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Blinova A.V.

Tver State Medical University

Rumyantsev V.A.

Tver State Medical University

Bityukova E.V.

Tver State Medical University

Rodionova E.G.

Tver State Medical University

Bessudnova A.R.

Tver State Medical University

Evaluation of the antibacterial effect of passive nanoimpregnation of tooth root dentin by real-time PCR

Authors:

Blinova A.V., Rumyantsev V.A., Bityukova E.V., Rodionova E.G., Bessudnova A.R.

More about the authors

Journal: Stomatology. 2023;102(3): 11‑15

DOI: 10.17116/stomat202310203111

Read: 1140 times

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Table of contents

EVALUATION OF THE ANTIBACTERIAL EFFECT OF PASSIVE NANOIMPREGNATION OF TOOTH ROOT DENTIN BY REAL-TIME PCR
EVALUATION OF THE ANTIBACTERIAL EFFECT OF PASSIVE NANOIMPREGNATION OF TOOTH ROOT DENTIN BY REAL-TIME PCR

To cite this article:

Blinova AV, Rumyantsev VA, Bityukova EV, Rodionova EG, Bessudnova AR. Evaluation of the antibacterial effect of passive nanoimpregnation of tooth root dentin by real-time PCR. Stomatology. 2023;102(3):11‑15. (In Russ.)
https://doi.org/10.17116/stomat202310203111

 
Подписка 2026 Стоматология (Stomatology)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

OBJECTIVE

Assessment of antibacterial properties of a new intracanal paste based on calcium hydroxocuprate (CHC) and silver nanoparticles hydrosol for passive root impregnation.

MATERIAL AND METHODS

The study included 55 teeth with 69 root canals belonging patients with chronic apical periodontitis. The main group, including 44 root canals, was filled with a new paste based on CHC and silver nanoparticles for 7 days after preparation and irrigation. In the control group, 25 root canals were sealed with an aqueous paste of calcium hydroxide for 14 days. The presence of the endodontic microorganisms was evaluated by real-time PCR.

RESULTS AND DISCUSSION

Further analysis showed that the amount of the DNA, common for P. gingivalis, T. forsythia and T. denticola, after treatment was less in the main group, where the new paste was applied. These results were significant at the p=0.05 level (p=0.005, p=0.006, p=0.003 according to each mentioned bacterial sample). No significant differences were found between the groups in the number of genome equivalents specific for P. intermedia and F. nucleatum (p=0.543, p=0.554).

CONCLUSION

These findings suggest that the new method of passive root impregnation with the CHC and silver nanoparticles paste may be an effective method for the treatment of chronical apical periodontitis.

Keywords:

apical periodontitis
microbial biofilm
medical treatment of root canals
silver nanoparticles

Authors:

Blinova A.V.

Tver State Medical University

Rumyantsev V.A.

Tver State Medical University

Bityukova E.V.

Tver State Medical University

Rodionova E.G.

Tver State Medical University

Bessudnova A.R.

Tver State Medical University

Received:

10.10.2022

Accepted:

27.02.2023

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References:

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  2. Liu SQ, Chen X, Wang XX, Liu W, Zhou X, Wang X. Outcomes and prognostic factors of apical periodontitis by root canal treatment and endodontic microsurgery — a retrospective cohort study. Ann Palliat Med. 2021;10(5): 5027-5045. https://doi.org/10.21037/apm-20-2507
  3. Chepurova NI, Romanenko IG. The use of calcium copper hydroxide depophoresis in the treatment of chronic periodontitis with impenetrable root canals. Vestnik fisioterapii i kurortologii. 2018;24(2):120-120. (In Russ.).
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  5. Rumyantsev VA, Fedotova TA, Yusupova YuI, Ryabikov MD, Zadorozhnyi DV. Efficiency of endodontic nano-impregnation and curettage with kupral in complex treatment of endodontic and periodontal syndrome. Mezhdunarodnyj nauchno-issledovatel’skij zhurnal. 2017;5(10-2):44-48. (In Russ.). https://doi.org/10.23670/IRJ.2017.64.058
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