Comparative analysis of methods to stimulate and collect dentinal fluid: a clinical and experimental study

Mitronin A.V.; Mitronin Yu.A.; Ostanina D.A.; Ostrovskaya I.G.; Mitronin V.A.; Wolgin M.

doi:https://doi.org/10.17116/stomat20211000617

Mitronin A.V.

A.I. Evdokimov Moscow State Medical-Dental University

Mitronin Yu.A.

A.I. Yevdokimov Moscow State University of Medicine and Dentistry

Ostanina D.A.

A.I. Yevdokimov Moscow State University of Medicine and Dentistry

Ostrovskaya I.G.

A.I. Yevdokimov Moscow State University of Medicine and Dentistry

Mitronin V.A.

A.I. Yevdokimov Moscow State University of Medicine and Dentistry

Wolgin M.

Danube Private University

Comparative analysis of methods to stimulate and collect dentinal fluid: a clinical and experimental study

Authors:

Mitronin A.V., Mitronin Yu.A., Ostanina D.A., Ostrovskaya I.G., Mitronin V.A., Wolgin M.

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Journal: Stomatology. 2021;100(6): 7‑12

DOI: 10.17116/stomat20211000617

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To cite this article:

Mitronin AV, Mitronin YuA, Ostanina DA, Ostrovskaya IG, Mitronin VA, Wolgin M. Comparative analysis of methods to stimulate and collect dentinal fluid: a clinical and experimental study. Stomatology. 2021;100(6):7‑12. (In Russ.)
https://doi.org/10.17116/stomat20211000617

Подписка 2026 Стоматология (Stomatology)

Использование инфографики авторами научных работ

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OBJECTIVE

The aim — to determine the most effective method of dentinal fluid collection and dentinal fluid flow rate stimulation.

MATERIAL AND METHODS

In a series of dentinal fluid collection tests, 3 types of filter membranes «Technofilter» were compared to a blotting nitrocellulose membrane Sartorius 1288. An ability of the studied membranes to adsorb dentinal fluid was assessed based on the data of proton magnetic resonance using signal magnitudes of the organic molecules accumulation in dentine fluid samples. In the study 30 permanent teeth with a diagnosis of K04.00 «Initial pulpitis» were included. Teeth were randomly divided into 5 groups in accordance with the method used to stimulate the dentinal fluid flow rate. Dentinal fluid volume before and after stimulation within and between the groups was compared.

RESULTS

The nitrocellulose membrane Sartorius 1288 has the highest ability to adsorb dentinal fluid; predominant dentinal fluid volume was obtained using a 10% dextran solution with low molecular weight. Based on the results of the study, the author proposed the method of dentinal fluid collection.

CONCLUSION

The proposed stimulation method increases the dentinal fluid yields by 2 times in comparison with sampling under natural conditions.

Keywords:

dentinal fluid

pulp diagnostics

reversible pulpitis

Authors:

Mitronin A.V.

A.I. Evdokimov Moscow State Medical-Dental University

Mitronin Yu.A.

A.I. Yevdokimov Moscow State University of Medicine and Dentistry

Ostanina D.A.

A.I. Yevdokimov Moscow State University of Medicine and Dentistry

Ostrovskaya I.G.

A.I. Yevdokimov Moscow State University of Medicine and Dentistry

Mitronin V.A.

A.I. Yevdokimov Moscow State University of Medicine and Dentistry

Wolgin M.

Danube Private University

Received:

15.12.2020

Accepted:

21.06.2021

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References:

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