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Dergilev K.V.

Chazov National Medical Research Center of Cardiology

Guseva A.V.

Chazov National Medical Research Center of Cardiology

Tsokolaeva Z.I.

Chazov National Medical Research Center of Cardiology;
V.A. Negovsky Research Institute of General Intensive Care — Federal Scientific and Clinical Center of Intensive Care and Rehabilitation

Zubkova E.S.

Chazov National Medical Research Center of Cardiology

Goltseva Yu.D.

Chazov National Medical Research Center of Cardiology

Beloglazova I.B.

Chazov National Medical Research Center of Cardiology

Men’shikov M.Yu.

Chazov National Medical Research Center of Cardiology

Andreev A.V.

Chazov National Medical Research Center of Cardiology

Partigulov S.A.

Chazov National Medical Research Center of Cardiology

Lepilin M.G.

Chazov National Medical Research Center of Cardiology

Parfyonova Ye.V.

Chazov National Medical Research Center of Cardiology;
Lomonosov Moscow State University

Secretome of mesenchymal stromal cells of the human heart cultured in the form of spheroids is enriched with angiogenic factors and stimulates angiogenesis in vitro and in vivo

Authors:

Dergilev K.V., Guseva A.V., Tsokolaeva Z.I., Zubkova E.S., Goltseva Yu.D., Beloglazova I.B., Men’shikov M.Yu., Andreev A.V., Partigulov S.A., Lepilin M.G., Parfyonova Ye.V.

More about the authors

Journal: Russian Cardiology Bulletin. 2024;19(1): 38‑46

DOI: 10.17116/Cardiobulletin20241901138

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To cite this article:

Dergilev KV, Guseva AV, Tsokolaeva ZI, et al. . Secretome of mesenchymal stromal cells of the human heart cultured in the form of spheroids is enriched with angiogenic factors and stimulates angiogenesis in vitro and in vivo. Russian Cardiology Bulletin. 2024;19(1):38‑46. (In Russ.)
https://doi.org/10.17116/Cardiobulletin20241901138

Подписка 2025 (Russian Cardiology Bulletin)
 
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Mesenchymal stromal cells (MSCs) are a heterogeneous population of progenitor cells presenting in perivascular compartment of most tissues and performing important regulatory functions. MSCs regulate angiogenesis, neurogenesis, immune reactions and reorganization of extracellular matrix due to secretion of various biologically active molecules. This makes them important for cell therapy and tissue engineering. MSCs of bone marrow, adipose tissue and perinatal tissues are the most studied. However, there are few data on cardiac MSCs and modulation of their paracrine function to enhance regenerative properties.

OBJECTIVE

To study secretome of MSCs derived from human heart and cultured under 3D conditions (as spheroids); to evaluate its angiogenic properties. We found that human cardiac MSCs contain proangiogenic factors (VEGF, bFGF, IL8, Rantes, etc.) stimulating migration of endothelial cells in vitro and angiogenesis in vivo.

These results create a basis for further studies devoted to development of new cell-free products based on human cardiac MSC secretome for the treatment of ischemic cardiac diseases.

Keywords:

Cardiac mesenchymal cells
secretome
angiogenesis

Authors:

Dergilev K.V.

Chazov National Medical Research Center of Cardiology

Guseva A.V.

Chazov National Medical Research Center of Cardiology

Tsokolaeva Z.I.

Chazov National Medical Research Center of Cardiology;
V.A. Negovsky Research Institute of General Intensive Care — Federal Scientific and Clinical Center of Intensive Care and Rehabilitation

Zubkova E.S.

Chazov National Medical Research Center of Cardiology

Goltseva Yu.D.

Chazov National Medical Research Center of Cardiology

Beloglazova I.B.

Chazov National Medical Research Center of Cardiology

Men’shikov M.Yu.

Chazov National Medical Research Center of Cardiology

Andreev A.V.

Chazov National Medical Research Center of Cardiology

Partigulov S.A.

Chazov National Medical Research Center of Cardiology

Lepilin M.G.

Chazov National Medical Research Center of Cardiology

Parfyonova Ye.V.

Chazov National Medical Research Center of Cardiology;
Lomonosov Moscow State University

Received:

29.09.2023

Accepted:

23.11.2023

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