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Kupriyanov D.D.

Russian Center of Forensic Medical Expertise

Fedulova M.V.

Russian Center of Forensic Medical Expertise

Dermal mast cells density examination as an aspect of mechanical injuries vitality diagnostics

Authors:

Kupriyanov D.D., Fedulova M.V.

More about the authors

Journal: Forensic Medical Expertise. 2024;67(6): 25‑29

DOI: 10.17116/sudmed20246706125

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Table of contents

DERMAL MAST CELLS DENSITY EXAMINATION AS AN ASPECT OF MECHANICAL INJURIES VITALITY DIAGNOSTICS
DERMAL MAST CELLS DENSITY EXAMINATION AS AN ASPECT OF MECHANICAL INJURIES VITALITY DIAGNOSTICS

To cite this article:

Kupriyanov DD, Fedulova MV. Dermal mast cells density examination as an aspect of mechanical injuries vitality diagnostics. Forensic Medical Expertise. 2024;67(6):25‑29. (In Russ.)
https://doi.org/10.17116/sudmed20246706125

Подписка 2025-2 (Forensic Medical Expertise)
 
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OBJECTIVE

To examine the changes in dermal mast cells density in the mechanically injured skin and to evaluate the applicability of dermal mast cells density increase as an injuries vitality diagnostic criteria (serial examination, statistically processed results).

MATERIAL AND METHODS

240 skin autopsy samples with mechanical injuries from 40 persons were divided to 3 groups (80 in each group): vital injuries, postmortal injuries, control non-injured samples. A routine histological examination using standard H&E stain and IHC with mast cells tryptase antibodies was performed consequented with histological slides full-format scanning and computer processing.

RESULTS AND CONCLUSION

An increase in the density of dermal mast cells relative to control samples was found in 80% of samples with vital injuries and in 50% with postmortem ones. Statistically significant differences in mast cell density were revealed between samples of all 3 groups, but they cannot be used as a reliable vitality diagnostic method.

Keywords:

injuries vitality
mast cells
tryptase
IHC
forensic pathology

Authors:

Kupriyanov D.D.

Russian Center of Forensic Medical Expertise

Fedulova M.V.

Russian Center of Forensic Medical Expertise

Received:

03.07.2024

Accepted:

08.07.2024

List of references:

  1. Fedulova MV, Kupriyanov DD. Reliability of the immunohistochemical estimation of vitality and injury age: analysis and perspectives for study. Forensic Medical Expertise. 2020;63(2):52-57. (In Russ.). https://doi.org/10.17116/sudmed20206302152
  2. Fedulova MV, Kupriyanov DD. TGFβ1 antibodies application for IHC determination of injuries vitality. The experimental results. Forensic Medical Expertise. 2023;66(5):43-46. (In Russ.). https://doi.org/10.17116/sudmed20236605143
  3. Kupriyanov DD, Fedulova MV. Detection of some markers of the early phase of the inflammatory and reparative process in mechanical skin injuries. Forensic Medical Expertise. 2024;67(2):17-19. (In Russ.). https://doi.org/10.17116/sudmed20246702117
  4. Fedulova MV, Kupriyanov DD. Primenenie antitel k TGFß1 dlya immunogistohimicheskogo ustanovleniya prizhiznennosti povrezhdenij kozhi tupy`mi tverdy`mi predmetami: metodicheskie rekomendacii. Moscow: Izdatel`stvo OOO «Print»; 2024. (In Russ.).
  5. Fedulova MV, Kupriyanov DD. Degranulyaciya dermal`ny`h tuchny`h kletok v aspekte morfologicheskoj diagnostiki prizhiznennosti povrezhdenij. Sudebnaya medicina: voprosy`, problemy`, ekspertnaya praktika. Materialy` nauchno-prakticheskoj konferencii, posvyashhennoj 30-letiyu Mezhregional`noj obshhestvennoj organizacii Sudebny`e mediki Sibiri. Tomsk; 2023. (In Russ.).
  6. Bankhead P, Loughrey MB, Fernández JA, et al. QuPath: Open source software for digital pathology image analysis. Scientific Reports. 2017;7(1):16878. https://doi.org/10.1038/s41598-017-17204-5
  7. Bonelli A, Bacci S, Vannelli GB, Norelli GA. Immunohistochemical localization of mast cells as a tool for the discrimination of vital and postmortem lesions. Int J Legal Med. 2003;117:14-18.  https://doi.org/10.1007/s00414-002-0304-0
  8. Janssens AS, Heide R, den Hollander JC, et al. Mast cell distribution in normal adult skin. J Clin Pathol. 2005;58(3):285-289.  https://doi.org/10.1136/jcp.2004.017210
  9. Petrov VV, Vasilyeva OV, Kornilova NK, Gunin AG. Age Related Changes in Mast Cells and Eosinophils of Human Dermis. Ontogenez, 2013;44(3):179-185. (In Russ.). https://doi.org/10.7868/S0475145013030051

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