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Ignatova E.N.

National Research Center for Hematology, Ministry of Health of Russia, Moscow, Russia

Tupoleva T.A.

National Research Center for Hematology, Ministry of Health of Russia, Moscow, Russia

Ovchinnikova E.N.

National Research Center for Hematology, Ministry of Health of Russia, Moscow, Russia

Romanova T.Yu.

National Research Center for Hematology, Ministry of Health of Russia, Moscow, Russia

Yaroslavtseva N.G.

National Research Center for Hematology, Ministry of Health of Russia, Moscow, Russia

Filatov F.P.

I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

Troitskaya V.V.

Federal state budgetary institution 'Hematological Research Centre', Russian Ministry of Health, Moscow, Russia

Kuz'mina L.A.

Gematologicheskiĭ nauchnyĭ tsentr Minzdrava Rossii, Moskva

Parovichnikova E.N.

Federal state budgetary institution 'Hematological Research Centre', Russian Ministry of Health, Moscow, Russia

Gaponova T.V.

Hematology Research Center, Ministry of Health of Russia, Moscow, Russia

Savchenko V.G.

National Research Center for Hematology, Moscow, Russia

Impact of current approaches to laboratory screening of donated blood and its components on hepatitis B virus infection in patients with blood system diseases

Authors:

Ignatova E.N., Tupoleva T.A., Ovchinnikova E.N., Romanova T.Yu., Yaroslavtseva N.G., Filatov F.P., Troitskaya V.V., Kuz'mina L.A., Parovichnikova E.N., Gaponova T.V., Savchenko V.G.

More about the authors

Journal: Therapeutic Archive. 2017;89(11): 27‑34

DOI: 10.17116/terarkh2017891127-34

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Table of contents

IMPACT OF CURRENT APPROACHES TO LABORATORY SCREENING OF DONATED BLOOD AND ITS COMPONENTS ON HEPATITIS B VIRUS INFECTION IN PATIENTS WITH BLOOD SYSTEM DISEASES
IMPACT OF CURRENT APPROACHES TO LABORATORY SCREENING OF DONATED BLOOD AND ITS COMPONENTS ON HEPATITIS B VIRUS INFECTION IN PATIENTS WITH BLOOD SYSTEM DISEASES

To cite this article:

Ignatova EN, Tupoleva TA, Ovchinnikova EN, et al. . Impact of current approaches to laboratory screening of donated blood and its components on hepatitis B virus infection in patients with blood system diseases. Therapeutic Archive. 2017;89(11):27‑34. (In Russ.)
https://doi.org/10.17116/terarkh2017891127-34

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Abstract:

Aim. To evaluate the detection rate of markers for hepatitis B virus (HBV) in the blood samples taken from patients with blood system diseases, by applying the current approaches to examining donated blood and its components for markers of viral infections. Materials and methods. The investigation included blood samples from patients with blood system diseases (n=364) and donors (n=5,011). The results of laboratory screening of donated blood samples (n=13,081) were retrospectively analyzed. Commercial kits of reagents were used for immunochemical assay and polymerase chain reaction. Results. Patients with blood system diseases were recorded to have markers of active HBV infection in 12.6% of cases, anti-HBc in 31.3%, and anti-HBs in 37.6%. A retrospective analysis of the results of screening donated blood samples showed the presence of markers for active HBV infection in 0.28% of cases. A prospective examination of blood donors revealed markers of HBV infection in 4.83% of cases, including those of active forms in 0.54% and anti-HBc in 4.79%. The markers of active HBV infection in donors were only anti-HBc IgM in 0.42% of cases. The blood samples from donors with an anti-HBs titer of >200 mIU/ml contained anti-HBc IgM in 10.5%. Conclusion. In the last 5-7 years, the detection rate of markers of HBV infection in the blood samples of patients with blood system diseases have remained at a high level. Screening for decreed markers fails to identify people with inapparent infections among the donors. Even high anti-HBs concentrations in the donated blood may be a risk for HBV transmission by transfusion to a recipient.

Keywords:

hepatitis B virus
hepatitis B virus core antigen antibodies
patients with blood system diseases
transfusion mode of transmission

Authors:

Ignatova E.N.

National Research Center for Hematology, Ministry of Health of Russia, Moscow, Russia

Tupoleva T.A.

National Research Center for Hematology, Ministry of Health of Russia, Moscow, Russia

Ovchinnikova E.N.

