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Astashina N.B.

Perm State Medical University named after academician E.A. Wagner of the Ministry of Healthcare of the Russian Federation

Godovalov A.P.

Perm State Medical University named after academician E.A. Wagner of the Ministry of Healthcare of the Russian Federation

Rogozhnikova E.P.

Perm State Medical University named after academician E.A. Wagner of the Ministry of Healthcare of the Russian Federation

Tsareva T.V.

Moscow State University of Medicine and Dentistry

Trefilova Yu.A.

A.I. Yevdokimov Moscow State University of Medicine and Dentistry of the Ministry of Healthcare of the Russian Federation

Grachev D.I.

A.I. Yevdokimov Moscow State University of Medicine and Dentistry of the Ministry of Healthcare of the Russian Federation

Arutyunov A.S.

Central Research Institute of Dentistry and Maxillofacial Surgery

Colonization activity of conditionally pathogenic microorganisms and features of biofilm formation on the surface of thermoplastic polymer material

Authors:

Astashina N.B., Godovalov A.P., Rogozhnikova E.P., Tsareva T.V., Trefilova Yu.A., Grachev D.I., Arutyunov A.S.

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Journal: Stomatology. 2021;100(4): 72‑76

DOI: 10.17116/stomat202110004172

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Table of contents

COLONIZATION ACTIVITY OF CONDITIONALLY PATHOGENIC MICROORGANISMS AND FEATURES OF BIOFILM FORMATION ON THE SURFACE OF THERMOPLASTIC POLYMER MATERIAL
COLONIZATION ACTIVITY OF CONDITIONALLY PATHOGENIC MICROORGANISMS AND FEATURES OF BIOFILM FORMATION ON THE SURFACE OF THERMOPLASTIC POLYMER MATERIAL

To cite this article:

Astashina NB, Godovalov AP, Rogozhnikova EP, Tsareva TV, Trefilova YuA, Grachev DI, Arutyunov AS. Colonization activity of conditionally pathogenic microorganisms and features of biofilm formation on the surface of thermoplastic polymer material. Stomatology. 2021;100(4):72‑76. (In Russ.)
https://doi.org/10.17116/stomat202110004172

Подписка 2025-2 (Stomatology)
 
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THE PURPOSE OF THE STUDY

Was to assess the microbial colonization and biofilm-forming activity of conditionally pathogenic microorganisms in vitro to samples of acrylic-free thermoplastic polymer material and to assess the possibility of its use for the manufacture of removable tooth splinting structures by injection method.

MATERIALS AND METHODS

The reference strains S. aureus, S. epidermidis, S. pyogenes, E. faecalis, E. coli, and C. albicans. Biofilm-forming activity was assessed by the level of ethanol extraction of a 0.1% aqueous solution of gentian violet by measuring on a PowerWave X microplate reader (USA).

RESULTS

A low degree of severity of microbial colonization and sufficient colonization resistance to the formation of a microbial biofilm of E. coli (0.374±0.056 cu opt. Pl.), S. aureus (0.272±0.039 cu. Opt. Pl.), S. epidermdis (0.299±0.028 cu opt. Pl.), S. ryogenes (0.399±0.069 cu opt. Pl.), E. faecalis (0.401±0.089 cu opt. Pl.). Moreover, C. albicans strains form a more pronounced biofilm (0.425±0.104 cu opt. Pl.) in comparison with other strains studied in the experiment.

CONCLUSION

Samples from a thermoplastic polymer based on polyoxymethylene have satisfactory biomedical characteristics and can be used at the stages of dental orthopedic treatment of patients without oral candidiasis.

Keywords:

thermoplastic materials
microbial biofilm
chronic generalized periodontitis
splint constructions

Authors:

Astashina N.B.

Perm State Medical University named after academician E.A. Wagner of the Ministry of Healthcare of the Russian Federation

Godovalov A.P.

Perm State Medical University named after academician E.A. Wagner of the Ministry of Healthcare of the Russian Federation

Rogozhnikova E.P.

Perm State Medical University named after academician E.A. Wagner of the Ministry of Healthcare of the Russian Federation

Tsareva T.V.

Moscow State University of Medicine and Dentistry

Trefilova Yu.A.

A.I. Yevdokimov Moscow State University of Medicine and Dentistry of the Ministry of Healthcare of the Russian Federation

Grachev D.I.

A.I. Yevdokimov Moscow State University of Medicine and Dentistry of the Ministry of Healthcare of the Russian Federation

Arutyunov A.S.

Central Research Institute of Dentistry and Maxillofacial Surgery

Received:

21.05.2020

Accepted:

19.04.2021

List of references:

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  2. Arutyunov SD, Tsarev VN, Babunashvili GB, Gevorkyan AA, Burdavitsina MV, Travina MV, Roshkovsky EV. Clinical aspects of microbial coloration of temporary acrylic dentures. Stomatologiya. 2008;87(1):61-64. (In Russ.).
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  5. Astashina NB, Kazakov SV, Rogozhnikova EP, Goryachev PS. Development of a non-invasive splinting structure as a therapeutic and prophylactic agent used in the treatment of patients with chronic generalized periodontitis. Problemy stomatologii. 2018;1:52-56. (In Russ.). https://doi.org/10.24411/2077-7566-2018-100010
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