The site of the Media Sphera Publishers contains materials intended solely for healthcare professionals.
By closing this message, you confirm that you are a certified medical professional or a student of a medical educational institution.

Login
EN
+7 (495) 482-4118
+7 (495) 482-0604
+8 800 250-18-12
  • (toll-free number for subscriptions)
    Mon-Fri from 10 a.m. to 6 p.m.

  • © 1998-2025
+7 (495) 482-43-29
EN
All journals
Journal
Year
Year
Search result: 0

Blinova A.V.

Tver State Medical University

Rumyantsev V.A.

Tver State Medical University

Bityukova E.V.

Tver State Medical University

Rodionova E.G.

Tver State Medical University

Bessudnova A.R.

Tver State Medical University

Evaluation of the antibacterial effect of passive nanoimpregnation of tooth root dentin by real-time PCR

Authors:

Blinova A.V., Rumyantsev V.A., Bityukova E.V., Rodionova E.G., Bessudnova A.R.

More about the authors

Journal: Stomatology. 2023;102(3): 11‑15

DOI: 10.17116/stomat202310203111

Read: 918 times

Download PDF (RU)
Contact the author
Table of contents

EVALUATION OF THE ANTIBACTERIAL EFFECT OF PASSIVE NANOIMPREGNATION OF TOOTH ROOT DENTIN BY REAL-TIME PCR
EVALUATION OF THE ANTIBACTERIAL EFFECT OF PASSIVE NANOIMPREGNATION OF TOOTH ROOT DENTIN BY REAL-TIME PCR

To cite this article:

Blinova AV, Rumyantsev VA, Bityukova EV, Rodionova EG, Bessudnova AR. Evaluation of the antibacterial effect of passive nanoimpregnation of tooth root dentin by real-time PCR. Stomatology. 2023;102(3):11‑15. (In Russ.)
https://doi.org/10.17116/stomat202310203111

Подписка 2025-2 (Stomatology)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
Mediasphera_Net
Close metadata
Recommended articles:
Study of wound healing effect of silver and cerium nano­particles in treatment of unco­mplicated skin wounds in an expe­riment. Russian Journal of Operative Surgery and Clinical Anatomy. 2024;(4):18-24

OBJECTIVE

Assessment of antibacterial properties of a new intracanal paste based on calcium hydroxocuprate (CHC) and silver nanoparticles hydrosol for passive root impregnation.

MATERIAL AND METHODS

The study included 55 teeth with 69 root canals belonging patients with chronic apical periodontitis. The main group, including 44 root canals, was filled with a new paste based on CHC and silver nanoparticles for 7 days after preparation and irrigation. In the control group, 25 root canals were sealed with an aqueous paste of calcium hydroxide for 14 days. The presence of the endodontic microorganisms was evaluated by real-time PCR.

RESULTS AND DISCUSSION

Further analysis showed that the amount of the DNA, common for P. gingivalis, T. forsythia and T. denticola, after treatment was less in the main group, where the new paste was applied. These results were significant at the p=0.05 level (p=0.005, p=0.006, p=0.003 according to each mentioned bacterial sample). No significant differences were found between the groups in the number of genome equivalents specific for P. intermedia and F. nucleatum (p=0.543, p=0.554).

CONCLUSION

These findings suggest that the new method of passive root impregnation with the CHC and silver nanoparticles paste may be an effective method for the treatment of chronical apical periodontitis.

Keywords:

apical periodontitis
microbial biofilm
medical treatment of root canals
silver nanoparticles

Authors:

Blinova A.V.

Tver State Medical University

Rumyantsev V.A.

Tver State Medical University

Bityukova E.V.

Tver State Medical University

Rodionova E.G.

Tver State Medical University

Bessudnova A.R.

