Cytocompatibility of hydroxyapatite coating for titanium implants, applied by radio-frequency magnetron sputtering at low pressures in an inert gas atmosphere

Basina V.P.; Zadiriev I.I.; Mironov A.V.; Vasilyev A.V.; Nechay A.E.; Goldstein D.V.

doi:https://doi.org/10.17116/stomat202510406232

Basina V.P.

Research Centre for Medical Genetics

ORCID: 0009-0006-0127-6502

Zadiriev I.I.

Lomonosov Moscow State University

ORCID: 0000-0003-3824-2016

Mironov A.V.

National Research Center “Kurchatov Institute”

SPIN RSCI: 7185-9165
Scopus AuthorID: 3559168 0400
ResearcherID: F-1555-2014
ORCID: 0000-0002-8173-0253

Vasilyev A.V.

Research Centre for Medical Genetics;
Central Research Institute of Dentistry and Maxillofacial Surgery

SPIN RSCI: 8864-6279
Scopus AuthorID: 56865840500
ResearcherID: C-9338-2013
ORCID: 0000-0002-7169-2724

Nechay A.E.

Plastrek Co.

ORCID: 0009-0008-1747-2166

Goldstein D.V.

Research Centre for Medical Genetics

ORCID: 0000-0003-2438-1605

Cytocompatibility of hydroxyapatite coating for titanium implants, applied by radio-frequency magnetron sputtering at low pressures in an inert gas atmosphere

Authors:

Basina V.P., Zadiriev I.I., Mironov A.V., Vasilyev A.V., Nechay A.E., Goldstein D.V.

More about the authors

Journal: Stomatology. 2025;104(6‑2): 32‑37

DOI: 10.17116/stomat202510406232

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To cite this article:

Basina VP, Zadiriev II, Mironov AV, Vasilyev AV, Nechay AE, Goldstein DV. Cytocompatibility of hydroxyapatite coating for titanium implants, applied by radio-frequency magnetron sputtering at low pressures in an inert gas atmosphere. Stomatology. 2025;104(6‑2):32‑37. (In Russ.)
https://doi.org/10.17116/stomat202510406232

Подписка 2026 Стоматология (Stomatology)

Использование инфографики авторами научных работ

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OBJECTIVE

Study of cytocompatibility of hydroxyapatite coating for titanium implants applied by high-frequency magnetron sputtering at low pressures in an inert gas atmosphere.

MATERIALS AND METHODS

Hydroxyapatite was deposited on titanium substrates using the HF magnetron sputtering method. The cytocompatibility of the materials was assessed by incubating with multipotent mesenchymal stromal cells (MSCs) using the MTT assay. Adhesion properties were studied by staining cells with fluorescent dyes and using scanning electron microscopy.

RESULTS

The studied titanium materials possess high cytocompatibility. The number of living cells on the 7th day was 100.0±5.6% and 108.5±8.7% for materials with and without coating, respectively. Furthermore, coating titanium substrates with hydroxyapatite resulted in a statistically significant 1.7±0.4-fold increase in the number of adhered MSCs compared to uncoated materials.

CONCLUSION

The hydroxyapatite coating applied using HF magnetron sputtering significantly improves cell adhesion to titanium materials. Thus, the obtained results provide a basis for the development of new implants with functionalized surfaces.

Keywords:

hydroxyapatite

radio-frequency magnetron sputtering at low pressures in an inert gas atmosphere

bone tissue engineering

Authors:

Basina V.P.

Research Centre for Medical Genetics

ORCID: 0009-0006-0127-6502

Zadiriev I.I.

Lomonosov Moscow State University

ORCID: 0000-0003-3824-2016

Mironov A.V.

National Research Center “Kurchatov Institute”

SPIN RSCI: 7185-9165
Scopus AuthorID: 3559168 0400
ResearcherID: F-1555-2014
ORCID: 0000-0002-8173-0253

Vasilyev A.V.

Research Centre for Medical Genetics;
Central Research Institute of Dentistry and Maxillofacial Surgery

SPIN RSCI: 8864-6279
Scopus AuthorID: 56865840500
ResearcherID: C-9338-2013
ORCID: 0000-0002-7169-2724

Nechay A.E.

Plastrek Co.

ORCID: 0009-0008-1747-2166

Goldstein D.V.

Research Centre for Medical Genetics

ORCID: 0000-0003-2438-1605

Received:

30.09.2025

Accepted:

01.10.2025

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References:

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