Objective. To comparatively study the hemostatic properties of collagen sponge-based agents and to develop a procedure to evaluate their efficacy in stopping parenchymal hemorrhage in surgical practice. Material and methods. The hemostatic properties of new collagen sponge-based agents were investigated for efficacy in stopping parenchymal hemorrhage. In vivo experiments were carried out on 110 albino Wistar rats of both sexes weighing 180—200 g without external signs of the disease. All manipulations with the animals were used under general anesthesia (xylazine hydrochloride, zoletil); the animals were withdrawn from the experiments by overdosing the drugs for anesthesia. After midline laparotomy, the edge of the liver (wound length at a tangent to the circumference of a hepatic lobe was 1 cm; depth, 0.5 cm) and the spleen (length, 0.5 cm; depth was 0.3 cm) was alternately cut off to stop bleeding, by applying the test material with dimension of 1×1×1 cm. The findings were processed using the methods of operational statistics. The significance of differences was assessed by calculating the confidence interval. New quantitative and qualitative indicators were introduced. A technology with collagen sponges manufactured by OOO «Belkozin» and ZAO «Zelenaya Dubrava» was used as a control. Results. Contrary to expectations, monocomponent sponges (with 9% tranexamic acid or 10% cryoprecipitate) proved to be more effective; next was their combination (p<0.05). A single-agent preparation or 5% hemiblock was also more effective than a combination of hemiblock and tranexamic acid (p<0.05). Conclusion. The investigation leads to the conclusion that all the presented samples have hemostatic properties; most of them are superior to the analogues available on the market, and can be used during surgical operations on parenchymal organs, which will significantly reduce the number of postoperative complications.