Possibilities of morphological diagnosis of amyloid in the subcutaneous adipose tissue biopsy specimens

Stepanova E.A.

doi:https://doi.org/10.17116/patol20228401114

Stepanova E.A.

V.M. Buyanov City Clinical Hospital of the Moscow Healthcare Department;
Russian Medical Academy of Continuious Professional Education of the Ministry of Health of Russia

SPIN RSCI: 2707-4452
ORCID: 0000-0001-7760-5858

Possibilities of morphological diagnosis of amyloid in the subcutaneous adipose tissue biopsy specimens

Authors:

Stepanova E.A.

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Journal: Russian Journal of Archive of Pathology. 2022;84(1): 14‑20

DOI: 10.17116/patol20228401114

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To cite this article:

Stepanova EA. Possibilities of morphological diagnosis of amyloid in the subcutaneous adipose tissue biopsy specimens. Russian Journal of Archive of Pathology. 2022;84(1):14‑20. (In Russ.)
https://doi.org/10.17116/patol20228401114

Подписка 2026 Архив патологии (Russian Journal of Archive of Pathology)

Использование инфографики авторами научных работ

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OBJECTIVE

To retrospectively analyze the results of histological examination of subcutaneous adipose tissue (SAT) biopsy specimens versus the diagnostic value of drugs manufactured by different procedures.

MATERIAL AND METHODS

The investigators selected and analyzed the results of biopsy studies with the simultaneous production of two different types of tissue specimens (squashed native specimens, sections from the paraffin blocks) from the abdominal SAT biopsy specimens obtained from 118 patients. In each case, the tissue specimens were stained with Congo red and examined in polarized light, concurrently using the positive control. The criterion for amyloid was the ability of congophilic deposits to birefringence with a characteristic luminescence spectrum when examined in polarized light.

RESULTS

Compared to the sections from the paraffin blocks, the native specimens were shown to be of high diagnostic value. The advantages of different types of tissue samples made from SAT biopsy specimens were formulated.

CONCLUSION

The SAT biopsy specimens are characterized by the minimal risk to a patient and by the easy way of sampling and are a frequent subject for screening biopsies. In addition to the type of amyloid, the volume, quality, and method of processing the biopsy specimen are responsible for the diagnostic value of SAT examination. The study of squashed native specimens stained with Congo red is most informative when using polarized light.

Keywords:

amyloidosis

amyloid

biopsy

subcutaneous adipose tissue

squashed specimens

native specimens

screening biopsy

Authors:

Stepanova E.A.

V.M. Buyanov City Clinical Hospital of the Moscow Healthcare Department;
Russian Medical Academy of Continuious Professional Education of the Ministry of Health of Russia

SPIN RSCI: 2707-4452
ORCID: 0000-0001-7760-5858

Received:

28.09.2021

Accepted:

10.11.2021

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References:

