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Pavlova T.V.

Belgorod State National Research University

Petrukhin V.A.

Moscow Regional Research Institute of Obstetrics and Gynecology

Kaplin A.N.

Kursk State Medical University

Malyutina E.S.

Belgorod State National Research University

Selivanova A.V.

2nd City Hospital

Zemlyanskaya L.O.

Clinic of Doctor Fomin LLC

New approaches in assessing the clinical and pathomorphological aspects of obstetric pathology in the structure of the mother-placenta-fetus using atomic force microscopy

Authors:

Pavlova T.V., Petrukhin V.A., Kaplin A.N., Malyutina E.S., Selivanova A.V., Zemlyanskaya L.O.

More about the authors

Journal: Russian Bulletin of Obstetrician-Gynecologist. 2021;21(1): 16‑21

DOI: 10.17116/rosakush20212101116

Read: 1020 times

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Table of contents

NEW APPROACHES IN ASSESSING THE CLINICAL AND PATHOMORPHOLOGICAL ASPECTS OF OBSTETRIC PATHOLOGY IN THE STRUCTURE OF THE MOTHER-PLACENTA-FETUS USING ATOMIC FORCE MICROSCOPY
NEW APPROACHES IN ASSESSING THE CLINICAL AND PATHOMORPHOLOGICAL ASPECTS OF OBSTETRIC PATHOLOGY IN THE STRUCTURE OF THE MOTHER-PLACENTA-FETUS USING ATOMIC FORCE MICROSCOPY

To cite this article:

Pavlova TV, Petrukhin VA, Kaplin AN, Malyutina ES, Selivanova AV, Zemlyanskaya LO. New approaches in assessing the clinical and pathomorphological aspects of obstetric pathology in the structure of the mother-placenta-fetus using atomic force microscopy. Russian Bulletin of Obstetrician-Gynecologist. 2021;21(1):16‑21. (In Russ.)
https://doi.org/10.17116/rosakush20212101116

 
Подписка 2026 Российский вестник акушера-гинеколога (Russian Bulletin of Obstetrician-Gynecologist)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

RESEARCH STUDY

The purpose is to study the structural features of the tissues (placenta, umbilical cord and uterus) and the characteristics of erythrocytes in mother-placenta-fetus system during pregnancy with thyroid gland pathology of various genesis, diabetes type 1 and gestational diabetes mellitus (GDM), moderate and severe pre-eclampsia degrees.

MATERIALS AND METHODS

The material for examination was 117 pregnant women, among whom 65 women with thyroid gland pathology, 27 women with diabetes mellitus of various genesis, 15 women with pre-eclampsia and 10 women of the control group. All women were examined with ultrasound test (Alokka-5500 machine), evaluated the levels of triiodothyronine and total thyroxine, free thyroxine, thyroid hormone and the level of thyroglobulin antibodies, glucose, including performed glucose tolerance test. In addition, we performed morphometric analysis of the tissues in the Ntegra-Aura machine and atomic force microscopy using the standard software NOVA (NT-MDT) and ImageAnalysis (NT-MDT).

RESULTS

The study of placenta and myometrium vessels revealed various circulatory problems: edge standing of red blood cells, stasis, sludge, hemolysis, endotheliocyte disconnections, and impairment of endothelial microrelief. During the study of terminal branches, we found that the villi surface and the vessel area were reduced in the sequence: GDM, euthyroid mother with thyroid pathology, hypothyroidism, diffuse toxic goiter, pre-eclampsia, and diabetes. Test revealed a significant increase deformation of red blood cells.

CONCLUSION

As a result, we noted that the pathology of thyroid gland, diabetes type 1 and pre-eclampsia deformed the structure of the mother-placenta-fetus system provoked multiple microstructural changes in tissues up with combination the anatomical and functional changes of red blood cells leading to further progression of fetal hypoxia.

Keywords:

pregnancy
preeclampsia
thyroid gland disease
diabetes mellitus type 1
gestational diabetes mellitus
tissue features of placenta and uterus
erythrocytes of the mother-placenta-fetus system
atomic force microscopy

Authors:

Pavlova T.V.

Belgorod State National Research University

Petrukhin V.A.

Moscow Regional Research Institute of Obstetrics and Gynecology

Kaplin A.N.

Kursk State Medical University

Malyutina E.S.

Belgorod State National Research University

Selivanova A.V.

2nd City Hospital

Zemlyanskaya L.O.

