Studies assessing the availability of vitamins with biochemical methods after the end of a long Orthodox Lent are practically absent. Aim: to assess the sufficiency with vitamins C, B2, A, E and β-carotene by measuring their level in blood serum, as well as vitamins C, B1, B2 and B6 by measuring urine excretion of their main metabolites in people who fasted during the Great Orthodox Lent. Methods. 44 employees of the Central Clinical Hospital of St. Alexis, Metropolitan of the Moscow of the Moscow Patriarchate of the Russian Orthodox Church, were examined. They had been observing Lent from February 19 to April 7, 2018 (the average duration of fasting at the time of the examination was 40.2±0.6 days). In this group, there were 9 men and 35 women aged 43.0±1.6 years with a body mass index of 23.9±0.6 kg/m2. Vitamin status was assessed using the concentration of ascorbic acid, retinol, tocopherols, β-carotene, riboflavin in the blood serum, urinary excretion of ascorbic acid, thiamine, riboflavin and 4-pyridoxylic acid, as well as the presence of microsymptoms of micronutrient deficiency. Results. In most individuals, the concentration in the blood serum of retinol, β-carotene and α-tocopherol was within the normal range; in all the examined, the concentration of ascorbic acid corresponded to the optimal supply. The molar ratio of vitamins C and E in serum in all patients, with the exception of two women, was optimal (exceeded 1.35). A decreased level of α-tocopherol was registered in 15.8% of individuals. A deficiency of vitamins B6, B1 and B2 was found in 52.6—95.5% of the examined individuals. The fundamental difference between the vitamin status of fasting people and the vitamins sufficiency in a healthy population is the detection of a concentration of β-carotene close to the optimal level. The detection frequency and the set of clinical microsymptoms of micronutrient deficiency indicate a pronounced deficiency of vitamins of group B, D, iron, zinc in the examined people who were observing the Lent. Only one woman taking multivitamins was adequately provided with all vitamins. Conclusion. The combined reduced vitamin supply, as well as the intervitamin interactions existing in the body, indicate the advisability of taking vitamin and mineral complexes during prolonged Lent.