds. Am J Surg. 2004;187(5A):29-33.
  71. Goodman G. An automated autologous cell transplantation method for treatment of hypopigmented scarring. Dermatology Surgery. 2008;34:579-581.
  72. Ehrlich HP. Understanding experimental biology of skin equivalent: from laboratory to clinical use in patients with burns and chronic wounds. Am J Surg. 2004;187(5A):29-33.
  73. Mazzoleni G, Di Lorenzo D, Steimberg N. Modelling tissues in 3D: the next future of pharmaco-toxicology and food research? Genes Nutr. 2009;4:13-22.
  74. Horning JL, Sahoo SK, Vijayaraghavalu S, et al. 3D-tumor model for in vitro evaluation of anticancer drugs. Mol Pharm. 2008;5:849-862.
  75. Grinnell F. Fibroblast mechanics in three-dimensional collagen matrices. J Bodyw Mov Ther. 2008;12:191-193.
  76. El Ghalbzouri A, Gibbs S, Lamme E, et al. Effect of fibroblasts on epidermal regeneration. Br J Dermatol. 2002a;147:230-243.
  77. Stark HJ, Szabowski A, Fusenig NE, Maas-Szabowski N. Organotypic cocultures as skin equivalents: a complex and sophisticated in vitro system. Biol Proced Online. 2004a;6:55-60.
  78. Wong T, McGrath JA, Navsaria H. The role of fibroblasts in tissue engineering and regeneration. Br J Dermatol. 2007;156:1149-1155.
  79. Kolokolchikova EG, Budkevich LI, Bobrovnikov AE, Tumanov VP. Morphological changes of burn wounds after allogeneic fibroblasts transplantation. Bulletin of experimental biology. 2001;1:107-111. (In Russ.)
  80. Chekmareva IA, Vtyurin BV, Adamyan AA, et al. Specific influence of epidermal growth factor immobilized in the wound coating with soluble collagen on wound healing in the experiment. Bulletin of Experimental Biology and Medicine. 2000;129:465-469. (In Russ.)
  81. Berk GS, Blyumin DKh, Sebastian DL. Regeneration of vocal cords with cultured fibroblasts. Bulletin of Experimental Biology and Medicine. 2000;130(8):207-209. (In Russ.)
  82. Keller G, Sebastian D, Revazova E. Preservation of injected autologous human fibroblasts. Bulletin of Experimental Biology and Medicine. 2000;130(8):203-206. (In Russ.)
  83. Konstantinova NV, Duong DM, Remenyik E, et al. Interleukin-8 is induced in skin equivalents and is highest in those derived from psoriatic fibroblasts. J Invest Dermatol. 1996;107:615-621.
  84. Barker CL, McHale MT, Gillies AK, et al. The development and characterization of an in vitro model of psoriasis. J Invest Dermаtol. 2004;123:892-901.
  85. Gazel A, Rosdy M, Bertino B, et al. A characteristic subset of psoriasis-associated genes is induced by oncostatin-M in reconstituted epidermis. J Invest Dermatol. 2006;126:2647-2657.
  86. Tjabringa G, Bergers M, van Rens D, et al. Development and validation of human psoriatic skin equivalents. Am J Pathol. 2008;173:815-823.
  87. Bessou S, Gauthier Y, Surlève-Bazeille JE, et al. Epidermal reconstructs in vitiligo: an extrinsic factor is needed to trigger the disease. Br J Dermatol. 1997;137:890-897.
  88. Cario-André M, Pain C, Gauthier Y, et al. In vivo and in vitro evidence of dermal fibroblasts influence on human epidermal pigmentation. Pigment Cell Res. 2006;19:434-442.
  89. Cario-André M, Pain C, Gauthier Y, Taïeb A. The melanocytorrhagic hypothesis of vitiligo tested on pigmented, stressed, reconstructed epidermis. Pigment Cell Res. 2007;20:385-393.
  90. Chiu LL, Sun CH, Yeh AT, et al. Photodynamic therapy on keloid fibroblasts in tissue-engineered keratinocyte-fibroblast co-culture. Lasers Surg Med. 2005;37:231-244.
  91. Butler PD, Ly DP, Longaker MT, Yang GP. Use of organotypic coculture to study keloid biology. Am J Surg. 2008;195:144-148.
  92. Gontier E, Cario-André M, Lepreux S, et al. Dermal nevus cells from congenital nevi cannot penetrate the dermis in skin reconstructs. Pigment Cell Res. 2002;15:41-48.
  93. Gontier E, Cario-André M, Vergnes P, et al. The role of E-cadherin in nevogenesis: an experimental study using epidermal reconstructs. Exp Dermatol. 2004;13:326-331.
  94. Bernerd F, Asselineau D. An organotypic model of skin to study photodamage and photoprotection in vitro. J Am Acad Dermatol. 2008;58:S155-S159.
  95. Ergün SS, Cek DI, Demirkesen C. Is facial resurfacing with monobloc full-thickness skin graft a remedy in xeroderma pigmentosum? Plast Reconstr Surg. 2002;110:1290-1293.
  96. Bergoglio V, Warrick E, Chevallier-Lagente O, Magnaldo T. Cutaneous gene therapy: the graft takes. Med Sci (Paris). 2008;24:607-614.
  97. Herlin C, Saunière D, Huertas D. Xeroderma pigmentosum: radical therapeutic procedure on the face using artificial dermal. Ann Chir Plast Esthet. 2009;54:594-599.
  98. Ferrari S, Pellegrini G, Matsui T, et al. Gene therapy in combination with tissue engineering to treat epidermolysis bullosa. Expert Opin Biol Ther. 2006;6:367-378.
  99. Mavilio F, Pellegrini G, Ferrari S, et al. Correction of junctional epidermolysis bullosa by transplantation of genetically modified epidermal stem cells. Nat Med. 2006;12:1397-1402.
  100. De Luca M, Pellegrini G, Mavilio F. Gene therapy of inherited skin adhesion disorders: a critical overview. Br J Dermatol. 2009;161:19-24.
  101. Brohem СA, et al. Artificial skin in perspective: concepts and applications. Pigment Cell Melanoma Res. 2010;24:35-50.
  102. Welss T, Basketter DA, Schröder KR. In vitro skin irritation: facts and future. State of the art review of mechanisms and models. Toxicol In Vitro. 2004;18:231-243.
  103. Curren RD, Mun GC, Gibson DP, Aardema MJ. Development of a method for assessing micronucleus induction in a 3D-human skin model (EpiDerm). Mutat Res. 2006;607:192-204.
  104. Hu T, Kaluzhny Y, Mun GC, et al. Intralaboratory and interlaboratory evaluation of the EpiDerm 3D-human reconstructed skin micronucleus (RSMN) assay. Mutat Res. 2009;673:100-108.

Close metadata

Contact the author
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.

Email Confirmation

An email was sent to test@gmail.com with a confirmation link. Follow the link from the letter to complete the registration on the site.

Email Confirmation

Contact us
If you have any questions, complaints or suggestions, please use this feedback form.
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.
Submit Application

You can become an author of one of our magazines, send a request and we will consider it in during the day.

Name
Phone
E-mail
Message
Login
Registration
E-mail
Password
Forgot Password?

Log in to the site using your account in one of the services

Password recovery
E-mail
Login
Register

We use cооkies to improve the performance of the site. By staying on our site, you agree to the terms of use of cооkies. To view our Privacy and Cookie Policy, please. click here.