Features of the determination of amlodipine in a biological material

Shormanov V.K.; Kvachahija L.L.; Mitrokhina A.V.; Myasnyankina E.A.

doi:https://doi.org/10.17116/sudmed20196204147

Shormanov V.K.

Kurskiĭ gosudarstvennyĭ meditsinskiĭ universitet

Kvachahija L.L.

GBOU VPO «Kurskij gosudarstvennyj meditsinskij universitet», Kursk, Rossija, 305041

Mitrokhina A.V.

Department of Pharmaceutical, Toxicological and Analytical Chemistry, Kursk State Medical University, Kursk, Russia, 305041

Myasnyankina E.A.

Department of Pharmaceutical, Toxicological and Analytical Chemistry, Kursk State Medical University, Kursk, Russia, 305041

Features of the determination of amlodipine in a biological material

Authors:

Shormanov V.K., Kvachahija L.L., Mitrokhina A.V., Myasnyankina E.A.

More about the authors

Journal: Forensic Medical Expertise. 2019;62(4): 47‑54

DOI: 10.17116/sudmed20196204147

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To cite this article:

Shormanov VK, Kvachahija LL, Mitrokhina AV, Myasnyankina EA. Features of the determination of amlodipine in a biological material. Forensic Medical Expertise. 2019;62(4):47‑54. (In Russ.)
https://doi.org/10.17116/sudmed20196204147

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

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Abstract:

The purpose of the work is to determine the optimal conditions for isolating amlodipine, to purify it by the method of column chromatography and to develop a method for detecting it in biological material. TLC, GC-MS, low pressure column chromatography, and HPLC were used for analysis. We studied the comparative isolation of amlodipine from biological material using 13 isolating agents of organic nature, water, and aqueous solutions. The use of acetone as an insulating agent for the extraction of amlodipine from tissues of cadaver organs has been substantiated. The possibility of purification of the analyzed compound from the endogenous substances of the biomaterial is shown by the method of reversed phase chromatography in a column of the Silasorp S-18 sorbent of 30 μm. A technique has been developed for detecting amlodipine in the tissues of cadaveric organs (liver), which corresponds to the necessary parameters of linearity, selectivity, accuracy, precision and stability. The limits of detection and quantification of amlodipine by the proposed method are 0.25·10-6 and 4.0·10–6 g, respectively, in 1 g of the biomaterial.

Keywords:

amlodipine

isolation

identification and detection

validation

Authors:

Shormanov V.K.

Kurskiĭ gosudarstvennyĭ meditsinskiĭ universitet

Kvachahija L.L.

GBOU VPO «Kurskij gosudarstvennyj meditsinskij universitet», Kursk, Rossija, 305041

Mitrokhina A.V.

Department of Pharmaceutical, Toxicological and Analytical Chemistry, Kursk State Medical University, Kursk, Russia, 305041

Myasnyankina E.A.

Department of Pharmaceutical, Toxicological and Analytical Chemistry, Kursk State Medical University, Kursk, Russia, 305041

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