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Nadeev A.P.

Novosibirsk State Medical University

Kliver V.E.

Novosibirsk State Medical University;
National Medical Research Center named after Academician E. N. Meshalkin

Volkov A.M.

National Medical Research Center named after Academician E. N. Meshalkin

Fomichev A.V.

National Medical Research Center named after Academician E. N. Meshalkin

Sirota D.A.

Novosibirsk State Medical University;
National Medical Research Center named after Academician E. N. Meshalkin

Zhulkov M.O.

National Medical Research Center named after Academician E. N. Meshalkin

Kliver E.E.

Novosibirsk State Medical University;
National Medical Research Center named after Academician E. N. Meshalkin

Volchek A.V.

Novosibirsk State Medical University

Kazanskaya G.M.

Federal Research Center for Fundamental and Translational Medicine

Aidagulova S.V.

Novosibirsk State Medical University;
Federal Research Center for Fundamental and Translational Medicine

Histological and ultrastructural analysis of biopsy specimens of donor heart under conditions of extended period of pharmaco-cold ischaemia

Authors:

Nadeev A.P., Kliver V.E., Volkov A.M., Fomichev A.V., Sirota D.A., Zhulkov M.O., Kliver E.E., Volchek A.V., Kazanskaya G.M., Aidagulova S.V.

More about the authors

Journal: Russian Journal of Archive of Pathology. 2024;86(5): 33‑41

DOI: 10.17116/patol20248605133

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To cite this article:

Nadeev AP, Kliver VE, Volkov AM, et al. . Histological and ultrastructural analysis of biopsy specimens of donor heart under conditions of extended period of pharmaco-cold ischaemia. Russian Journal of Archive of Pathology. 2024;86(5):33‑41. (In Russ.)
https://doi.org/10.17116/patol20248605133

Подписка 2025 (Russian Journal of Archive of Pathology)
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OBJECTIVE

At the histological and ultramicroscopic level, compare biopsy specimens of donor heart under standard (up to 240 min) and extended (more than 240 min) periods of pharmaco-cold preservation.

MATERIAL AND METHODS

Biopsy specimens of the left atrium of donor hearts: group 1 — 8 samples after transportation with pharmaco-cold preservation of the graft in Bretschneider solution (Dr. Franz Köhler Chemie GmbH, Germany) up to 240 min, (Me 140), and group 2 — 5 samples after an extended pharmaco-cold period (more than 240 min; Me 375) were examined using light microscopy of semi-thin sections and transmission electron microscopy, followed by stereological and statistical analysis.

RESULTS

A comparative study of the myocardium of donor hearts revealed stereotypical dystrophic changes in cardiomyocytes. Semi-thin sections demonstrated a mosaic pattern of myocardial parenchyma in both groups, caused by contracture and less pronounced lytic changes in myocytes, which were accompanied by stromal edema without statistically significant differences according to stereological studies. Ultrathin sections of the perinuclear zones of cardiomyocytes visualized reduction and focal damage to myofibrils and mitochondria in combination with pronounced autophagy; at the same time, with a shorter duration of the pharmaco-cold period, the stereological indicators of cardiomyocyte organelles indicated a relatively better supply of myofibrils with mitochondria.

CONCLUSION

The results obtained suggest a sufficiently high degree of preservation of the tissue and ultrastructural organization of donor hearts with prolonged (more than 240 min) pharmaco-cold ischemia to restore adequate cardiac activity after heart transplantation.

Keywords:

donor heart
atrial biopsy
pharmaco-cold preservation
cardiomyocytes
transmission electron microscopy
stereology

Authors:

Nadeev A.P.

Novosibirsk State Medical University

Kliver V.E.

Novosibirsk State Medical University;
National Medical Research Center named after Academician E. N. Meshalkin

Volkov A.M.

National Medical Research Center named after Academician E. N. Meshalkin

Fomichev A.V.

National Medical Research Center named after Academician E. N. Meshalkin

Sirota D.A.

Novosibirsk State Medical University;
National Medical Research Center named after Academician E. N. Meshalkin

Zhulkov M.O.

National Medical Research Center named after Academician E. N. Meshalkin

Kliver E.E.

Novosibirsk State Medical University;
National Medical Research Center named after Academician E. N. Meshalkin

Volchek A.V.

Novosibirsk State Medical University

Kazanskaya G.M.

Federal Research Center for Fundamental and Translational Medicine

Aidagulova S.V.

Novosibirsk State Medical University;
Federal Research Center for Fundamental and Translational Medicine

Received:

23.04.2024

Accepted:

26.06.2024

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