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Alexandrova O.I.

Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr., 4, St. Petersburg, Russia, 194064

Khorolskaya Y.I.

Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr., 4, St. Petersburg, Russia, 194064

Maĭchuk D.Iu.

FGBU "MNTK "Mikrokhirurgiia glaza" im. akad. S.N. Fedorova" Minzdrava Rossii

Blinova M.I.

Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr., 4, St. Petersburg, Russia, 194064

Study of common cytotoxicity of aminoglycoside and fluoroquinolone antibiotics in cell cultures

Authors:

Alexandrova O.I., Khorolskaya Y.I., Maĭchuk D.Iu., Blinova M.I.

More about the authors

Journal: Russian Annals of Ophthalmology. 2015;131(5): 43‑53

DOI: 10.17116/oftalma2015131543-53

Views: 2289

Downloaded: 113

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To cite this article:

Alexandrova OI, Khorolskaya YI, Maĭchuk DIu, Blinova MI, Alexandrova OI, Khorolskaya YI, Blinova MI. Study of common cytotoxicity of aminoglycoside and fluoroquinolone antibiotics in cell cultures. Russian Annals of Ophthalmology. 2015;131(5):43‑53. (In Russ.)
https://doi.org/10.17116/oftalma2015131543-53

Подписка 2025 (Russian Annals of Ophthalmology)
 
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The aim of the study was to identify the overall cytotoxicity of aminoglycosides netilmicin and tobramycin, and fluoroquinolone ciprofloxacin. Material and methods. We used three types of the cells: constantly transformed cell line CHO-K1, normal human skin fibroblasts and cells of normal human conjunctiva Clone 1-5C-4. Antibiotics activity was detected by their influence on cell viability. Quantitative and qualitative methods of evaluation have used to determine viability of the cells (quantitative assessment: a method of cloning cells and colorimetric method for assessing cell proliferation; qualitative assessment: lifetime visual observation under an inverted microscope for morphological status of cells in culture with photofixing). Results. The most toxic effect for all types of the cells was shown in tobramycin. The least degree of toxicity for all types of the cells was determined in netilmicin. Conclusion. Studied antibiotics exert a cytotoxic effect in vitro and differ in their cytotoxic potential.

Keywords:

cell culture
cytotoxicity
keratocytes
netilmicin
fluoroquinolone
aminoglycoside

Authors:

Alexandrova O.I.

Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr., 4, St. Petersburg, Russia, 194064

Khorolskaya Y.I.

Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr., 4, St. Petersburg, Russia, 194064

Maĭchuk D.Iu.

FGBU "MNTK "Mikrokhirurgiia glaza" im. akad. S.N. Fedorova" Minzdrava Rossii

Blinova M.I.

Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr., 4, St. Petersburg, Russia, 194064

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