The site of the Media Sphera Publishers contains materials intended solely for healthcare professionals.
By closing this message, you confirm that you are a certified medical professional or a student of a medical educational institution.

Login
EN
+7 (495) 482-4118
+7 (495) 482-0604
+8 800 250-18-12
  • (toll-free number for subscriptions)
    Mon-Fri from 10 a.m. to 6 p.m.

  • © 1998-2025
+7 (495) 482-43-29
EN
All journals
Journal
Year
Year
Search result: 0

Ryzhova M.V.

FGBU "NII neĭrokhirurgii im. akad. N.N. Burdenko" RAMN, Moskva

Snigireva G.P.

Russian Scientific Center of Roentgen Radiology, Moscow, Russia, 117997

Golanov A.V.

FGBU "NII neĭrokhirurgii im. akad. N.N. Burdenko" RAMN, Moskva

Zheludkova O.G.

GU FNKTs detskoĭ gematologii, onkologii i immunologii Minzdrava RF im. D. Rogacheva, Moskva

Trunin Yu.Yu.

Burdenko Neurosurgical Institute, Moscow, Russian Federation

Antipina N.A.

FGBU "NII neĭrokhirurgii im. akad. N.N. Burdenko" RAMN, Moskva

Correct use of Kreatech DNA probes to detect MYC gene amplification in medulloblastomas by fluorescence in situ hybridization (in Russian only)

Authors:

Ryzhova M.V., Snigireva G.P., Golanov A.V., Zheludkova O.G., Trunin Yu.Yu., Antipina N.A.

More about the authors

Journal: Russian Journal of Archive of Pathology. 2019;81(4): 66‑72

DOI: 10.17116/patol20198104166

Read: 1468 times

Download PDF (RU)
Contact the author
Table of contents

CORRECT USE OF KREATECH DNA PROBES TO DETECT MYC GENE AMPLIFICATION IN MEDULLOBLASTOMAS BY FLUORESCENCE IN SITU HYBRIDIZATION (IN RUSSIAN ONLY)
CORRECT USE OF KREATECH DNA PROBES TO DETECT MYC GENE AMPLIFICATION IN MEDULLOBLASTOMAS BY FLUORESCENCE IN SITU HYBRIDIZATION (IN RUSSIAN ONLY)

To cite this article:

Ryzhova MV, Snigireva GP, Golanov AV, Zheludkova OG, Trunin YuYu, Antipina NA. Correct use of Kreatech DNA probes to detect MYC gene amplification in medulloblastomas by fluorescence in situ hybridization (in Russian only). Russian Journal of Archive of Pathology. 2019;81(4):66‑72. (In Russ.)
https://doi.org/10.17116/patol20198104166

Подписка 2026 Архив патологии (Russian Journal of Archive of Pathology)
МСФ на ЯМ
Использование инфографики авторами научных работ
Close metadata
Abstract:

In most cases, oncogene amplification are prognostic and predictive markers for various tumors, therefore DNA probes are unable to reveal changes in the copy numbers should not be used to diagnose malignant tumors. Objective — to comparatively analyze DNA probes from different manufacturers to detect MYC gene amplification in routine practice. Material and methods. The study material was formalin-fixed paraffin-embedded medulloblastoma fragments from 4 patients, with discrepancies in the results in the detection of MYC gene amplification. Results. MYC gene amplification was determined using DNA probes: Kreatech MYC (8q24)/SE 8, Vysis LSI MYC SO, Vysis CEP 8 (D8Z2) SG, and Zytolight SPEC MYC/CEN 8 Dual Color Probe. The use of the probes Kreatech TERC (3q26)/MYC (8q24)/SE7 Triple-Color probe failed to detect MYC gene amplification; this probe showed a balanced profile of chromosome 8. Conclusion. In routine practice, fluorescence in situ hybridization with the DNA probes Kreatech MYC (8q24)/SE 8, Vysis LSI MYC SO, Vysis CEP 8 (D8Z2) SG and Zytolight SPEC MYC/CEN 8 Dual Color Probe can be the method of choice for studying the copy number of the MYC gene. However, the authors strongly recommend that the Kreatech TERC (3q26)/MYC (8q24)/SE7 Triple-Color should not be used for this purpose. In addition, probes for fluorescence in situ hybridization must be necessarily tested in large reference laboratories dealing with one or another area of oncopathology.

