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Bogdanova E.O.

Pavlov First St. Petersburg State Medical University

Kochoyan Z.Sh.

Pavlov First St. Petersburg State Medical University

Anpilova A.O.

Pavlov First St. Petersburg State Medical University

Narygina D.S.

Pavlov First St. Petersburg State Medical University

Kostin N.A.

St. Petersburg State University — Research Resource Center for molecular and cell technologies

Sipovsky V.G.

Pavlov First St. Petersburg State Medical University

Dobronravov V.A.

Pavlov First St. Petersburg State Medical University

Molecular alterations of podocytes in primary focal segmental glomerulosclerosis and IgA nephropathy. (An exploratory study)

Authors:

Bogdanova E.O., Kochoyan Z.Sh., Anpilova A.O., Narygina D.S., Kostin N.A., Sipovsky V.G., Dobronravov V.A.

More about the authors

Journal: Russian Journal of Archive of Pathology. 2024;86(5): 21‑32

DOI: 10.17116/patol20248605121

Read: 1418 times

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Table of contents

MOLECULAR ALTERATIONS OF PODOCYTES IN PRIMARY FOCAL SEGMENTAL GLOMERULOSCLEROSIS AND IGA NEPHROPATHY. (AN EXPLORATORY STUDY)
MOLECULAR ALTERATIONS OF PODOCYTES IN PRIMARY FOCAL SEGMENTAL GLOMERULOSCLEROSIS AND IGA NEPHROPATHY. (AN EXPLORATORY STUDY)

To cite this article:

Bogdanova EO, Kochoyan ZSh, Anpilova AO, Narygina DS, Kostin NA, Sipovsky VG, Dobronravov VA. Molecular alterations of podocytes in primary focal segmental glomerulosclerosis and IgA nephropathy. (An exploratory study). Russian Journal of Archive of Pathology. 2024;86(5):21‑32. (In Russ.)
https://doi.org/10.17116/patol20248605121

Подписка 2026 Архив патологии (Russian Journal of Archive of Pathology)
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

OBJECTIVE

An evaluation of podocyte’s molecular phenotype alterations in primary focal segmental glomerulosclerosis (pFSGS) and IgA nephropathy (IgAN).

MATERIAL AND METHODS

The exploratory study included 14 cases of morphologically confirmed pFSGS, 14 cases of IgAN, and 12 negative controls. The negative controls comprised samples of the unaltered renal cortex obtained during laparoscopic nephrectomy in patients with malignant neoplasms of the kidney and bladder and without proteinuria. A quantitative immunomorphological study of Wilms tumour protein (WT1) expression and mesenchymal markers of podocytes (desmin and vimentin) was conducted on all kidney samples. The co-expression of the aforementioned molecules was analysed using confocal microscopy.

RESULTS

Cases of pFSGS exhibited nephrotic syndrome with proteinuria of 9.3 (3.1—14) g/24 and typical glomerular alterations in light microscopy and ultrastructural analysis. In the IgAN group, proteinuria was less severe (1.2 (0.7—1.6) g/24). The estimated glomerular filtration rate in pFSGS and IgAN was similar (pFSGS: 85 (53—103) ml/min/1.72 m², IgAN: 76 (52—87) ml/min/1.72 m²; p=0.40). In both pFSGS and IgAN, there was a reduction in WT1 expression in podocytes and an increase in vimentin expression when compared to negative controls. Compared to IgAN and controls, pFSGS exhibited a lower prevalence of glomerular WT1 expression and higher expression of desmin, which was predominantly localised in WT1-negative glomerular areas in confocal microscopy. In pFSGS, decreased nuclear expression of WT1 and increased expression of desmin were observed in the parietal epithelium of the glomerular capsule.

CONCLUSION

Bidirectional alterations in the glomerular expression of WT1 and intermediate filament proteins are apparent in pFSGS and IgAN. These findings are suggestive for the genomic reprogramming of podocytes and the parietal epithelium of the glomerulus as part of the epithelial-mesenchymal transition, determining the structural and functional disorders of these cells, more prominent in pFSGS.

Keywords:

podocytopathies
focal segmental glomerulosclerosis
IgA nephropathy
podocyte
parietal epithelium
kidney biopsy
WT1
desmin
vimentin

Authors:

Bogdanova E.O.

Pavlov First St. Petersburg State Medical University

Kochoyan Z.Sh.

Pavlov First St. Petersburg State Medical University

Anpilova A.O.

Pavlov First St. Petersburg State Medical University

Narygina D.S.

Pavlov First St. Petersburg State Medical University

Kostin N.A.

St. Petersburg State University — Research Resource Center for molecular and cell technologies

Sipovsky V.G.

Pavlov First St. Petersburg State Medical University

Dobronravov V.A.

Pavlov First St. Petersburg State Medical University

Received:

13.08.2024

Accepted:

06.09.2024

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