OBJECTIVE
Evaluation of the effectiveness of wound healing of uncomplicated flat wounds against the background of topical application of cerium and silver nanoparticles in a spray in an experiment on laboratory rats.
MATERIAL AND METHODS
The experimental study was performed on 40 Wistar rats, which were modeled with a layered skin wound with an area of 225 mm2. The animals were randomly divided into 4 groups. The spray with the studied nanoparticles and the comparison drug dexpanthenol were applied to the wound once for 7 days. On days 3, 7, 14, 21, the wound area was measured to determine the rate of wound healing. The animals were withdrawn from the experiment on days 7, 14, 21 and 30 under general anesthesia, followed by wound tissue sampling for morphological examination. On day 30, the cosmetic effect of the postoperative scar was assessed using the SCAR scale.
RESULTS
Topical application of a spray containing silver (NS) and cerium (NC) nanoparticles reduces the time of complete healing of skin defects by 8±0.5 days (p<0.05), accelerates the wound healing process compared to the control group. On the 7th day, the average wound healing rate was: in group K — 20.5±2.5 mm2/day; in group S — 29.3±1.8 mm2/day; in group C — 30.2±1.8 mm2/day; in group D — 21.3±9.8 mm2/day (p<0.05). The use of NS and NC improves the cosmetic characteristics of the scar, assessed on the SCAR scale: in group K — 5 points, in group S — 1 point, in group C — 1 point, in group D — 2 points.
CONCLUSION
The study demonstrated the effectiveness of NS and NC in the treatment of uncomplicated skin wounds, characterized by accelerated wound healing, antimicrobial and anti-inflammatory effects, formation of a scar with a smaller surface area and a more favorable cosmetic effect.