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Smirnova N.I.

FGHI Russian Research Anti-Plague Institute «Microbe»

Rybal’chenko D.A.

FGHI Russian Research Anti-Plague Institute «Microbe»

Shchelkanova E.Yu.

FGHI Russian Research Anti-Plague Institute «Microbe»

Lozovsky Yu.V.

FGHI Russian Research Anti-Plague Institute «Microbe»

Krasnov Ya.M.

FGHI Russian Research Anti-Plague Institute «Microbe»

Kutyrev V.V.

FGHI Russian Research Anti-Plague Institute «Microbe»

Variability of multiple resistance to antibiotics in cholera agent associated with different types of SXT element and spontaneous chromosome mutations

Authors:

Smirnova N.I., Rybal’chenko D.A., Shchelkanova E.Yu., Lozovsky Yu.V., Krasnov Ya.M., Kutyrev V.V.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2022;40(2): 28‑36

DOI: 10.17116/molgen20224002128

Read: 1619 times

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Table of contents

VARIABILITY OF MULTIPLE RESISTANCE TO ANTIBIOTICS IN CHOLERA AGENT ASSOCIATED WITH DIFFERENT TYPES OF SXT ELEMENT AND SPONTANEOUS CHROMOSOME MUTATIONS
VARIABILITY OF MULTIPLE RESISTANCE TO ANTIBIOTICS IN CHOLERA AGENT ASSOCIATED WITH DIFFERENT TYPES OF SXT ELEMENT AND SPONTANEOUS CHROMOSOME MUTATIONS

To cite this article:

Smirnova NI, Rybal’chenko DA, Shchelkanova EYu, Lozovsky YuV, Krasnov YaM, Kutyrev VV. Variability of multiple resistance to antibiotics in cholera agent associated with different types of SXT element and spontaneous chromosome mutations. Molecular Genetics, Microbiology and Virology. 2022;40(2):28‑36. (In Russ.)
https://doi.org/10.17116/molgen20224002128

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)
МСФ на ЯМ
Использование инфографики авторами научных работ
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Recommended articles:
Genome instability of the cholera agent: role in the evolution of pathogenicity, drug resi­stance and adaptation to a changing envi­ronment. Mole­cular Gene­tics, Microbiology and Viro­logy. 2025;(2):3-13
Abstract:

THE AIM OF THE STUDY

To analyze the variability of multiple antibiotic resistance caused by the mobile SXT element variations and chromosomal genes mutations in various strains of the cholera agent isolated in Russia and endemic cholera foci.

MATERIAL AND METHODS

Based on bioinformatic analysis of the whole genome nucleotide sequences of 110 clinical strains of V. cholerae El Tor isolated in different regions, the structure of their integrative conjugative SXT elements was determined. Mutations in the chromosomal antibiotic resistance genes gyrA, parC, and carR were identified. Phylogenetic analysis of strains containing different types of SXT was carried out.

RESULTS

It was shown that the variability of multiple antibiotic resistance in the studied strains from Russia and Ukraine was associated with the presence in the chromosome of two types of SXT elements with different compositions of resistance genes and spontaneous chromosomal mutations in the gyrA, parC, and carR genes, which determine resistance to nalidixic acid and polymyxin B. At the same time, five types of SXT were found in the studied strains from Asian and African countries, which determined a higher level of variability of their antibiotic resistance. Based on SNP analysis, an assessment of the phylogenetic relationships of isolates with different types of SXT and different chromosomal mutations is given.

CONCLUSION

The data obtained indicate that the variability of cholera agent resistance to antibiotics is provided by different types of SXT and mutations in chromosomal genes. The revealed high level of SXT genomic diversity in the V. cholerae population in cholera-endemic regions indicates the threat of importation of new cholera agent strains with previously unknown antibiotic resistance, to Russia.

Keywords:

Vibrio cholerae
SXT element
resistance to antibiotics
chromosomal mutations
SNP analysis

Authors:

Smirnova N.I.

FGHI Russian Research Anti-Plague Institute «Microbe»

Rybal’chenko D.A.

FGHI Russian Research Anti-Plague Institute «Microbe»

Shchelkanova E.Yu.

FGHI Russian Research Anti-Plague Institute «Microbe»

Lozovsky Yu.V.

FGHI Russian Research Anti-Plague Institute «Microbe»

Krasnov Ya.M.

FGHI Russian Research Anti-Plague Institute «Microbe»

Kutyrev V.V.

FGHI Russian Research Anti-Plague Institute «Microbe»

Received:

20.08.2021

Accepted:

30.09.2021

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