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Romanishkin I.D.

Prokhorov Institute of General Physics

Ospanov A.

National Research Nuclear University

Savelyeva T.A.

Prokhorov Institute of General Physics;
National Research Nuclear University

Shugai S.V.

Burdenko Neurosurgical Center

Goryainov S.A.

Burdenko Neurosurgical Center

Pavlova G.V.

Burdenko National Medical Research Center of Neurosurgery

Pronin I.N.

Burdenko Neurosurgical Center

Loshchenov V.B.

Prokhorov Institute of General Physics;
National Research Nuclear University

Multimodal method of tissue differentiation in neurooncology using Raman spectroscopy, fluorescence and diffuse reflectance spectroscopy

Authors:

Romanishkin I.D., Ospanov A., Savelyeva T.A., Shugai S.V., Goryainov S.A., Pavlova G.V., Pronin I.N., Loshchenov V.B.

More about the authors

Journal: Burdenko's Journal of Neurosurgery. 2022;86(5): 5‑12

DOI: 10.17116/neiro2022860515

Read: 2170 times

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Table of contents

MULTIMODAL METHOD OF TISSUE DIFFERENTIATION IN NEUROONCOLOGY USING RAMAN SPECTROSCOPY, FLUORESCENCE AND DIFFUSE REFLECTANCE SPECTROSCOPY
MULTIMODAL METHOD OF TISSUE DIFFERENTIATION IN NEUROONCOLOGY USING RAMAN SPECTROSCOPY, FLUORESCENCE AND DIFFUSE REFLECTANCE SPECTROSCOPY

To cite this article:

Romanishkin ID, Ospanov A, Savelyeva TA, et al. . Multimodal method of tissue differentiation in neurooncology using Raman spectroscopy, fluorescence and diffuse reflectance spectroscopy. Burdenko's Journal of Neurosurgery. 2022;86(5):5‑12. (In Russ., In Engl.)
https://doi.org/10.17116/neiro2022860515

Подписка 2025 Журнал «Вопросы нейрохирургии» имени Н.Н. Бурденко (Burdenko's Journal of Neurosurgery)
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

BACKGROUND

There is a need to expand the possibilities of urgent analysis of intracranial tumor type during resection. These measures are necessary to improve resection quality with preservation of intact tissues and avoiding recurrence and neurological impairment in postoperative period.

OBJECTIVE

To create optical-spectral method for differentiating the intracranial tumor types.

MATERIAL AND METHODS

We used a combination of certain methods such as fluorescence spectroscopy to analyze the content of endogenous and exogenous fluorophores in samples, diffuse reflectance spectroscopy to analyze structural integrity of tissues according to light scattering and blood filling according to hemoglobin spectrum absorption, as well as spontaneous Raman spectroscopy detecting individual molecular components of tissues. The study was conducted at the Laboratory of Neurosurgical Anatomy and Conservation of Biological Materials of the Burdenko Neurosurgical Center and included 93 tissue samples from 60 patients diagnosed with glioblastoma (n=28), meningioma (n=12), astrocytoma (n=9), oligodendroglioma (n=5), and metastasis (n=6).

RESULTS

Different types of intracranial tumors that cannot be differentiated using one of the considered spectroscopy modes can be distinguished in another one. Thus, we can conclude possible advantages of combined optical-spectral approach.

Keywords:

spectroscopy
fluorescence
diffuse reflectance
Raman scattering
intracranial tumors

Authors:

Romanishkin I.D.

Prokhorov Institute of General Physics

Ospanov A.

National Research Nuclear University

Savelyeva T.A.

Prokhorov Institute of General Physics;
National Research Nuclear University

Shugai S.V.

Burdenko Neurosurgical Center

Goryainov S.A.

Burdenko Neurosurgical Center

Pavlova G.V.

Burdenko National Medical Research Center of Neurosurgery

Pronin I.N.

Burdenko Neurosurgical Center

Loshchenov V.B.

Prokhorov Institute of General Physics;
National Research Nuclear University

Received:

28.07.2022

Accepted:

19.08.2022

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