Histological verification of autofluorescence borders of periorbital skin tumors

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PURPOSE

To compare histological borders of periorbital skin tumors with their autofluorescence borders built from the analysis of non-induced protoporphyrin IX autofluorescence.

MATERIAL AND METHODS

The study group included 8 patients with skin tumors of the eyelids, periorbital region, eyebrow and zygomatic regions aged 54—88 years. The tumors varied in size from 2 to 8 mm and all displayed signs of basal cell carcinoma (BCC). At admission, all the patients underwent non-induced autofluorescence diagnosis. The images were processed with the «CancerPlot» program. During radio excision, the autofluorescent border of each neoplasm was marked with a surgical incision of about 5 mm long and 2 mm deep.

RESULTS

Upon pathomorphological examination, solid BCC was identified in 7 cases. The remaining case was senile keratosis. All reference incisions were located in healthy tissues not farther than 1 mm from the tumor (or keratosis locus, correspondingly).

CONCLUSION

By the example of facial BCC, an evident correlation was established between histological borders of the tumor and its native (non-induced) protoporphyrin IX autofluorescence.

Keywords:

basal cell carcinoma

BCC

tumor borders

fluorescence diagnosis

autofluorescence

protoporphyrin IX

Authors:

Grusha Y.O.

I.M. Sechenov First Moscow State Medical University (Sechenov University);
Krasnov Research Institute of Eye Diseases

ORCID: 0000-0002-6461-8243

Kiryushchenkova N.P.

Research Institute of Eye Diseases

Novikov I.A.

Krasnov Research Institute of Eye Diseases

ORCID: 0000-0003-4898-4662

Fedorov A.A.

Moscow Regional Scientific Research Institute of Obstetrics and Gynecology

ORCID: 0000-0003-2590-5087

Ismailova D.S.

Research Institute of Eye Diseases

Received:

23.04.2020

Accepted:

22.07.2020

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