Keywords:

medulloblastoma
MYC gene
amplification

Authors:

Ryzhova M.V.

FGBU "NII neĭrokhirurgii im. akad. N.N. Burdenko" RAMN, Moskva

Snigireva G.P.

Russian Scientific Center of Roentgen Radiology, Moscow, Russia, 117997

Golanov A.V.

FGBU "NII neĭrokhirurgii im. akad. N.N. Burdenko" RAMN, Moskva

Zheludkova O.G.

GU FNKTs detskoĭ gematologii, onkologii i immunologii Minzdrava RF im. D. Rogacheva, Moskva

Trunin Yu.Yu.

Burdenko Neurosurgical Institute, Moscow, Russian Federation

Antipina N.A.

FGBU "NII neĭrokhirurgii im. akad. N.N. Burdenko" RAMN, Moskva

Close metadata

References:

  1. Van Stedum S, King W. Basic FISH techniques and troubleshooting. Methods Mol Biol. 2002;204:51-63.
  2. Gu J, Smith JL, Dowling PK. Fluorescence in situ hybridization probe validation for clinical use. Methods Mol Biol. 2017;1541:101-118.
  3. Tansatit M. Applications of fluorescence in situ hybridization technology in malignancies. Methods Mol Biol. 2017;1541:75-90.
  4. Tibiletti MG. Interphase FISH as a new tool in tumor pathology. Cytogenet Genome Res. 2007;118(2-4):229-236. https://doi.org/10.1159/000108305
  5. Tibiletti MG. Specificity of interphase fluorescence in situ hybridization for detection of chromosome aberrations in tumor pathology. Cancer Genet Cytogenet. 2004;155:143-148. https://doi.org/10.1016/j.cancergencyto.2004.03.005
  6. Aldosari N, Bigner SH, Burger PC, Becker L, Kepner JL, Friedman HS, McLendon RE. MYCC and MYCN oncogene amplification in medulloblastoma. A fluorescence in situ hybridization study on paraffin sections from the Children’s Oncology Group. Arch Pathol Lab Med. 2002;126(5):540-544.
  7. Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, eds. WHO Classification of tumours of the central nervous system. 4th ed. Lyon: IARC, 2016.
  8. Korshunov A, Chavez L, Northcott PA, Sharma T, Ryzhova M, Jones DTW, von Deimling A, Pfister SM, Kool M. DNA-methylation profiling discloses significant advantages over NanoString method for molecular classification of medulloblastoma. Acta Neuropathol. 2017;134(6):965-967. https://doi.org/10.1007/s00401-017-1776-9
  9. Korshunov A, Sahm F, Zheludkova O, Golanov A, Stichel D, Schrimpf D, Ryzhova M, Potapov A, Habel A, Meyer J, Lichter P, Jones DTW, von Deimling A, Pfister SM, Kool M. DNA-methylation profiling is a method of choice for molecular verification of pediatric WNT activated medulloblastomas. Neuro Oncol. 2018; Sep25. https://doi.org/10.1093/neuonc/noy155
  10. Koelsche C, Kriegsmann M, Kommoss FKF, Stichel D, Kriegsmann K, Vokuhl C, Grünewald TGP, Romero-Pérez L, Kirchner T, de Alava E, et al. DNA methylation profiling distinguishes Ewing-like sarcoma with EWSR1-NFATc2 fusion from Ewing sarcoma. J Cancer Res Clin Oncol. 2019;145(5):1273-1281. https://doi.org/10.1007/s00432-019-02895-2
Contact the author
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.

Email Confirmation

An email was sent to test@gmail.com with a confirmation link. Follow the link from the letter to complete the registration on the site.

Email Confirmation

Contact us
If you have any questions, complaints or suggestions, please use this feedback form.
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.
Submit Application

You can become an author of one of our magazines, send a request and we will consider it in during the day.

Name
Phone
E-mail
Message
Login
Registration
E-mail
Password
Forgot Password?

Log in to the site using your account in one of the services

Password recovery
E-mail
Login
Register

We use cооkies to improve the performance of the site. By staying on our site, you agree to the terms of use of cооkies. To view our Privacy and Cookie Policy, please. click here.