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Safonova T.N.

Krasnov Research Institute of Eye Diseases

Novikov I.A.

Krasnov Research Institute of Eye Diseases;
Prokhorov General Physics Institute of the Russian Academy of Sciences

Zaitseva G.V.

Krasnov Research Institute of Eye Diseases

Dobretsova E.A.

Prokhorov General Physics Institute of the Russian Academy of Sciences

Solodovnikov V.I.

Center of Information Technologies in Design of the Russian Academy of Sciences

Possibilities of optical fluorescence spectral decomposition of meibum

Authors:

Safonova T.N., Novikov I.A., Zaitseva G.V., Dobretsova E.A., Solodovnikov V.I.

More about the authors

Journal: Russian Annals of Ophthalmology. 2025;141(5): 29‑36

DOI: 10.17116/oftalma202514105129

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Table of contents

POSSIBILITIES OF OPTICAL FLUORESCENCE SPECTRAL DECOMPOSITION OF MEIBUM
POSSIBILITIES OF OPTICAL FLUORESCENCE SPECTRAL DECOMPOSITION OF MEIBUM

To cite this article:

Safonova TN, Novikov IA, Zaitseva GV, Dobretsova EA, Solodovnikov VI. Possibilities of optical fluorescence spectral decomposition of meibum. Russian Annals of Ophthalmology. 2025;141(5):29‑36. (In Russ.)
https://doi.org/10.17116/oftalma202514105129

Подписка 2026 Вестник офтальмологии (Russian Annals of Ophthalmology)
МСФ на ЯМ
Использование инфографики авторами научных работ
Abstract:

OBJECTIVE

The is study evaluated the possibilities of using optical fluorescence to determine the components of meibum.

MATERIAL AND METHODS

Meibum samples from 16 volunteers were analyzed using fluorescence spectroscopy in the visible optical range. The meibum spectrum was decomposed into three spectral components corresponding to the fluorescence of three reference substances: native keratin, lactoferrin, and riboflavin-mononucleotide. Each meibum sample was characterized by the spectral fraction of each of the three dominant fluorophores.

RESULTS

The meibum spectra of 16 studied samples were obtained by fluorescence spectroscopy in the visible optical range under 440 nm laser excitation. Fluorescence of the meibum sample was detected in all cases. Using the proposed algorithm, the relative spectral component of one of the proposed reference substances was calculated for each real spectrum.

CONCLUSION

Fluorescence spectroscopy in the visible optical range, alongside other methods, allows the identification of major compartments constituting the bulk of meibum mass.

Keywords:

meibomian glands
meibum
fluorescence spectroscopy
proteomic analysis of meibum
keratin
lactoferrin
flavin

Authors:

Safonova T.N.

Krasnov Research Institute of Eye Diseases

Novikov I.A.

Krasnov Research Institute of Eye Diseases;
Prokhorov General Physics Institute of the Russian Academy of Sciences

Zaitseva G.V.

Krasnov Research Institute of Eye Diseases

Dobretsova E.A.

Prokhorov General Physics Institute of the Russian Academy of Sciences

Solodovnikov V.I.

Center of Information Technologies in Design of the Russian Academy of Sciences

Received:

29.01.2025

Accepted:

18.06.2025

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