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Mokrysheva N.G.

Éndokrinologicheskiĭ nauchnyĭ tsentr, Moskva

Kiselev S.L.

Privolzhskiy Research Medical University, Nizhniy Novgorod, Russia

Klementieva N.V.

Privolzhskiy Research Medical University, Nizhniy Novgorod, Russia

Gorbacheva A.M.

Lomonosov Moscow State University, Moscow, Russian Federation

Dedov I.I.

Endocrinology Research Centre

The use of confocal microscopy in experimental studies and clinical practice of an endocrinologist: modern opportunities

Authors:

Mokrysheva N.G., Kiselev S.L., Klementieva N.V., Gorbacheva A.M., Dedov I.I.

More about the authors

Journal: Problems of Endocrinology. 2019;65(3): 174‑183

DOI: 10.14341/probl10140

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Table of contents

THE USE OF CONFOCAL MICROSCOPY IN EXPERIMENTAL STUDIES AND CLINICAL PRACTICE OF AN ENDOCRINOLOGIST: MODERN OPPORTUNITIES
THE USE OF CONFOCAL MICROSCOPY IN EXPERIMENTAL STUDIES AND CLINICAL PRACTICE OF AN ENDOCRINOLOGIST: MODERN OPPORTUNITIES

To cite this article:

Mokrysheva NG, Kiselev SL, Klementieva NV, Gorbacheva AM, Dedov II. The use of confocal microscopy in experimental studies and clinical practice of an endocrinologist: modern opportunities. Problems of Endocrinology. 2019;65(3):174‑183. (In Russ.)
https://doi.org/10.14341/probl10140

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Abstract:

Confocal microscopy is a modern imaging method that provides ample opportunities for in vitro and in vivo research. The clinical part of the review focuses on well-established techniques, such as corneal confocal microscopy for the diagnosis of diabetic neuropathy or endocrine ophthalmopathy; new methods are briefly described (intraoperative evaluation of tissues obtained by removing pituitary adenomas, thyroid and parathyroid glands). In the part devoted to fundamental research, the use of confocal microscopy to characterize the colocalization of proteins, as well as three-dimensional intracellular structures and signaling pathways in vivo, is considered. Indicators of intracellular calcium are analyzed.

Keywords:

confocal microscopy
endocrinology
methods

Authors:

Mokrysheva N.G.

Éndokrinologicheskiĭ nauchnyĭ tsentr, Moskva

Kiselev S.L.

Privolzhskiy Research Medical University, Nizhniy Novgorod, Russia

Klementieva N.V.

Privolzhskiy Research Medical University, Nizhniy Novgorod, Russia

Gorbacheva A.M.

Lomonosov Moscow State University, Moscow, Russian Federation

Dedov I.I.

Endocrinology Research Centre

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