National Research Center for Hematology, Ministry of Health of Russia, Moscow, Russia

Romanova T.Yu.

National Research Center for Hematology, Ministry of Health of Russia, Moscow, Russia

Yaroslavtseva N.G.

National Research Center for Hematology, Ministry of Health of Russia, Moscow, Russia

Filatov F.P.

I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

Troitskaya V.V.

Federal state budgetary institution 'Hematological Research Centre', Russian Ministry of Health, Moscow, Russia

Kuz'mina L.A.

Gematologicheskiĭ nauchnyĭ tsentr Minzdrava Rossii, Moskva

Parovichnikova E.N.

Federal state budgetary institution 'Hematological Research Centre', Russian Ministry of Health, Moscow, Russia

Gaponova T.V.

Hematology Research Center, Ministry of Health of Russia, Moscow, Russia

Savchenko V.G.

National Research Center for Hematology, Moscow, Russia

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References:

  1. Garmaeva TTs, Kulikov SM, Mikhailova EA, Igla RE, Shmatova TF, Gemdzhyan EG, i dr. Dinamika infitsirovaniya virusami gepatitov V i S bol’nykh s zabolevaniyami sistemy krovi. Gematologiya i transfuziologiya. 2009;54(5):16-23. (In Russ.)
  2. Garmaeva TTs. Virusnye gepatity V i S u bol’nykh zabolevaniyami sistemy krovi. Diss. na soiskanie uchenoi stepeni doktora med nauk Moskva 2012 Ssylka aktivna na 30.05.2016. Dostupno po: http://www.dissercat.com (In Russ.)
  3. Hatu G, Brumboiu MI, Gorgan IN, Bocşan IS. Romanian blood donors screening: is it really necessary and/or mandatory? Rev Med ChirSoc Med Nat Iasi. 2013;117(3):741-746. Accessed May 30, 2016. Available at: http://www.ncbi.nlm.nih.gov/pubmed/24502043
  4. Garmaeva TTs, Kulikov SM, Sudarikov AB, Filatov FP, Mikhailova EA, Gorodetskii VM, Savchenko VG. O neobkhodimosti ispol’zovaniya metodov genodiagnostiki dlya testirovaniya donorov krovi, komponentov krovi i retsipientov mnogochislennykh gemotransfuzii. Gematologiya I transfuziologiya. 2011;4:36-39. (In Russ.)
  5. Margaritis AR, Brown SM, Seed CR, Kiely P, et al. Comparison of two automated nucleic acid testing systems for simultaneous detection of human immunodeficiency virus and hepatitis C virus RNA and hepatitis B virus DNA. Transfusion. 2007;47(10):1783-1793. https://doi.org/10.1111/j.1537-2995.2007.01343.x
  6. Reesink HW, Engelfriet CP, Henn G, et al. Occult hepatitis B infection in blood donors. Vox Sang. 2008;94(2):153-166. https://doi.org/10.1111/j.1423-0410.2008.01017.x
  7. Slotz M, Tinguely C, Graziani M, et al. Efficacy of individual nucleic acid amplification testing in reducing the risk of transfusion-transmitted hepatitis B virus infection in Switzerland, a low endemic region. Transfusion. 2010;50(12):2695-2706. https://doi.org/10.1111/j.1537-2995.2010.02732.x
  8. Belyakova VV. Sovershenstvovanie laboratornogo testirovaniya dlya obespecheniya virusnoi bezopasnosti allogennykh gemokomponentov. Dissertatsii na soiskanie uchenoi stepeni kand. biol. nauk. Moskva. 2014g Ssylka aktivna na 30.05.2016. http://www.dissercat.com (In Russ.)
  9. Bogoslovskaya EV, Tsygankova GM, Shipulin GA. Skrining donorskoi krovi s po-moshch’yu mole-kulyarno-geneticheskikh metodov FGUN «Tsentral’nyi NII epidemiologii» Rospotrebnadzora. Moskva, Laboratornaya meditsina. 2008;9. Ssylka aktivna na 30.05.2016. http://md2007.org/ru/programm.pdf (In Russ.)
