The article is devoted to structural changes in the regional lymph nodes of the middle ear (superficial, facial and deep cervical lymph nodes) in experimental animals (30 male Wistar rats) when modeling exudative otitis media and after a 7-day course of local ultrasound lymphotropic therapy. The method of conducting the experiment is described. Comparative morphological and morphometric studies of lymph nodes were carried out on the 12th day from the beginning of otitis modeling and evaluated according to 19 criteria: node cut-off area, capsule area, marginal sinus, interstitial part, paracortical area, cerebral sinuses, brain cords, area number and area of primary and secondary lymphoid nodules, area of germinal centers, specific areas of the cortex and medulla oblongata, sinus system, T-dependent and B-dependent zones, cortical-cerebral index. With exudative otitis media in the regional lymph nodes of the middle ear, compared with the physiological norm, a reaction from the intra-nodular structures was observed, indicating inhibition of drainage and detoxification in the lymph catchment area, which was considered as the morphological equivalent of the insufficiency of their lymphocyte function. Regional lymphotropic therapy using low-frequency ultrasound contributed to the positive dynamics of the structural components of the lymph nodes and the normalization of most of their indicators, which is a prerequisite for the development and use of this technology in clinical practice.