Determination of thiabendazole in the conditions of chemical and toxicological analysis of biomaterial

Shormanov V.K.; Shcherbakov D.P.

doi:https://doi.org/10.17116/sudmed20216403134

Shormanov V.K.

Kursk State Medical University

ORCID: 0000-0001-8872-0691

Shcherbakov D.P.

Kursk State Medical University

Determination of thiabendazole in the conditions of chemical and toxicological analysis of biomaterial

Authors:

Shormanov V.K., Shcherbakov D.P.

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Journal: Forensic Medical Expertise. 2021;64(3): 34‑40

DOI: 10.17116/sudmed20216403134

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To cite this article:

Shormanov VK, Shcherbakov DP. Determination of thiabendazole in the conditions of chemical and toxicological analysis of biomaterial. Forensic Medical Expertise. 2021;64(3):34‑40. (In Russ.)
https://doi.org/10.17116/sudmed20216403134

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

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OBJECTIVE

Is to study the features and to develop the methods for the determination of thiabendazole in the tissues of cadaveric organs and blood. When performing the experiments the methods of TLC, GC-MS and spectrophotometry were used. The advantages were substantiated and the optimal conditions for the release of thiabendazole from the tissues of organs and blood with acetone were determined. A variant of purification of the substance extracted from biomatrixes by the method of column chromatography of normal pressure (sorbent L 40/100 μm, mobile phase acetone-dichloromethane (9.5: 0.5)) is proposed. To identify the analyte by TLC the Sorbfil plates were used and a toluene-acetonitrile mobile phase (2:8). When identifying thiabendazole by a combination of gas-liquid chromatography and mass spectrometry (fragmentation of molecules by electron impact 70 eV) an HP-5MS column 30 m × 0.25 mm with a non-polar stationary phase (5% -phenyl) -methylpolysiloxane was proposed. The expediency of the spectrophotometric determination of thiabendazole on the basis of absorption in an acetonitrile medium has been shown. Methods for the determination of thiabendazole in tissues of organs and blood have been developed and validated. It is shown that the techniques meet the requirements of linearity, selectivity, correctness, precision and stability. The limits of detection of thiabendazole in the liver and blood are 0.14 and 0.10 mg, respectively; the limits of quantitative determination are 0.26 and 0.18 mg per 100 g of biomatrix.

Keywords:

thiabendazole

isolation

purification

identification and determination

Authors:

Shormanov V.K.

Kursk State Medical University

ORCID: 0000-0001-8872-0691

Shcherbakov D.P.

Kursk State Medical University

Received:

26.12.2020

Accepted:

12.01.2021

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