Study of wound healing effect of silver and cerium nanoparticles in treatment of uncomplicated skin wounds in an experiment

Zinchenko IA, Struts IS, Mirontsev AV, et al. . Study of wound healing effect of silver and cerium nanoparticles in treatment of uncomplicated skin wounds in an experiment. Russian Journal of Operative Surgery and Clinical Anatomy. 2024;8(4):18‑24. (In Russ.)
https://doi.org/10.17116/operhirurg2024804118

Подписка 2025-2 (Russian Journal of Operative Surgery and Clinical Anatomy)

Close metadata

OBJECTIVE

Evaluation of the effectiveness of wound healing of uncomplicated flat wounds against the background of topical application of cerium and silver nanoparticles in a spray in an experiment on laboratory rats.

MATERIAL AND METHODS

The experimental study was performed on 40 Wistar rats, which were modeled with a layered skin wound with an area of 225 mm². The animals were randomly divided into 4 groups. The spray with the studied nanoparticles and the comparison drug dexpanthenol were applied to the wound once for 7 days. On days 3, 7, 14, 21, the wound area was measured to determine the rate of wound healing. The animals were withdrawn from the experiment on days 7, 14, 21 and 30 under general anesthesia, followed by wound tissue sampling for morphological examination. On day 30, the cosmetic effect of the postoperative scar was assessed using the SCAR scale.

RESULTS

Topical application of a spray containing silver (NS) and cerium (NC) nanoparticles reduces the time of complete healing of skin defects by 8±0.5 days (p<0.05), accelerates the wound healing process compared to the control group. On the 7^th day, the average wound healing rate was: in group K — 20.5±2.5 mm²/day; in group S — 29.3±1.8 mm²/day; in group C — 30.2±1.8 mm²/day; in group D — 21.3±9.8 mm²/day (p<0.05). The use of NS and NC improves the cosmetic characteristics of the scar, assessed on the SCAR scale: in group K — 5 points, in group S — 1 point, in group C — 1 point, in group D — 2 points.

CONCLUSION

The study demonstrated the effectiveness of NS and NC in the treatment of uncomplicated skin wounds, characterized by accelerated wound healing, antimicrobial and anti-inflammatory effects, formation of a scar with a smaller surface area and a more favorable cosmetic effect.

Keywords:

wound

scar

silver nanoparticles

cerium nanoparticles

Authors:

Zinchenko I.A.

Sechenov First Moscow State Medical University (Sechenov University)

ORCID: 0009-0007-6499-5659

Struts I.S.

Sechenov First Moscow State Medical University (Sechenov University)

ORCID: 0009-0001-9443-1244

Mirontsev A.V.

Sechenov First Moscow State Medical University (Sechenov University);
National Research Nuclear University

ORCID: 0000-0003-1783-694X

Klimentov S.M.

National Research Nuclear University

ORCID: 0000-0002-3632-4096

Dydykin S.S.

Sechenov First Moscow State Medical University (Sechenov University)

ORCID: 0000-0002-1273-0356

Sorokvasha I.N.

National Research Nuclear University

ORCID: 0000-0002-8314-6616

Romanov M.D.

Ogarev State University

ORCID: 0000-0002-9646-4007

Eldyreva M.V.

Ulyanov State University

ORCID: 0000-0003-2076-9477

Blinova E.V.

Sechenov First Moscow State Medical University (Sechenov University);
National Research Nuclear University

ORCID: 0000-0003-0050-0251

Timoshkin S.P.

National Research Nuclear University

ORCID: 0000-0001-5518-6859

Received:

14.09.2024

Accepted:

03.10.2024

List of references:

