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Svistushkin V.M.

Sechenov First Moscow State Medical University

Timashev P.S.

Institute for Regenerative Medicine of the I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)

Shekhter A.B.

Institute for Regenerative Medicine of the I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)

Zolotova A.V.

I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)

Mokoyan Zh.T.

Sechenov First Moscow State Medical University

Svistushkin M.V.

I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)

Experimental evidence for regenerative treatment of chronic tympanic membrane perforation

Authors:

Svistushkin V.M., Timashev P.S., Shekhter A.B., Zolotova A.V., Mokoyan Zh.T., Svistushkin M.V.

More about the authors

Journal: Russian Bulletin of Otorhinolaryngology. 2020;85(6): 23‑26

DOI: 10.17116/otorino20208506123

Views: 940

Downloaded: 49

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Table of contents

To cite this article:

Svistushkin VM, Timashev PS, Shekhter AB, Zolotova AV, Mokoyan ZhT, Svistushkin MV. Experimental evidence for regenerative treatment of chronic tympanic membrane perforation. Russian Bulletin of Otorhinolaryngology. 2020;85(6):23‑26. (In Russ.)
https://doi.org/10.17116/otorino20208506123

Подписка 2025 (Russian Bulletin of Otorhinolaryngology)
 
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OBJECTIVE

The aim of our study was an experimental evaluation of tissue engineering approach to chronic tympanic membrane perforation closure.

MATERIALS AND METHODS

Chronic tympanic membrane perforation models were created both sides in 12 chinchillas. Right sided perforations were divided into two equal groups (A and B) according to treatment; left sided perforations were used as a control group. Group A perforations were treated with collagen scaffold and fibroblast growth factor, group B perforations were treated with collagen scaffold only. During follow-up, we provided otovideoendoscopy for closure rates assessement every 2 weeks. In case of perforation closure, a morphological investigation of the regenerate was performed.

RESULTS

Group A perforations were totally closed 2 weeks after treatment in all animals. In group B, complete closure of perforation was achieved after the third treatment procedure in one case. There were no spontaneous perforation closure in the control group. According to morphological investigation, the restoration of trilaminar structure was observed only in tissue engineering group tympanic membranes.

Keywords:

chronic tympanic membrane perforation
tissue engineering
fibroblast growth factor
scaffold

Authors:

Svistushkin V.M.

Sechenov First Moscow State Medical University

Timashev P.S.

Institute for Regenerative Medicine of the I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)

Shekhter A.B.

Institute for Regenerative Medicine of the I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)

Zolotova A.V.

I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)

Mokoyan Zh.T.

Sechenov First Moscow State Medical University

Svistushkin M.V.

I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)

Received:

12.03.2020

Accepted:

25.04.2020

List of references:

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  2. Langer R, Vacanti J. Advances in tissue engineering. J Pediatr Surg. 2016;51(1):8-12.  https://doi.org/10.1016/j.jpedsurg.2015.10.022
  3. Ghezzi C, Rnjak-Kovacina J, Kaplan D. Corneal Tissue Engineering: Recent Advances and Future Perspectives. Tissue Engineering Part B: Reviews. 2015;21(3):278-287.  https://doi.org/10.1089/ten.teb.2014.0397
  4. Villar-Fernandez M, Lopez-Escamez J. Outlook for tissue engineering of the tympanic membrane. Audiol Res. 2015;5(1). https://doi.org/10.4081/audiores.2015.117
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  6. Hong P, Bance M, Gratzer P. Repair of tympanic membrane perforation using novel adjuvant therapies: A contemporary review of experimental and tissue engineering studies. Int J Pediatr Otorhinolaryngol. 2013;77(1):3-12.  https://doi.org/10.1016/j.ijporl.2012.09.022
  7. Teh B, Marano R, Shen Y, Friedland P, Dilley R, Atlas M. Tissue Engineering of the Tympanic Membrane. Tissue Engineering Part B: Reviews. 2013;19(2):116-132.  https://doi.org/10.1089/ten.teb.2012.0389
  8. Kanemaru S, Umeda H, Kitani Y, Nakamura T, Hirano S, Ito J. Regenerative Treatment for Tympanic Membrane Perforation. Otology & Neurotology. 2011;32(8):1218-1223.
  9. Kato M, Jackler RK. Repair of chronic tympanic membrane perforations with fibroblast growth factor. Otolaryngol Head Neck Surg. 1996;115(6):538-547. 
  10. Chattopadhyay S, Raines RT. Review collagen-based biomaterials for wound healing. Biopolymers. 2014;101(8):821-833.  https://doi.org/10.1002/bip.22486
  11. Glowacki J, Mizuno S. Collagen scaffolds for tissue engineering. Biopolymers. 2008;89(5):338-344.  https://doi.org/10.1002/bip.20871

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