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Bezuglova L.V.

JSC «Vector-Best»

Sergeeva E.I.

JSC «Vector-Best»

Voevodskaya L.Yu.

«Federal Center for Traumatology, Orthopedics and Endoprosthetics»

Lysykh E.E.

«CL MedicalGroup» Ltd

Polovitsa N.V.

«Amur Regional Infectious Diseases Hospital»

Deliy I.V.

JSC «Vector-Best»

Ivanov M.K.

JSC «Vector-Best»

Netesova I.G.

JSC «Vector-Best»

Netesov S.V.

Novosibirsk State University

Panel of monoclonal antibodies for determining hepatitis B virus genotypes and HBsAg subtypes: the results of testing of patient’s sera samples from three regions of Russia

Authors:

Bezuglova L.V., Sergeeva E.I., Voevodskaya L.Yu., Lysykh E.E., Polovitsa N.V., Deliy I.V., Ivanov M.K., Netesova I.G., Netesov S.V.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2023;41(2): 36‑41

DOI: 10.17116/molgen20234102136

Views: 659

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PANEL OF MONOCLONAL ANTIBODIES FOR DETERMINING HEPATITIS B VIRUS GENOTYPES AND HBSAG SUBTYPES: THE RESULTS OF TESTING OF PATIENT’S SERA SAMPLES FROM THREE REGIONS OF RUSSIA
PANEL OF MONOCLONAL ANTIBODIES FOR DETERMINING HEPATITIS B VIRUS GENOTYPES AND HBSAG SUBTYPES: THE RESULTS OF TESTING OF PATIENT’S SERA SAMPLES FROM THREE REGIONS OF RUSSIA

To cite this article:

Bezuglova LV, Sergeeva EI, Voevodskaya LYu, et al. . Panel of monoclonal antibodies for determining hepatitis B virus genotypes and HBsAg subtypes: the results of testing of patient’s sera samples from three regions of Russia. Molecular Genetics, Microbiology and Virology. 2023;41(2):36‑41. (In Russ.)
https://doi.org/10.17116/molgen20234102136

Подписка 2025-2 (Molecular Genetics, Microbiology and Virology)
 
МСФ на ЯМ
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BACKGROUND

Previously, we developed a technique using a panel of monoclonal antibodies (MAB) anti-HBs for determination of the main variants of hepatitis B virus (HBV) genotypes and surface antigen (HBsAg) subtypes circulating on the territory of Russian Federation: ayw2/D, ayw3/D, adw2/A, adrq+/C.

The Purpose was to determine HBV genotypes and HBsAg subtypes in HBsAg-positive blood serum samples from patients with chronic HBV infection from three regions of the Russian Federation using the developed reagents. We also wanted to compare the obtained data with the results of molecular biological methods for determining HBV genotypes and HBsAg subtypes and combine them with the results of previous trials.

MATERIAL AND METHODS

The comparative study included 127 HBsAg-positive blood serum samples from patients with chronic HBV infection from Barnaul, Krasnodar, Blagoveshchensk, collected in 2017-2019. We identified the HBV genotypes and HBsAg subtypes with developed reagents using enzyme-linked immunosorbent assay (ELISA) and by molecular biological methods (analysis of the DNA sequences of hepatitis B virus isolates).

RESULTS

Among 127 HBsAg positive samples, in which HBV genotypes/HBsAg subtypes were identified using the reagents we developed, the results were obtained as follows: 65 (51.6%) D/ayw2, 47 (36.5%) D/ayw3, 14 (11.1%) A/adw2, 1 (0.8%) C/adrq+. For 98 samples studied by two methods, the results of determination of HBV genotypes coincided in 100% (98/98) cases, HBsAg subtypes — in 92% (90/98) cases.

CONCLUSION

Using the developed reagents, HBV genotypes and HBsAg subtypes were determined in HBsAg-positive blood serum samples of patients with chronic HBV infection in three regions of the Russian Federation. The obtained new results of comparative tests of the developed reagents for the determination of HBV genotypes and HBsAg subtypes in HBsAg-positive blood serum samples were combined with the data previously published by us.

Keywords:

HBsAg subtype
hepatitis B virus genotype
monoclonal antibodies (MAB)
ensyme-linked immunosorbent assay (ELISA)

Authors:

Bezuglova L.V.

JSC «Vector-Best»

Sergeeva E.I.

JSC «Vector-Best»

Voevodskaya L.Yu.

«Federal Center for Traumatology, Orthopedics and Endoprosthetics»

Lysykh E.E.

«CL MedicalGroup» Ltd

Polovitsa N.V.

«Amur Regional Infectious Diseases Hospital»

Deliy I.V.

JSC «Vector-Best»

Ivanov M.K.

JSC «Vector-Best»

Netesova I.G.

JSC «Vector-Best»

Netesov S.V.

Novosibirsk State University

Received:

20.01.2023

Accepted:

28.03.2023

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