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Khalatyan A.S.

Research Institute of Eye Diseases

Kholina E.G.

Lomonosov Moscow State University

Strakhovskaya M.G.

Lomonosov Moscow State University;
Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies of the Federal Medical and Biological Agency

Budzinskaya M.V.

Krasnov Research Institute of Eye Diseases

Shevlyagina N.V.

Gamaleya National Research Center for Epidemiology and Microbiology

Zhukhovitsky V.G.

N.F. Gamaleya Federal research Center for Epidemiology and Microbiology of the Ministry of Public Health of Russia;
Sechenov the First Moscow State Medical University of the Ministry of Public Health

Antibacterial effect of the antiseptic picloxydine dihydrochloride on conjunctival isolates of gram-negative bacteria

Authors:

Khalatyan A.S., Kholina E.G., Strakhovskaya M.G., Budzinskaya M.V., Shevlyagina N.V., Zhukhovitsky V.G.

More about the authors

Journal: Russian Annals of Ophthalmology. 2021;137(5‑2): 238‑247

DOI: 10.17116/oftalma2021137052238

Read: 4162 times

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Table of contents

ANTIBACTERIAL EFFECT OF THE ANTISEPTIC PICLOXYDINE DIHYDROCHLORIDE ON CONJUNCTIVAL ISOLATES OF GRAM-NEGATIVE BACTERIA
ANTIBACTERIAL EFFECT OF THE ANTISEPTIC PICLOXYDINE DIHYDROCHLORIDE ON CONJUNCTIVAL ISOLATES OF GRAM-NEGATIVE BACTERIA

To cite this article:

Khalatyan AS, Kholina EG, Strakhovskaya MG, Budzinskaya MV, Shevlyagina NV, Zhukhovitsky VG. Antibacterial effect of the antiseptic picloxydine dihydrochloride on conjunctival isolates of gram-negative bacteria. Russian Annals of Ophthalmology. 2021;137(5‑2):238‑247. (In Russ.)
https://doi.org/10.17116/oftalma2021137052238

Подписка 2026 Вестник офтальмологии (Russian Annals of Ophthalmology)
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

The preoperative and postoperative use of antiseptics can be an alternative to antibiotics in repeated courses of anti-VEGF therapy for reducing the risk of developing antibiotic resistance in eye microflora. Among gram-negative bacteria, the most frequently isolated pathogen that causes eye infections is Pseudomonas aeruginosa, which is characterized by reduced sensitivity to antibiotics and disinfectants.

PURPOSE

To study the effect of the antiseptic picloxydine dihydrochloride on the gram-negative bacteria Escherichia coli, Pseudomonas luteola and P. aeruginosa isolated from the conjunctiva.

MATERIAL AND METHODS

The identification of bacterial isolates and study of their sensitivity to antibiotics were carried out using the automated bacteriological analyzer BD Phoenix 100. To determine the bactericidal concentration, the method of serial dilutions of the antiseptic in a liquid nutrient medium was used. The binding of cationic molecules of picloxydine dihydrochloride to bacterial cells was detected by neutralizing the bacterial surface with increasing amounts of antiseptic, and measuring the zeta potential on the Zetasizer Nano ZS analyzer. The ultrastructure of bacterial cells was studied using the two-beam scanning ion-electron microscope Quanta 200 3D.

RESULTS

The most resistant was P. aeruginosa. The interaction mechanism of picloxydine dihydrochloride with bacterial cells includes electrostatic binding of positively charged antiseptic molecules to negatively charged cell walls. Picloxydine dihydrochloride has a destructive effect on the bacterial cell wall and plasma membrane, which leads to cell lysis and release of intracellular components.

CONCLUSION

Picloxydine dihydrochloride exhibits bactericidal activity against gram-negative conjunctival isolates and is promising for preventive use during repeated courses of intravitreal injections.

Keywords:

eye microflora
gram-negative bacteria
Pseudomonas aeruginosa
antiseptic picloxydine dihydrochloride

Authors:

Khalatyan A.S.