Benson MD, Buxbaum JN, Eisenberg DS, Merlini G, Saraiva MJM, Sekijima Y, Sipe JD, Westermark P. Amyloid nomenclature 2020: update and recommendations by the International Society of Amyloidosis (ISA) nomenclature committee. Amyloid. 2020;27(4):217-222. https://doi.org/10.1080/13506129.2020.1835263
Benson MD, Buxbaum JN, Eisenberg DS, Merlini G, Saraiva MJM, Sekijima Y, Sipe JD, Westermark P. Amyloid nomenclature 2018: recommendations by the International Society of Amyloidosis (ISA) nomenclature committee. Amyloid. 2018;25(4):215-219. https://doi.org/10.1080/13506129.2018.1549825
Lysenko LV, Rameev VV, Moiseev SV, Blagova OV, Bogdanov EI, Gendlin GE, Grishina DA, Gudkova AYa, Zakharova EV, Zinov’eva OE, Moiseeva OM, Nikitin SS, Parfenov VA, Suponeva NA, Tereshchenko SN. Clinical guidelines for diagnosis and treatment of systemic amyloidosis. Clinical Pharmacology and Therapy=Klinicheskaya farmakologiya i terapiya. 2020;29(1):13-24 (In Russ.). https://doi.org/10.32756/0869-5490-2020-1-13-24
Wisniowski B, Wechalekar A. Confirming the diagnosis of amyloidosis. Acta Haematologica. 2020;143(4):312-321. https://doi.org/10.1159/000508022
Linke RP. Congo red staining of amyloid: improvements and practical guide for a more precise diagnosis of amyloid and the different amyloidoses. In: Protein misfolding, aggregation, and conformational diseases. Springer US; 2006;239-276. https://doi.org/10.1007/0-387-25919-8_12
Sidiqi M, McPhail ED, Theis JD, Dasari S, Vrana JA, Drosou ME, Leung N, Hayman S, Rajkumar SV, Warsame R, Ansell SM, Gertz MA, Grogan M, Dispenzieri A.Two types of amyloidosis presenting in a single patient: a case series. Blood Cancer J. 2019;9(3):30. https://doi.org/10.1038/s41408-019-0193-9
Abildgaard N, Rojek AM, Møller HE, Palstrøm NB, Nyvold CG, Rasmussen LM, Hansen CT, Beck HC, Marcussen N. Immunoelectron microscopy and mass spectrometry for classification of amyloid deposits. Amyloid. 2020;7(1):59-66. https://doi.org/10.1080/13506129.2019.1688289
Ichimata S, Hata Y, Hirono K, Yamaguchi Y, Nishida N. Clinicopathological features of clinically undiagnosed sporadic transthyretin cardiac amyloidosis: a forensic autopsy-based series. Amyloid. 2021;28(2):125-133. https://doi.org/10.1080/13506129.2021.1882979
Koivuniemi R, Paimela L, Suomalainen R, Tornroth T, Leirisalo-Repo M. Amyloidosis is frequently undetected in patients with rheumatoid arthritis. Amyloid. 2008;15(4):262-268. https://doi.org/10.1080/13506120802524676
Bogov B, Lubomirova M, Kiperova B. Biopsy of subcutaneus fatty tissue for diagnosis of systemic amyloidosis. Hippokratia. 2008;12(4):236-239.
Hazenberg BP, Limburg PC, Bijzet J, van Rijswijk MH. A quantitative method for detecting deposits of amyloid A protein in aspirated fat tissue of patients with arthritis. Ann Rheum Dis. 1999;58(2):96-102. https://doi.org/10.1136/ard.58.2.96
Hazenberg BP, Bijzet J, Limburg PC, Skinner M, Hawkins PN, Butrimiene I, Livneh A, Lesnyak O, Nasonov EL, Filipowicz-Sosnowska A, Gül A, Merlini G, Wiland P, Ozdogan H, Gorevic PD, Maïz HB, Benson MD, Direskeneli H, Kaarela K, Garceau D, Hauck W, Van Rijswijk MH. Diagnostic performance of amyloid A protein quantification in fat tissue of patients with clinical AA amyloidosis. Amyloid. 2007;14(2):133-140. Erratum in: Amyloid. 2007;14(4):325. https://doi.org/10.1080/13506120701260224
Takashio S, Izumiya Y, Jinnin M, Yamamuro M, Kojima S, Ihn H, Ogawa H. Diagnostic and prognostic value of subcutaneous tissue biopsy in patients with cardiac amyloidosis. Am J Cardiol. 2012;110(10):1507-1511. https://doi.org/10.1016/j.amjcard.2012.06.059
Gameren IIV, Hazenberg BPC, Bijzet J, Rijswijk MHV. Diagnostic accuracy of subcutaneous abdominal fat tissue aspiration for detecting systemic amyloidosis and its utility in clinical practice. Arthritis Rheumatol. 2006;54(6):2015-2021. https://doi.org/10.1002/art.21902
Quarta CC, Gonzalez-Lopez E, Gilbertson JA, Botcher N, Rowczenio D, Petrie A, Rezk T, Youngstein T, Mahmood S, Sachchithanantham S, Lachmann HJ, Fontana M, Whelan CJ, Wechalekar AD, Hawkins PN, Gillmore JD. Diagnostic sensitivity of abdominal fat aspiration in cardiac amyloidosis. Eur Heart J. 2017; 38(24):1905-1908. https://doi.org/10.1093/eurheartj/ehx047