PURPOSE OF THE STUDY
Evaluation of the possibility of using the method of marginal dehydration of serum for the prediction and early diagnosis of reproductive losses.
MATERIAL AND METHODS
The method of marginal dehydration was used to study the structures of the serum of 81 patients with clinical manifestations of miscarriage in the early stages. The levels of biochemical markers of pregnancy, trophoblastic beta 1-glycoprotein (TBG) and human chorionic gonadotropin (hCG), were determined using standard enzyme immunoassay (ELISA) methods. The results obtained were compared with the data of 36 healthy pregnant women.
RESULTS
As a result of the study of serum by the method of marginal dehydration in healthy women with pregnancy without signs of spontaneous abortion, parallel fissures were diagnosed much more often — markers of hyperplasia and increased proliferation (p=0.021). In various types of miscarriage, the morphological picture of a dehydrated serum drop was characterized by a violation of the length and grouping of parallel cracks, as well as the appearance of pathological structures, such as cobweb-shaped, lamellar and combined (p<0.05). The levels of TBG and hCG in the blood serum of the patients of the examined groups had not only a statistically significant difference compared to those of the control group, but also a positive correlation with pathological morphological structures (p<0.05).
CONCLUSION
As a result of the study of serum by the method of marginal dehydration, it was found that the formation of structures in the form of parallel cracks is characteristic for progressive physiological pregnancy. The formation of pathological morphotypes during non-developing pregnancy, such as lamellar and arachnoid structures, may indicate violations of the processes of cell functioning in the form of hypoxia, destruction and death. As a result of the study, a positive correlation was established between the level of TBG protein and pathological structures of a dehydrated blood serum drop. Taking into account the high degree of correlation of the obtained results, it is reasonable to assume that the method of marginal dehydration of serum can be effective for the early diagnosis of reproductive complications. Non invasiveness and ease of implementation of the method, the absence of significant economic costs allow expanding the possibilities of its application and further study in various pathological conditions, including pregnant women.