Tver State Medical University

Received:

10.10.2022

Accepted:

27.02.2023

List of references:

  1. Signor B, Blomberg LC, Kopper PMP, Augustin PAN, Rabber MV, Rodrigues GS, Scarparo RK. Root canal retreatment: a retrospective investigation using regression and data mining methods for the prediction of technical quality and periapical healing. J Appl Oral Sci. 2021;29:1-14.  https://doi.org/10.1590/1678-7757-2020-0799
  2. Liu SQ, Chen X, Wang XX, Liu W, Zhou X, Wang X. Outcomes and prognostic factors of apical periodontitis by root canal treatment and endodontic microsurgery — a retrospective cohort study. Ann Palliat Med. 2021;10(5): 5027-5045. https://doi.org/10.21037/apm-20-2507
  3. Chepurova NI, Romanenko IG. The use of calcium copper hydroxide depophoresis in the treatment of chronic periodontitis with impenetrable root canals. Vestnik fisioterapii i kurortologii. 2018;24(2):120-120. (In Russ.).
  4. Deryabina LV, Smirnova LV, Deryabin PM, Moroz TB. Features of use depoforez of hydrooxigen copper-calcium in chronic apical periodontitis. Endodontiya Today. 2014;12(3):68-71. (In Russ.).
  5. Rumyantsev VA, Fedotova TA, Yusupova YuI, Ryabikov MD, Zadorozhnyi DV. Efficiency of endodontic nano-impregnation and curettage with kupral in complex treatment of endodontic and periodontal syndrome. Mezhdunarodnyj nauchno-issledovatel’skij zhurnal. 2017;5(10-2):44-48. (In Russ.). https://doi.org/10.23670/IRJ.2017.64.058
  6. Rumyantsev VA, Frolov GA, Blinova AV, Karasenkov YaN, Bityukova EV. Electron microscopic properties of a new antimicrobial nanodrug based on copper-calcium hydroxide compound. Vestnik Avitsenny. 2021;23(4):532-541. (In Russ.). https://doi.org/10.25005/2074-0581-2021-23-4-532-541
  7. Hargreaves KM, Berman LH, Rothstein I. Cohen’s Pathways of the Pulp. 11th edition. M.: GEOTAR-Media; 2020.
  8. Bergenholtz G, Horsted-Bindslev P, Reit C. Textbook of endodontology. Second edition. Blackwell Publishing Ltd; 2010.
  9. Siqueira JF Jr, Rôças IN. Treponema species associated with abscesses of endodontic origin. Oral Microbiol Immunol. 2004;19(5):336-339.  https://doi.org/10.1111/j.1399-302x.2004.00156.x
  10. Nelson-Filho P, Ruviére DB, de Queiroz AM, de Paula-Silva FWG, da Silva RAB, Lucisano MP, da Silva LAB. Comparative Molecular Analysis of Gram-Negative Bacteria in Primary Teeth with Irreversible Pulpitis or Periapical Pathology. Pediatr Dent. 2018;40(4):259-264. 
  11. Singh KK, Kumar P, Das P, Morandi M, Panda S, Mahajan A, Kumar D. Association of specific microorganisms with endodontic signs and symptoms. A comparative study. J Family Med Prim Care. 2020;9(8):3965-3969. https://doi.org/10.4103/jfmpc.jfmpc_523_20
  12. Gomez BPFA, Pinheiro ET, Jacinto RC, Zaia AA, Ferraz CCR, Francisco Souza-Filho FJ. Microbial analysis of canals of root-filled teeth with periapical lesions using polymerase chain reaction. J Endod. 2008;34(5):537-540.  https://doi.org/10.1016/j.joen.2008.01.016
  13. Foschi F, Cavrini F, Montebugnoli L, Steshenko P, Sambri V, Prati C. Detection of bacteria in endodontic samples by polymerase chain reaction assays and association with defined clinical signs in Italian patients. Oral Microbiol Immunol. 2005;20(5):289-295.  https://doi.org/10.1111/j.1399-302X.2005.00227.x

Close metadata

Contact the author
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.

Email Confirmation

An email was sent to test@gmail.com with a confirmation link. Follow the link from the letter to complete the registration on the site.

Email Confirmation

Contact us
If you have any questions, complaints or suggestions, please use this feedback form.
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.
Submit Application

You can become an author of one of our magazines, send a request and we will consider it in during the day.

Name
Phone
E-mail
Message
Login
Registration
E-mail
Password
Forgot Password?

Log in to the site using your account in one of the services

Password recovery
E-mail
Login
Register

We use cооkies to improve the performance of the site. By staying on our site, you agree to the terms of use of cооkies. To view our Privacy and Cookie Policy, please. click here.