Development of selective method for mebeverine detection in blood

Vikman P.S.; Balabanova O.L.; Strelova O.Yu.; Grebenyuk A.N.

doi:https://doi.org/10.17116/sudmed20246701134

Vikman P.S.

Saint Petersburg State Chemical and Pharmaceutical University

ORCID: 0000-0002-5446-8464

Balabanova O.L.

Saint-Petersburg I. I. Dzhanelidze Research Institute of Emergency Medicine

ORCID: 0000-0002-8636-9858

Strelova O.Yu.

Saint Petersburg State Chemical and Pharmaceutical University

ORCID: 0000-0001-6737-1023

Grebenyuk A.N.

Saint Petersburg State Chemical and Pharmaceutical University

ORCID: 0000-0002-9381-194X

Development of selective method for mebeverine detection in blood

Authors:

Vikman P.S., Balabanova O.L., Strelova O.Yu., Grebenyuk A.N.

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Journal: Forensic Medical Expertise. 2024;67(1): 34‑39

DOI: 10.17116/sudmed20246701134

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To cite this article:

Vikman PS, Balabanova OL, Strelova OYu, Grebenyuk AN. Development of selective method for mebeverine detection in blood. Forensic Medical Expertise. 2024;67(1):34‑39. (In Russ.)
https://doi.org/10.17116/sudmed20246701134

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

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Abstract:

OBJECTIVE

To develop the method of biological fluids’ sample processing and mebeverine detection to exclude false results’ receiving when diagnosing drug intoxication.

MATERIAL AND METHODS

The study was carried out using «Mebeverine» (NJCO «North star», Russia) medicine and hydrolysis by enzymes, namely papain, chymotrypsin, trypsin, chymopsin and hyaluronidase, was applied for sample processing. The extractions were analyzed by methods of HPLC-MS/MS on Nexera XR modular liquid chromatograph with LCMS-8050 (Shimadzu) tandem mass spectrometer and GC-MS on gas chromatograph connected with QP-2020 (Shimadzu, Japanese) mono quadrupole mass spectrometer.

RESULTS AND CONCLUSION

It has been revealed that using selective method of sample processing, which consists of aqueous phase extraction at pH=2—4 and enzymatic hydrolysis by papain and hyaluronidase during isolation from blood, is required to detect mebeverine in biological liquids. It has been proven that the native mebeverine is hydrolyzed to veratric (3.4-dimethoxybenzoic) acid and mebeverine alcohol at alkalotic pH value of medium. It has been shown that mebeverine extraction is necessary to study using HPLC-MS/MS, which will allow to avoid the native mebeverine degradation in chromatograph injector as with GC-MS method analysis.

Keywords:

mebeverine

HPLC-MS/MS

false positive result

immunochromatographic method

Authors:

Vikman P.S.

Saint Petersburg State Chemical and Pharmaceutical University

ORCID: 0000-0002-5446-8464

Balabanova O.L.

Saint-Petersburg I. I. Dzhanelidze Research Institute of Emergency Medicine

ORCID: 0000-0002-8636-9858

Strelova O.Yu.

Saint Petersburg State Chemical and Pharmaceutical University

ORCID: 0000-0001-6737-1023

Grebenyuk A.N.

Saint Petersburg State Chemical and Pharmaceutical University

ORCID: 0000-0002-9381-194X

Received:

14.07.2023

Accepted:

20.08.2023

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