Muromtseva EV, Sergatskiy KI, Nikolskiy VI, Shabrov AV, Aldzhabr M, Zakharov AD. Wound treatment depending on the phase of the wound process. Izvestiya vysshikh uchebnykh zavedenii. Povolzhskii region. Meditsinskie nauki. 2022;63(3):93-109. (In Russ.). https://doi.org/10.21685/2072-3032-2022-3-9
Morozov AM., Sergeyev AN, Sergeev NA, Dubatolov GA, Ryzhova TS, Pakhomov MA, Peltikhina OV. Modern methods of stimulating process of postoperative wounds regeneration. Sibirskoe meditsinskoe obozrenie. 2020;123(3):54-60. (In Russ.). https://doi.org/10.20333/2500136-2020-3-54-60
Tamrazova OB. Reparative therapy of “small” wounds from the position of dermatologist and cosmetologist. Allergologiya i immunologiya v pediatrii. 2019;57(2):4-12. (In Russ.).
Kudryashova IS, Markov PA, Kostromina EY, Eremin PS, Rachin AP, Gilmutdinova IR. Development of Wound Dressing for Regenerative Medicine. Vestnik vosstanovitel’noi meditsiny. 2021;20(6):84-95. (In Russ.).] https://doi.org/10.38025/2078-1962-2021-20-6-84-95
Kolimi P, Narala S, Nyavanandi D, Youssef AAA, Dudhipala N. Innovative Treatment Strategies to Accelerate Wound Healing: Trajectory and Recent Advancements. Cells. 2022;11(15):2439. https://doi.org/10.3390/cells11152439
Freedman BR, Hwang C, Talbot S, Hibler B, Matoori S, Mooney DJ. Breakthrough treatments for accelerated wound healing. Sci Adv. 2023;9(20):eade7007. https://doi.org/10.1126/sciadv.ade7007
Galichenko KA, Sukhov AV, Timoshkin SP, Alkhatatnekh BAS, Mironov MM, Eldyreva MV, Sorokvasha IV, Blinova EV. Experimental study of topical application of cerium oxide nanoparticles on tissue regeneration. Mediko-farmatsevticheskii zhurnal “Pul’s”. 2023;25(5): 96-100. (In Russ.). https://doi.org/10.26787/nydha-2686-6838-2023-25-5-96-100
Korolev DV, Shumatov VB, Plekhova NG. Local treatment of infected wounds depending on the phase of the wound process. Mediko-farmatsevticheskii zhurnal “Pul’s”. 2023;25(7):69-75. (In Russ.). https://doi.org/10.26787/nydha-2686-6838-2023-25-7-69-75
Udegova ES, Gildeeva KA, Rukosueva TV, Baker S. Metal nanoparticle antibacterial effect on antibiotic-resistant strains of bacteria. Infektsiya i immunitet. 2021;11(4):771-776. (In Russ.). https://doi.org/10.15789/2220-7619-MNA-1359
Wahab S, Salman A, Khan Z, Khan S, Krishnaraj C, Yun SI. Metallic Nanoparticles: A Promising Arsenal against Antimicrobial Resistance-Unraveling Mechanisms and Enhancing Medication Efficacy. Int J Mol Sci. 2023;24(19):14897. https://doi.org/10.3390/ijms241914897
Sheikh-Oleslami, S, Tao, B, D’Souza, J, Butt, F, Suntharalingam, H, Rempel, L, Amiri, N. A Review of Metal Nanoparticles Embedded in Hydrogel Scaffolds for Wound Healing In Vivo. Gels. 2023;9(7):591. https://doi.org/10.3390/gels9070591
Olteanu G, Neacșu SM, Joița FA, et al. Advancements in Regenerative Hydrogels in Skin Wound Treatment: A Comprehensive Review. Int J Mol Sci. 2024;25(7):3849. https://doi.org/10.3390/ijms25073849
Shekhter AB, Pekshev AV, Vagapov AB, Butenko AV, Fayzullin AL, Rudenko TG, Sharapov NA, Serejnikova NB, Vasilets VN. Dose-dependent effect of plasma-chemical NO-containing gas flow on wound healing. An experimental study. Clin Plasma Med. 2020;19-20:100101. https://doi.org/10.1016/j.cpme.2020.100101
Galichenko KA, Blinova EV, Simakina EA, Suhov AV, Shimanovskij DN, Gilevskaja JuS, Skachilova SJa, Timoshkin SP, Kyt’ko OV, Sorokvasha IN, Bogojavlenskaja TA. Study of the healing processes of postoperative wounds during Z-shaped skin plastic surgery in an experiment using the cerium-containing compound N-acetyl-6-aminohexanoic acid. Russian Journal of Operative Surgery and Clinical Anatomy (Pirogov scientific journal). 2022;6(3):5-11. (In Russ.). https://doi.org/10.17116/operhirurg202260315
Zheng Q, Chen C, Liu Y, Gao J, Li L, Yin C, Yuan X. Metal Nanoparticles: Advanced and Promising Technology in Diabetic Wound Therapy. Int J Nanomedicine. 2024;19:965-992. https://doi.org/10.2147/IJN.S434693

Close metadata