  10. Filatov FP. Virusnaya bezopasnost’ perelivaniya krovi. Priroda. 2005;3. Ssylka aktivna na 30.05.2016. http://vivovoco.astronet.ru/VV/JOURNAL/NATURE/03_05/VIRUS.HTM (In Russ.)
  11. Zhiburt EB, Shestakov EA, Kodenev AT i dr. Novoe v transfuziologii (na Kh1Kh regional’nom kongresse mezhdunarodnogo obshchestva perelivaniya krovi). Transfuziologiya. 2009;3-4:64-91. (In Russ.)
  12. Stramer SL, Wend U, Candotti D, Foster G.A, Hollinger FB, Dodd RY, et al. Nucleic acid testing to detect HBV infection in blood donors. N Engl J Med. 2011;364:236-247. https://doi.org/10.1056/NEJMoa1007644
  13. Kupek E. Residual risk of hepatitis-B-infected blood donations: estimation methods and perspectives. Review article. ISRN Infectious Diseases. 2013:15. https://doi.org/10.5402/2013/839896
  14. Gubanova MN, Mad-zaev SR, Avetisyan KS, Bakhmet’ev AV, Burkitbaev ZhK, Zarubin MV,Karavaev AV, Klyueva EA, Kodenev AV, Kuz-netsov KV, Maksimov AR, SkorikovaSV, Sultanbaev US, Filina NG, Khal’zov KV, Yakovleva LM, Zhiburt EB. Raschetnyi osta-tochnyi risk infitsirovaniya pri transfuzii. Infektsii v transfuziologii i transplantologii. Molekulyarnaya diagnostika. 2014;1. Ssylka aktivna na 30.05.2016. http://cyberleninka.ru/article/n/raschetnyy-ostatochnyy-risk-infitsirovaniya-pri-perelivanii-krovi (In Russ.)
  15. Prikaz Minzdrava RF ot 14.09.2001 №364 (red. ot 06.06.2008) «Ob utverzhdenii Poryadka meditsinskogo obsledovaniya donora krovi i ee komponentov» (Zaregistrirovano v Minyuste RF 31.10.2001 №3009) Ssylka aktivna na 30.05.2016. http://www.consultant.ru/document/cons_doc_LAW_78073/© Konsul’tantPlyus, 1992-2015 (In Russ.)
  16. Golosova TV, Bondarenko IA. Transfuzionnye infektsii: epidemiologiya, diagnostika v sluzhbe krovi. Vestnik sluzhby krovi Rossii. 2007;1:16-21. (In Russ.)
  17. Zakharov VV, Kolyshkin VM, Oprish-chenko SA, Rusanov VM. Virusnaya bezopasnost’ donorskoi plazmy i lechebnykh preparatov krovi, Epidemiologiya i vaktsinoprofilaktika. 2011;4(59). (In Russ.)
  18. Tarasenko OA, Gukasyan IA, Sobolevskaya LV, Chernenko TV. Opyt ispol’zovaniya metoda PTsR dlya vyyavleniya RNK(DNK) vozbuditelei virusnykh gepatitov V i S u seronegativnykh donorov. Transfuziologiya. 2010;2. Ssylka aktivna na 30.05.2016. http://transfusion-web.ru (In Russ.)
  19. Yaroslavtseva NG, Grumbkova LO, Tupoleva TA, Ignatova EN, Somova AV, Gulyaeva AA i dr. Virusnaya bezopasnost’ gemotransfuzii: obespechivayut li ee prinyatye laboratornye metody vybrakovki donorskoi krovi po gepatitam V i S. Gematologiya i transfuziologiya. 2006;2:22-26. (In Russ.)
  20. Raimondo G, Allain JP, Brunetto MR, Buendia MA, Chen DS, ColomboM, Craxi A, Donato F, Ferrari C, Gaeta GB, GerlichWH, LevreroM, Locarnini S, Michalak T, Mondelli MU, Pawlotsky JM, Pollicino T, Prati D, Puoti M, Samuel D, Shouval D, Smedile A, Squadrito G, Trepo C, Villa E,Will H, Zanetti AR, ZoulimF. Statements from the Taormina expert meeting on occult hepatitis B virus infection. Journal of Hepatology. 2008;49:652-657. https://doi.org/10.1016/j.jhep.2008.07.014
  21. Ammosov AD. Gepatit V. Informatsionno-metodicheskoe posobie. Novosibirsk: «Vektor-Best»; 2006. (In Russ.)
  22. Ros K, Veber M, Petersen D, Drosten K, Bur S, Sirais V, i dr. HBV NAT i ANTI-HBc- HBV NAT i ANTI-HBc-testirovanie povyshajut bezopasnost’ krovi. Institut transfuzionnoi meditsiny i immunogematologii Germanskogo Krasnogo Kresta, referat, Rossiisko-nemetskii nauchno-metodicheskii sbornik, Moskva — Novosibirsk — Frankfurt-na-Maine, 2004:38-55. Ssylka aktivna na 30.05.2016. http://www.zavlab.ru/files/pdf/garantiya.pdf (In Russ.)
