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Kataev S.S.

Permskoe kraevoe biuro sudebno-meditsinskoĭ ékspertizy

Dvorskaia O.N.

Permskaia gosudarstvennaia farmatsevticheskaia akademiia

Krokhin I.P.

Perm Regional Bureau of Forensic Medical Expertise, Perm, Russia, 614077

Optimization of the solid-phase extraction procedure for the screening of the medicinal and narcotic substances in the blood by gas chromatography with mass-spectrometric detection

Authors:

Kataev S.S., Dvorskaia O.N., Krokhin I.P.

More about the authors

Journal: Forensic Medical Expertise. 2017;60(1): 29‑35

DOI: 10.17116/sudmed201760129-35

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OPTIMIZATION OF THE SOLID-PHASE EXTRACTION PROCEDURE FOR THE SCREENING OF THE MEDICINAL AND NARCOTIC SUBSTANCES IN THE BLOOD BY GAS CHROMATOGRAPHY WITH MASS-SPECTROMETRIC DETECTION
OPTIMIZATION OF THE SOLID-PHASE EXTRACTION PROCEDURE FOR THE SCREENING OF THE MEDICINAL AND NARCOTIC SUBSTANCES IN THE BLOOD BY GAS CHROMATOGRAPHY WITH MASS-SPECTROMETRIC DETECTION

To cite this article:

Kataev SS, Dvorskaia ON, Krokhin IP. Optimization of the solid-phase extraction procedure for the screening of the medicinal and narcotic substances in the blood by gas chromatography with mass-spectrometric detection. Forensic Medical Expertise. 2017;60(1):29‑35. (In Russ.)
https://doi.org/10.17116/sudmed201760129-35

Подписка 2025-2 (Forensic Medical Expertise)
 
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This paper was designed to describe the application of the method for solid-phase extraction of the medicinal and narcotic substances having different physicochemical composition by gas chromatography with mass-spectrometric detection (GC-MS) for the purpose of their screening in the blood. The solid-phase extraction technique was optimized by means of the Box—Behnken modeling with the evaluation of the influence on the effectiveness of extraction of various factors including pH of the buffer solution, eluent composition, the type and the volume of the solutions used to wash the sorbent.

Keywords:

blood
solid-phase extraction
Box—Behnken modeling
gas chromatography/mas-spectrometry

Authors:

Kataev S.S.

Permskoe kraevoe biuro sudebno-meditsinskoĭ ékspertizy

Dvorskaia O.N.

Permskaia gosudarstvennaia farmatsevticheskaia akademiia

Krokhin I.P.

Perm Regional Bureau of Forensic Medical Expertise, Perm, Russia, 614077

List of references:

  1. Drummer OH. Chromatographic screening techniques in systematic toxicological analysis. Journal of Chromatography B. 1999;733:27-45. doi:10.1016/S0378-4347(99)00265-0
  2. Xiao-Hua Chen, Jan-Piet Franke, Jaap Wijsbeek, Rokus A. de Zeeuw. Isolation of Acidic, Neutral, and Basic Drugs from Whole Blood Using A Single Mixed-Mode Solid-Phase Extraction Column. Journal of Analytical Toxicology. 1992;16(6):351-355. doi:10.1093/jat/16.6.351
  3. Xiao-Hua Chen, Jan-Piet Franke, Jaap Wijsbeek, Rokus A. de Zeeuw. Study of lot-to-lot reproducibilities of Bond Elut Certify and Clean Screen DAU mixed-mode solid-phase extraction columns in the extraction of drugs from whole blood. Journal of Chromatography Biomedical Applications. 1993;617(1):147-151. doi:10.1016/0378-4347(93)80434-6
  4. Chi-Kong Lai, Ting Lee, Kam-Ming Au, Albert Yan-Wo Chan. Uniform solid-phase extraction procedure for toxicological drug screening in serum and urine by HPLC with photodiode-array detection. Clinical Chemistry. 1997;43(2):312-325.
  5. Rittner M, Pragst F, Bork WR, Neumann J. Screening Method for Seventy Psychoactive Drugs or Drug Metabolites in Serum Based on High-Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry. Journal of Analytical Toxicology. 2001;25(2):115-124. doi:10.1093/jat/25.2.115
  6. Takeda A, Tanaka H, Shinohara T, Ohtake I. Systematic analysis of acid, neutral and basic drugs in horse plasma by combination of solid-phase extraction, non-aqueous partitioning and gas chromatography-mass spectrometry. Journal of Chromatography B. 2001;758:235-248. doi:10.1016/s0378-4347(01)00189-x
  7. Kataev SS, Dvorskaya ON. The application of the solid phase extraction technique to the analysis of blood for the presence of narcotic and pharmaceutical substances. Sudebno-meditsinskaya ekspertiza. 2012;55(4):38-42. (In Russ.).
  8. Krylova EA, Kataev SS, Dvorskaya ON, Khomov YuA. Application of solid phase extraction for co-extraction of zolpidem and its metabolites from urine. Butlerov Communications.2013;35(9):61-70. (In Russ.).
  9. Dvorskaya ON, Kataev SS, Krokhin IP. Identification several of NSAIDs in blood screening by solid-phase extraction and gas chromatography-mass spectrometry. Butlerov Communications. 2015;43(7):149-153. (In Russ.).
  10. Piñero EL, Casale JF. Quantitation of Cocaine by Gas Chromatography-Flame Ionization Detection Utilizing Isopropylcocaine as a Structurally Related Internal Standard. Microgram Journal. 2006;4(1-4):47-53.
  11. Lukyanova NYu. Statistics: Correlation and regression analysis statistical relationships on a personal computer. Kaliningrad: Kaliningrad University. 1999. (In Russ.). http://www.aup.ru/books/m1021/

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