Research Institute of Eye Diseases

Kholina E.G.

Lomonosov Moscow State University

Strakhovskaya M.G.

Lomonosov Moscow State University;
Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies of the Federal Medical and Biological Agency

Budzinskaya M.V.

Krasnov Research Institute of Eye Diseases

Shevlyagina N.V.

Gamaleya National Research Center for Epidemiology and Microbiology

Zhukhovitsky V.G.

N.F. Gamaleya Federal research Center for Epidemiology and Microbiology of the Ministry of Public Health of Russia;
Sechenov the First Moscow State Medical University of the Ministry of Public Health

Received:

30.03.2021

Accepted:

20.05.2021

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References:

  1. Budzinskaya MV, Strakhovskaya MG, Andreeva IV, Khalatyan AS. Conjunctival microflora and its antibiotic sensitivity after serial intravitreal injections. Vestnik oftal’mologii. 2019;135(5-2):135-141. (In Russ.). https://doi.org/10.17116/oftalma2019135052135
  2. Khalatyan AS, Budzinskaya MV, Kholina EG, Strakhovskaya MG, Kolyshkina NA, Kovalenko IB, Zhukhovitsky VG. Sensitivity of antibiotic resistant coagulase-negative staphylococci to antiseptic picloxydin. Klinicheskaya praktika. 2020;11(1):42-48. (In Russ.). https://doi.org/10.17816/clinpract17543
  3. Budzinskaya MV, Khalatyan AS, Strakhovskaya MG, Zhukhovitsky VG. Ocular flora in patients undergoing intravitreal injections: antibiotic resistance patterns and susceptibility to antiseptic picloxydine. International Journal of Ophthalmology. 2020;13(1):85-92.  https://doi.org/10.18240/ijo.2020.01.13
  4. Baron EJ, Miller JM, Weinstein MP, Richter SS, Gilligan PH, Thomson RB Jr, Bourbeau P, Carroll KC, Kehl SC, Dunne WM, Robinson-Dunn B, Schwartzman JD, Chapin KC, Snyder JW, Forbes BA, Patel R, Rosenblatt JE, Pritt BS. A guide to utilization of the microbiology Laboratory for Diagnosis of infectious diseases: 2013 recommendations by the Infectious Diseases Society of America (IDSA) and the American Society for Microbiology (ASM). Clinical Infectious Diseases. 2013;57(4):22-121.  https://doi.org/10.1093/cid/cit278
  5. Harbarth S, Samore MH. Antimicrobial resistance determinants and future control. Emerging Infectious Diseases. 2005;11(6):798-801.  https://doi.org/10.3201/eid1106.050167
  6. Shimizu Y, Toshida H, Honda R, Ohta T, Asada Y, Murakami A. Prevalence of drug resistance and culture-positive rate among microorganisms isolated from patients with ocular infections over a 4-year period. Clinical Ophthalmology. 2013;7:695-702.  https://doi.org/10.2147/OPTH.S43323
  7. Jayahar Bharathi M, Ramakrishnan R, Ramesh S, Murugan N. Extended-spectrum beta-lactamase-mediated resistance among bacterial isolates recovered from ocular infections. Ophthalmic Research. 2012;47(1):52-56.  https://doi.org/10.1159/000322807
  8. Summaiya M, Neeta K, Sangita R. Ocular infections: rational approach to antibiotic therapy. National Journal of Medical Research. 2012;2(1):22-24. 
  9. Subedi D, Vijay AK, Willcox M. Overview of mechanisms of antibiotic resistance in Pseudomonas aeruginosa: an ocular perspective. Clinical and Experimental Optometry. 2018;101(2):162-171.  https://doi.org/10.1111/cxo.12621
