Aim of the study: to estimate the possibility of application of the autodyne method for the detection of nano-displacements of the tympanic membrane and diagnostics of ear diseases. Methods: nano-displacements of the tympanic membrane were revealed with the use of the original laser-assisted autodyne technique for the measurement of nano-vibrations. The majority of the measurements were performed in the free auditory field. A total of 182 patients allocated to 7 groups depending on the form of hearing disturbances or their absence were involved in the study. Results: the values of nano-displacement of the tympanic membrane at different levels of acoustic pressure have been obtained for otologically healthy individuals. The age dependence of this parameter was investigated in the absence of otological pathology. Similar studies were carried out in different groups of patients with the diseases of the middle and internal ears. The mobility of the tympanic membrane was shown to be significantly altered in the presence of adhesive events in the middle ear and pathological changes in the Eustachian tube. The possibilities of the clinical application of the proposed method have been estimated.