Clinic of Doctor Fomin LLC

Received:

17.06.2020

Accepted:

28.08.2020

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References:

  1. Dreval’ AV. Reproductive endocrinology. M.: GEOTAR-Media; 2019. (In Russ.).
  2. Burumkulova FF, Petruhin VA. Gestational diabetes mellitus: yesterday, today, tomorrow. Terapevticheskii arkhiv. 2014;86:10:109-115. (In Russ.).
  3. Kapustin RV, Onopriichuk AR, Arzhanova ON. Pathophysiology of the placenta and fetus in diabetes mellitus. Zurnal akusherstva i zhenskikh boleznei. 2018;67:6:79-92. (In Russ.). https://doi.org/10.17816/JOWD67679-92
  4. Pavlova T, Prashchayeu K, Ilnitski A, Pozdnyakova N. Erythrocytes as target cells in case of diabetes mellitus in elderly. The Journal of Nutrition, Health & Aging. 2013;17(suppl 1):776. 
  5. Filipek A, Jurewicz E. Preeclampsia — a disease of pregnant women Postepy Biochem. 2018;29:64:4:232-229.  https://doi.org/10.18388/pb.2018_146
  6. Meiramova A, Ainabekova B, Sadybekova G, Akhmetova Z, Imangazinova S, Omralina Y. Peculiarities of the course of gestation and pregnancy outcomes in women with gestational diabetes mellitus Acta Endocrinol (Buchar). 2018;14:2:213-218.  https://doi.org/10.4183/aeb.2018.213
  7. Makarov OG, Likhachov VK, Taranovska OO, Dobrovolska LM, Vashchenko VL. Role of uterine blood flow disturbances in the development of late gestosis. Wiad Lek. 2018;71:9:1719-1721.
  8. Pavlova TV, Petrukhin VA, Markovskaya VA. New in the study of the hematological features of uteroplacental blood flow in thyroid pathology during pregnancy. Gynekologiya. 2014;16:4:76-80. (In Russ.).
  9. Petrukhin VA, Logutova LS, Shidlovskaya NV, Pavlova TV, Malyutina ES, Burumkulova FF. Clinical and morphological characteristics of the fetoplacental complex in pregnant women with diffuse toxic goiter. Al’manakh klinicheskoi meditsiny. 2015;37:70-78. (In Russ.). https://doi.org/10.18786/2072-0505-2015-37-70-78
  10. Pavlova T, Petruhin V., Syrtseva I., Selivanova A., Markovskaya V., Kolesnikov D., Goncharov I, Nesterov A. Morphofunctional aspects of blood at preeclampsia. Virchows Archiv. 2015;467(suppl 1):140-143. 
  11. Pavlova T, Malutina E, Petrukhin V, Kolesnikov D, Goncharov I, Bashuk I, Markovskaya V. New morphofunctional methods of research of blood stream at diffuse toxic goiter on background of pregnancy. Virchows Archiv. 2015;467(suppl 1):143-144. 
  12. Pavlova T, Selivanova A, Syrtseva I, Petrukhin V. Innovative approaches for study of placenta at preeclampsia. Virchows Archiv. 2015;467(suppl 1):234. 
  13. David S Cooper, Peter Laurberg. Hyperthyroidism in pregnancy. Lancet Diabetes. Endocrinol. 2013;1:3:238-249.  https://doi.org/10.1016/S2213-8587(13)70086-X
  14. Bokslag A, van Weissenbruch M, Mol BW, de Groot CJ. Preeclampsia; short and long-term consequences for mother and neonate. Early Hum Dev. 2016;102:47-50.  https://doi.org/10.1016/j.earlhumdev.2016.09.007
  15. Pavlova TV, Pil’kevich NB, Zemlyanskaya LO, Zhernakov EV, Kaplin AN. Innovative approaches to diabetes mellitus study during pregnancy. Indo American Journal of Pharmaceutical Sciences. 2018;5:Issue 6:6138-6142.
  16. Pavlova TV, Petruhin VA, Syrtseva IS, Selivanova AV, Markovskaya VA. Modern approaches to the study of erythrocytes of preeclampsia in the mother-placenta-fetus. Journal of Pharmacy Research. 2017;11:Issue 12:1575-1578.
  17. Novikov YuA, Ozerin YuV, Plotnikov YuI, Rakov AV, Todua PA. Nanometrology of linear measurements in atomic force microscopy. Trudy IOFAN. 2006;62:121-143. (In Russ.).
  18. Pavlova TV, Malyutina ES, Selivanova AV, Petrukhin VA, Kaplin AN, Zemlyanskaya LO. New approaches in assessing the clinical and pathomorphological aspects of obstetric pathology in the structure of the mother-placenta-fetus using atomic force research. Systematic Reviews in Pharmacy. 2020;11:Issue 6:21-25. 
  19. Yankina SV, Shatrova NV, Berstneva SV, Pavlov DN. Pregnancy course and outcome peculiarities in women with gestational diabetes mellitus. Rossiiskii mediko-biologicheskii vestnik im. akad. I.P. Pavlova. 2018;26:1:96-105. (In Russ.). https://doi.org/10.23888/PAVLOVJ201826196-105
  20. Yarkov SP, Shilenko IV, Valiev KhKh, Karnet YuN, Snegireva NS, Yanovskii YuG. The study of the surface structure of immunochromatographic membranes by atomic force microscopy. Al’manakh klinicheskoi meditsiny. 2008;17:2:377-380. (In Russ.).
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