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Alexander-Sinclair E.I.

Institute of Cytology of the Russian Academy of Sciences

Okolov I.N.

Institute of Cytology of the Russian Academy of Sciences

Perepletchikova D.A.

Institute of Cytology of the Russian Academy of Sciences;
Peter the Great St. Petersburg Polytechnic University

Grechanaya Yu.S.

Institute of Cytology of the Russian Academy of Sciences;
Peter the Great St. Petersburg Polytechnic University

Zhurenkov K.E.

Institute of Cytology of the Russian Academy of Sciences;
Saint Petersburg State University

Blinova M.I.

Institute of Cytology of the Russian Academy of Sciences

The in vitro effect of antiseptics on epithelial cells of human cornea and conjunctiva

Authors:

Alexander-Sinclair E.I., Okolov I.N., Perepletchikova D.A., Grechanaya Yu.S., Zhurenkov K.E., Blinova M.I.

More about the authors

Journal: Russian Annals of Ophthalmology. 2021;137(5): 86‑92

DOI: 10.17116/oftalma202113705186

Views: 5461

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To cite this article:

Alexander-Sinclair EI, Okolov IN, Perepletchikova DA, Grechanaya YuS, Zhurenkov KE, Blinova MI. The in vitro effect of antiseptics on epithelial cells of human cornea and conjunctiva. Russian Annals of Ophthalmology. 2021;137(5):86‑92. (In Russ.)
https://doi.org/10.17116/oftalma202113705186

Подписка 2025 (Russian Annals of Ophthalmology)
 
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Effective and safe antiseptic eye preparations are necessary for prevention and treatment of infectious and inflammatory eye diseases.

PURPOSE

in vitro evaluation of the effect of antiseptic eye drops on corneal and conjunctival epithelial cells.

MATERIAL AND METHODS

Antiseptic eye drops «Bactavit», «Vitabact» and «Ocomistin» were the object of the study. Immortalized human corneal epithelial cell lines (HCE) and human conjunctiva (Chang Conjunctiva, Clone 1-5c-4) were used as the test systems. The viability of the cells was assessed by their metabolic activity and morphology using the MTT test and phase-contrast microscopy.

RESULTS

Antiseptic eye drops belonging to different groups of chemical compounds induced cytotoxic effects on the cells of corneal epithelium (HCE) and human conjunctiva (Chang Conjunctiva, Clone 1-5c-4) of varying degrees, leading to morphological and functional changes in those cells.

CONCLUSION

The study confirms the possibility of using cultured cells for the in vitro comparative assessment of the cytotoxic effect of antiseptic ophthalmic agents.

Keywords:

Cell cultures
cytotoxicity
antiseptics
drops

Authors:

Alexander-Sinclair E.I.

Institute of Cytology of the Russian Academy of Sciences

Okolov I.N.

Institute of Cytology of the Russian Academy of Sciences

Perepletchikova D.A.

Institute of Cytology of the Russian Academy of Sciences;
Peter the Great St. Petersburg Polytechnic University

Grechanaya Yu.S.

Institute of Cytology of the Russian Academy of Sciences;
Peter the Great St. Petersburg Polytechnic University

Zhurenkov K.E.

Institute of Cytology of the Russian Academy of Sciences;
Saint Petersburg State University

Blinova M.I.

Institute of Cytology of the Russian Academy of Sciences

Received:

29.04.2021

Accepted:

06.09.2021

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