  10. Willcox MD. Review of resistance of ocular isolates of Pseudomonas aeruginosa and staphylococci from keratitis to ciprofloxacin, gentamicin and cephalosporins. Clinical and Experimental Optometry. 2011;94(2):161-168.  https://doi.org/10.1111/j.1444-0938.2010.00536.x
  11. Abidi SH, Sherwani SK, Siddiqui TR, Bashir A, Kazmi SU. Drug resistance profile and biofilm forming potential of Pseudomonas aeruginosa isolated from contact lenses in Karachi-Pakistan. BMC Ophthalmology. 2013; 13:57.  https://doi.org/10.1186/1471-2415-13-57
  12. Mohammadinia M, Rahmani S, Eslami G, Ghassemi-Broumand M, Aghazadh Amiri M, Aghaie G, Tabatabaee SM, Taheri S, Behgozin A. Contact lens disinfecting solutions antibacterial efficacy: comparison between clinical isolates and the standard ISO ATCC strains of Pseudomonas aeruginosa and Staphylococcus aureus. Eye. 2012;26(2):327-330.  https://doi.org/10.1038/eye.2011.284
  13. Livermore DM. Multiple mechanisms of antimicrobial resistance in Pseudomonas aeruginosa: our worst nightmare? Clinical Infectious Diseases. 2002; 34(5):634-640.  https://doi.org/10.1086/338782
  14. Kung VL, Ozer EA, Hauser AR. The accessory genome of Pseudomonas aeruginosa. Microbiology and Molecular Biology Reviews: MMBR. 2010;74(4): 621-641.  https://doi.org/10.1128/mmbr.00027-10
  15. Conway T, Cohen PS. Commensal and pathogenic Escherichia coli metabolism in the gut. Microbiology Spectrum. 2015;3(3):10.  https://doi.org/10.1128/microbiolspec.mbp-0006-2014
  16. Sherwal BL, Verma AK. Epidemiology of ocular infection due to bacteria and fungus — a prospective study. JK Science: Journal of Medical Education and Research. 2008;10(3):127-131. 
  17. Gopinathan U, Sharma S, Garg P, Rao GN. Review of epidemiological features, microbiological diagnosis and treatment outcome of microbial keratitis: experience of over a decade. Indian Journal of Ophthalmology. 2009; 57(4):273-279.  https://doi.org/10.4103/0301-4738.53051
  18. Callegan MC, Engelbert M, Parke DW, Jet BD, Gilmore MS. Bacterial endophthalmitis: epidemiology, therapeutics, and bacterium-host interactions. Clinical Microbiology Reviews. 2002;15(1):111-124.  https://doi.org/10.1128/cmr.15.1.111-124.2002
  19. Jindal A, Pathengay A, Mithal K, Jalali S, Mathai A, Pappuru RR, Narayanan R, Chhablani J, Motukupally SR, Sharma S, Das T, Flynn HW. Endophthalmitis after open globe injuries: changes in microbiological spectrum and isolate susceptibility patterns over 14 years. Journal of Ophthalmic Inflammation and Infection. 2014;4(1):5.  https://doi.org/10.1186/1869-5760-4-5
  20. Murthy TA, Rangappa P, Rao S, Rao K. ESBL E. coli urosepsis resulting in endogenous panophthalmitis requiring evisceration of the eye in a diabetic patient. Case Reports in Infectious Diseases. 2015;2015:4.  https://doi.org/10.1155/2015/897245
  21. Stratton M, Capitena C, Christensen L, Paciuc-Beja M. Escherichia coli eyelid abscess in a patient with alcoholic cirrhosis. Case Reports in Ophthalmological Medicine. 2015;2015:2.  https://doi.org/10.1155/2015/827609
  22. Sharma S. Diagnosis of infectious diseases of the eye. Eye. 2012;26(2):177-184.  https://doi.org/10.1038/eye.2011.275
  23. Mishra SK, Basukala P, Basukala O, Parajuli K, Pokhrel BM, Rijal BP. Detection of biofilm production and antibiotic resistance pattern in clinical isolates from indwelling medical devices. Current Microbiology. 2015;70(1):128-134.  https://doi.org/10.1007/s00284-014-0694-5