  23. Vozmozhnost', effektivnost' i sebestoimost' NAT-testirovaniya v Germanii. Vestnik Sluzhby krovi Rossii. 2004;2:47-49. http://www.fskl.ru/wp-content/uploads/2013/07/2004.pdf
  24. Roth WK. Quarantine Plasma: Quo vadis? Transfus Med Hemother. 2010;37:118-122. https://doi.org/10.1159/000314710
  25. WHO: Screening donated blood for transfusion-transmissible infections: recommendations. Geneva; 2009. Accessed May 30, 2016. Available from http://apps.who.int/iris
  26. Katz L, Strong DM, Tegtmeier G, Stramer S. Performance of an algorithm for the reentry of volunteer blood donors deferred due to false-positive test results for antibody to hepatitis B core antigen. Transfusion. 2008;48(11):2315-2322. https://doi.org/10.1111/j.1537-2995.2008.01844.x
  27. Department of Health Advisory Group on- Hepatitis: Protecting health care workers and patients from hepatitis B. Recommendations: of the Advisory Group on Hepatitis. HMSO, 1993, London. Accessed May 30, 2016. Available at: http://webarchive.nationalarchives.gov.uk/+/www.dh.gov.uk/en/Publicationsandstatistics/Lettersandcirculars/Healthserviceguidelines/DH_4084234
  28. Sato S, Ohhashi W, Ihara H, et al. Comparison of the sensitivity of NAT using pooled donor samples for HBV and that of a serologic HBsAg assay. Transfusion. 2001;41(9):1107-1113. https://doi.org/10.1046/j.1537-2995.2001.41091107.x
  29. Satake M, Taira R, Yugi H, et al. Infectivity of blood components with low hepatitis B virus DNA levels identified in a lookback program. Transfusion. 2007;47(7):1197-1205. https://doi.org/10.1111/j.1537-2995.2007.01276.x
  30. Somova AV, Tupoleva TA, Yaroslavtseva NG, Grumbkova LO, Romanova TYu, Golosova TV, Filatov FP. Sravnitel’noe izuchenie chastoty vyyavleniya markerov virusa gepatita V (VGV) v IFA i PTsR u gematologicheskikh bol’nykh. Rossiiskii zhurnal gastroenterologii, gepatologii, koloproktologii. Prilozhenie №31. Materialy 13 Rossiiskoi konferentsii «Gepatity segodnya» Moskva, 17-19 marta 2008:40-30. (In Russ.)
  31. Gerlich WH. Medical virology of hepatitis B: how it began and where we are now. Virol J. 2013;10:239. https://doi.org/10.1186/1743-422X-10-239
  32. Garmaeva TTs, Kulikov SM, Karyakin AV, Terent’eva LA, Tupoleva TA, Grumbkova LO, Yaroslavtseva NG, Somova AV, Filatov FP, Fevraleva IS, Makarik TV, Glinshchikova OA, Sudarikov AB, Mikhailova EA, Savchenko VG. Monitorirovanie faktorov riska i indikatorov infitsirovannosti virusami gepatitov V i S u gematologicheskikh bol’nykh. Gematologiya i transfuziologiya. 2006;1:23-27. (In Russ.)
  33. Huber-Wagner S, Qvick M, Mussack T, Euler E, Kay MV, Mutschler W, Kanz K-G and Working Group on Polytrauma of the German Trauma Society (DGU). Massive blood transfusion and outcome in 1062 polytrauma patients: a prospective study based on the Trauma Registry of the German Trauma Society. VoxSanguinis. 2007;92(1):69-78. https://doi.org/10.1111/j.1423-0410.2006.00858.x
  34. Vermeulen M, Dickens C, Lelie N, Walker E, Coleman C, Keyter M, Reddy R, Crookes R, Kramvis A. Hepatitis B virus transmission by blood transfusion during 4 years of individual-donation nucleic acid testing in South Africa: estimated and observed window period risk. Transfusion. 2012;52(4):880-892. https://doi.org/10.1111/j.1537-2995.2011.03355.x
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