Development of selective method for identifying mebeverine in hair

Vikman P.S.; Balabanova O.L.; Strelova O.Yu.; Grebenyuk A.N.

doi:https://doi.org/10.17116/sudmed20246705129

Vikman P.S.

Saint-Petersburg State Chemical and Pharmaceutical University

ORCID: 0000-0002-5446-8464

Balabanova O.L.

Saint-Petersburg I. I. Dzhanelidze Research Institute of Emergency Medicine

ORCID: 0000-0002-8636-9858

Strelova O.Yu.

Saint-Petersburg State Chemical and Pharmaceutical University

ORCID: 0000-0001-6737-1023

Grebenyuk A.N.

Saint-Petersburg State Chemical and Pharmaceutical University

ORCID: 0000-0002-9381-194X

Development of selective method for identifying mebeverine in hair

Authors:

Vikman P.S., Balabanova O.L., Strelova O.Yu., Grebenyuk A.N.

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Journal: Forensic Medical Expertise. 2024;67(5): 29‑35

DOI: 10.17116/sudmed20246705129

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Vikman PS, Balabanova OL, Strelova OYu, Grebenyuk AN. Development of selective method for identifying mebeverine in hair. Forensic Medical Expertise. 2024;67(5):29‑35. (In Russ.)
https://doi.org/10.17116/sudmed20246705129

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

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Abstract:

OBJECTIVE

To develop the method for identification of mebeverine and its metabolites in biological materials (urine and hair) for solution of the problem of possible cross-reaction with psychoactive substances during preliminary studies by immunochromatographic method.

MATERIAL AND METHODS

The study was carried out using «Mebeverine» tablets (NAO «Severnaya Zvezda», Russia), enzymes, namely trypsin, chymotrypsin and hyaluronidase, papain. The obtained extracts were analyzed using HELC-MS/MS on a modular liquid Nexera XR chromatograph with a tandem LCMS-8050 (Shimadzu) mass spectrometer. Guinea pigs weighing about 500 gr were used in modelling the long-term use of mebeverine. The drug was administered per os, once to 5 guinea pigs at a dosage of 200 mg calculated as an animal weighing 500 gr and daily urine was collected. The wool was collected 30 days after regular administration.

RESULTS AND CONCLUSION

The main mebeverine’s metabolites have been determined and native mebeverine and its marker metabolites (mebeverine and veratric acids) have been found in urine while taking this medicine in therapeutic doses for medical purposes. The application of enzymatic hydrolysis of hair method allowed to establish that hair tissue accumulates only a native molecule of mebeverine, therefore, only by results of hair analysis it is possible to conclude, if there was pharmacological use of this medicine or psychoactive substances from the group of phenylalkilamines. The effectiveness of enzymatic hydrolysis by hyaluronidase to isolate mebeverine from hair has been shown.

Keywords:

mebeverine

enzymatic hydrolysis

HELC-MS/MS

hair

metabolites

veratric and mebeverine acids

Authors:

Vikman P.S.

Saint-Petersburg State Chemical and Pharmaceutical University

ORCID: 0000-0002-5446-8464

Balabanova O.L.

Saint-Petersburg I. I. Dzhanelidze Research Institute of Emergency Medicine

ORCID: 0000-0002-8636-9858

Strelova O.Yu.

Saint-Petersburg State Chemical and Pharmaceutical University

ORCID: 0000-0001-6737-1023

Grebenyuk A.N.

Saint-Petersburg State Chemical and Pharmaceutical University

ORCID: 0000-0002-9381-194X

Received:

04.08.2023

Accepted:

27.10.2023

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References:

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