  24. Jassim SH, Sivaraman KR, Jimenez JC, Jaboori AH, Federle MJ, de la Cruz J, Cortina MS. Bacteria colonizing the ocular surface in eyes with boston type 1 keratoprosthesis: analysis of biofilm-forming capability and vancomycin tolerance. Investigative Ophthalmology and Visual Science. 2015;56(8):4689-4696. https://doi.org/10.1167/iovs.15-17101
  25. Hoyle BD, Wong CK, Costerton JW. Disparate efficacy of tobramycin on Ca(2+)-, Mg(2+), and HEPES-treated Pseudomonas aeruginosa biofilms. Canadian Journal of Microbiology. 1992;38(11):1214-1218. https://doi.org/10.1139/m92-201
  26. Elder MJ, Stapleton F, Evans E, Dart JK. Biofilm-related infections in ophthalmology. Eye. 1995;9(1):102-109.  https://doi.org/10.1038/eye.1995.16
  27. Katiyar R, Vishwakarma A, Kaistha SD. Analysis of biofilm formation and antibiotic resistance of microbial isolates from intraocular lens following conventional extracapsular cataract surgery. International Journal of Research of Pure and Applied Microbiology. 2012;2(2):20-24. 
  28. Tanaka K, Shimada H, Mori R, Nakashizuka H, Hattori T, Okubo Y. No increase in incidence of post-intravitreal injection endophthalmitis without topical antibiotics: a prospective study. Japanese Journal of Ophthalmology. 2019;63(5):396-401.  https://doi.org/10.1007/s10384-019-00684-5
  29. Costello P, Bakri SJ, Beer PM, Singh RJ, Falk NS, Peters GB, Melendez JA. Vitreous penetration of topical moxifloxacin and gatifloxacin in humans. Retina. 2006;26(2):191-195.  https://doi.org/10.1097/00006982-200602000-00012
  30. Menchini F, Toneatto G, Miele A, Donati S, Lanzetta P, Virgili G. Antibiotic prophylaxis for preventing endophthalmitis after intravitreal injection: a systematic review. Eye. 2018;32(9):1423-1431. https://doi.org/10.1038/s41433-018-0138-8
  31. Li AL, Wykoff CC, Wang R, Chen E, Benz MS, Fish RH, Wong TP, Major JC Jr, Brown DM, Schefler AC, Kim RY, OʼMalley RE. Endophthalmitis after intravitreal injection: role of prophylactic topical ophthalmic antibiotics. Retina. 2016;36(7):1349-1356. https://doi.org/10.1097/iae.0000000000000901
  32. Storey P, Dollin M, Pitcher J, Reddy S, Vojtko J, Vander J, Hsu J, Garg SJ; Post-Injection Endophthalmitis Study Team. The role of topical antibiotic prophylaxis to prevent endophthalmitis after intravitreal injection. Ophthalmology. 2014;121(1):283-289.  https://doi.org/10.1016/j.ophtha.2013.08.037
  33. Kim SJ, Toma HS. Antimicrobial resistance and ophthalmic antibiotics: 1-year results of a longitudinal controlled study of patients undergoing intravitreal injections. Archives of Ophthalmology. 2011;129(9):1180-1188. https://doi.org/10.1016/j.ophtha.2010.12.014
  34. Moss JM, Sanislo SR, Ta CN. A prospective randomized evaluation of topical gatifloxacin on conjunctival flora in patients undergoing intravitreal injections. Ophthalmology. 2009;116(8):1498-1501. https://doi.org/10.1016/j.ophtha.2009.02.024
  35. Milder E, Vander J, Shah C, Garg S. Changes in antibiotic resistance patterns of conjunctival flora due to repeated use of topical antibiotics after intravitreal injection. Ophthalmology. 2012;119(7):1420-1424. https://doi.org/10.1016/j.ophtha.2012.01.016
  36. Cheung CS, Wong AW, Lui A, Kertes PJ, Devenyi RG, Lam WC. Incidence of endophthalmitis and use of antibiotic prophylaxis after intravitreal injections. Ophthalmology. 2012;119(8):1609-1614. https://doi.org/10.1016/j.ophtha.2012.02.014
