The objective of the present study was the comparative analysis of acoustic parameters of the voice in the subjects of different age groups. The phonetographic analysis involved 85 relatively healthy subjects (40 men and 45 women) divided into two age-matched groups. Group 1 included mature adults at the age of 43-59 years (n=43), group 2 was comprised of the aged and elderly persons (60-85 years, n=42). It was shown that the frequency of the primary tone decreases with age not only in women but also in men even though the decrease is less pronounced in the latter group. The process of ageing is accompanied by the narrowing of the frequency and dynamic range of the voice, the increase of Jitter, and the decrease in the strength of the voice. As a result, the dyshonia severity index (DSI) shifts toward negative values. These changes are more pronounced in women compared with men. Age-specific negative dynamics of maximum phonation time was not documented.