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Kudryavtseva T.Yu.

State Research Center for Applied Microbiology and Biotechnology of Rospotrebnadzor

Mokrievich A.N.

State Research Center for Applied Microbiology and Biotechnology of Rospotrebnadzor

Molecular genetic basis of differences in tularemia causative agent subspecies and typing of strains of Francisella tularensis subsp. holarctica

Authors:

Kudryavtseva T.Yu., Mokrievich A.N.

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Journal: Molecular Genetics, Microbiology and Virology. 2022;40(1): 12‑20

DOI: 10.17116/molgen20224001112

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MOLECULAR GENETIC BASIS OF DIFFERENCES IN TULAREMIA CAUSATIVE AGENT SUBSPECIES AND TYPING OF STRAINS OF FRANCISELLA TULARENSIS SUBSP. HOLARCTICA
MOLECULAR GENETIC BASIS OF DIFFERENCES IN TULAREMIA CAUSATIVE AGENT SUBSPECIES AND TYPING OF STRAINS OF FRANCISELLA TULARENSIS SUBSP. HOLARCTICA

To cite this article:

Kudryavtseva TYu, Mokrievich AN. Molecular genetic basis of differences in tularemia causative agent subspecies and typing of strains of Francisella tularensis subsp. holarctica. Molecular Genetics, Microbiology and Virology. 2022;40(1):12‑20. (In Russ.)
https://doi.org/10.17116/molgen20224001112

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)
МСФ на ЯМ
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Abstract:

Comparison of sequenced genomes of strains virulent for humans and animals subspecies of tularemia pathogen F. tularensis subsp. tularensis, holarctica and mediasiatica show high genomic homology — from 99.26% to 99.98%. Each year, a new and improved scheme of genome-wide phylogeny is proposed, especially for the F. tularensis subsp. holarctica, which is widespread in the world and shows low genetic diversity. Due to the limited genetic variability of the F. tularensis subsp. holarctica, sequencing as well as methods for determining the number of tandem repeats (MLVA) and detecting single nucleotide substitutions (SNPs), deletions and insertions (INDELs) in the DNA sequence that are fixed in a particular cell population remain the preferred genetic tools for molecular typing of strains within a subspecies. The current typing scheme for strains of the subspecies F. tularensis subsp. holarctica defines four main phylogenetic groups of strains (B.4, B.6, B.12 and B.16) and over 300 separate genotypic populations within this subspecies, which differ from each other by several specific single nucleotide substitutions and deletions.

Keywords:

Francisella tularensis
subspecies
typing
replacement of single nucleotides
deletions
insertions

Authors:

Kudryavtseva T.Yu.

State Research Center for Applied Microbiology and Biotechnology of Rospotrebnadzor

Mokrievich A.N.

State Research Center for Applied Microbiology and Biotechnology of Rospotrebnadzor

Received:

06.11.2020

Accepted:

19.12.2020

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