Objective. To show the specific features of anterior central artery branching in relation to the variants of the circle of Willis structure, by using human male and female brain materials. Subjects and methods. The investigation was conducted using the cerebral artery preparations that had been taken from 64 men and 52 women whose age was 21 to 60 years and injected with an India ink-gelatin mixture. During the investigation, the closure and symmetry of the circle of Willis and the nature of the origin and branching of the anterolateral central arteries from their nutrient arteries were determined in terms of hemispheric lateralization and gender. Results. An assessment of the obtained results established that according to the circle of Willis type and group, the distribution of the anterolateral central arteries with a loose shape and vessel-bunch structure was similar, whereas the female and male brain preparations did not differ significantly. An analysis of the frequency of anterolateral central arteries with a vessel-bunch structure in the preparations revealed that this structure was most common in the classic versions of the circle. Conclusion. The frequency of anterolateral central arteries as a bunch of vessels depends on the circle of Willis structure.