Objective. To evaluate the impact of intravaginal application of vitamins A, E, and C on vaginal microbiocenosis. Subjects and methods. One hundred and fifty female students of the Orenburg State Medical Academy, in whom serum vitamin A, E, and C concentrations were determined using a biofluid analyzer, the functional state of the reproductive system was evaluated according to the data of questionnaire survey, by employing physical and instrumental examinations, and vaginal microbiocenosis was analyzed by microscopic and bacteriological studies, were examined. Results. Vitamin deficiency states were shown to be common and accompanied by menstrual dysfunction and abnormal changes in lower reproductive tract microbiocenosis. The impact of intravaginal application of -tocopherol, retinol acetate, and ascorbic acid was studied in a group of young women with deficiencies of this group of vitamins on the vaginal biotope. The effect of vitamin A showed itself as an increase in vaginal contamination with both the lactoflora and opportunistic pathogens with a higher leukocyte-epithelial index. The use of vitamins E and C contributed to normalization of the vaginal microflora. Conclusion. The intravaginal application of -tocopherol to the patients with vitamin deficiency states and impaired vaginal microbiocenosis produces a more marked normalizing effect.