Microglia-neuron interactions in the caudate nucleus in different course of schizophrenia

Vikhreva O.V.; Rakhmanova V.I.; Uranova N.A.

doi:https://doi.org/10.17116/jnevro2024124071154

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Abstract:

OBJECTIVE

To study the ultrastructure of microglia and neurons in contact with each other in the head of the caudate nucleus in continuous schizophrenia (CS) and paroxysmal-progressive schizophrenia (PPS) as compared to controls and to analyze correlations between the parameters of microglia and neurons in the control and schizophrenia groups.

MATERIAL AND METHODS

Post-mortem electron microscopic morphometric study of microglia and neurons in contact with each other was performed in the head of the caudate nucleus in 9 cases of CS, 10 cases of PPS and 20 controls without mental pathology. Group comparisons were made using analysis of covariance and Pearson correlation analysis.

RESULTS

The PPS group showed increased numerical density of microglia in young (≤50 years old) patients compared to elderly (>50 years old) controls and increased area of endoplasmic reticulum vacuoles in microglia in young patients compared to young controls. Decreased numerical density of microglia was found in the CS group compared to the PPS group (p<0.05), and increased volume fraction (Vv) and the number of lipofuscin granules in microglia were found in the CS group in elderly patients compared with young and elderly controls. In this group, negative correlations were revealed between the numerical density of microglia, microglia nuclear area and the duration of disease (r= –0.72, p=0.03; r= –0.8; p=0.01). Decreased Vv and the number of mitochondria in microglia and increased area and perimeter of neurons were revealed in both groups compared to the control group. In neurons, increased vacuole area was found in the PPS group and mitochondrial area in the NTS group compared to the control group. Correlation violations were found between the parameters of mitochondria in microglia and neurons in both PPS and CS groups and between the area of mitochondria in neurons and the area of vacuoles in microglia in the CS group compared to the control group.

CONCLUSION

Disturbed interactions between microglia and neurons in the caudate nucleus are associated with the types of course of schizophrenia and with microglial reactivity. They might be caused by the damage of energy metabolism in microglia in both types of schizophrenia course and by stress of endoplasmic reticulum in microglia in CS.

Keywords:

microglia

neuron

caudate nucleus

continuous schizophrenia

paroxysmal-progressive schizophrenia

Authors:

Vikhreva O.V.

Mental Health Research Center

ORCID: 0000-0002-6920-316X

Rakhmanova V.I.

Mental Health Research Center

ORCID: 0000-0002-9484-8154

Uranova N.A.

Mental Health Research Center

ORCID: 0000-0001-9543-7421

Received:

27.02.2024

Accepted:

02.04.2024

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References:

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