  37. Shimada H, Hattori T, Mori R, Nakashizuka H, Fujita K, Yuzawa M. Minimizing the endophthalmitis rate following intravitreal injections using 0.25% povidone-iodine irrigation and surgical mask. Graefe’s Archive for Clinical and Experimental Ophthalmology. 2013;251(8):1885-1890. https://doi.org/10.1007/s00417-013-2274-y
  38. Merani R, McPherson ZE, Luckie AP, Gilhotra JS, Runciman J, Durkin S, Muecke J, Donaldson M, Aralar A, Rao A, Davies PE. Aqueous chlorhexidine for intravitreal injection antispepsis: a case series and review of the literature. Ophthalmology. 2016;123(12):2588-2594. https://doi.org/10.1016/j.ophtha.2017.01.032
  39. Gili NJ, Noren T, Törnquist E, Crafoord S, Bäckman A. Preoperative preparation of eye with chlorhexidine solution significantly reduces bacterial load prior to 23-gauge vitrectomy in Swedish health care. BMC Ophthalmology. 2018;18(1):167.  https://doi.org/10.1186/s12886-018-0844-9
  40. Ozkan J, Willcox MD. The Ocular Microbiome: Molecular Characterization of a Unique and Low Microbial Environment. Current Eye Research. 2019;44(7):685-694.  https://doi.org/10.1080/02713683.2019.1570526
  41. Hyon JY, Eser I, O’Brien TP. Kill rates of preserved and preservative-free topical 8-methoxy fluoroquinolones against various strains of Staphylococcus. Journal of Cataract and Refractive Surgery. 2009;35(9):1609-1613. https://doi.org/10.1016/j.jcrs.2009.04.036
  42. Kosyakova KG, Esaulenko NB, Kameneva OA, Kazakov SP, Dubinina AYu, Mezina EYu, Zaitsev AA. Prevalence of Carbapenemase Genes, qacE, qacEΔ1 and cepA in Multidrug-Resistant Gram-Negative Bacteria with Different Susceptibility to Chlorhexidine. Epidemiology and Vaccinal Prevention. 2020;19(5):49-60. (In Russ.). https://doi.org/10.31631/2073-3046-2020-19-5-49-60
  43. Loughlin MF, Jones MV, Lambert PA. Pseudomonas aeruginosa cells adapted to benzalkonium chloride show resistance to other membrane-active agents but not to clinically relevant antibiotics. The Journal of Antimicrobial Chemotherapy. 2002;49(4):631-639.  https://doi.org/10.1093/jac/49.4.631
  44. McDonnell G, Russell AD. Antiseptics and disinfectants: activity, action and resistance. Clinical Microbiology Reviews. 1999;12(1):147-179.  https://doi.org/10.1128/cmr.12.1.147
  45. Denyer SP. Mechanisms of action of antibacterial biocides. International Biodeterioration Biodegradation. 1995;36:227-245.  https://doi.org/10.1016/0964-8305(96)00015-7
  46. Sheppard FC, Mason DJ, Bloomfield SF, Gant VA. Flow cytometric analysis of chlorhexidine action. FEMS Microbiology Letters. 1997;154(2):283-288.  https://doi.org/10.1111/j.1574-6968.1997.tb12657.x
  47. Chawner J, Gilbert P. Interaction of the bisbiguanidines chlorhexidine and alexidine with phospholipid vesicles: evidence for separate modes of action. The Journal of Applied Bacteriology. 1989;66(3):253-258.  https://doi.org/10.1111/j.1365-2672.1989.tb02476.x
  48. Kholina EG, Kovalenko IB, Bozdaganyan ME, Strakhovskaya MG, Orekhov PS. Cationic Antiseptics Facilitate Pore Formation in Model Bacterial Membranes. The Journal of Physical Chemistry. 2020;124(39):8593-8600. https://doi.org/10.1021/acs.jpcb.0c07212
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