New polymorphic DNA marker to determine a person’s sex from biological material

Garafutdinov R.R.

Institute of Biochemistry and Genetics Ufa Federal Research Centre of the Russian Academy of Sciences

Sakhabutdinova A.R.

Institute of Biochemistry and Genetics Ufa Federal Research Centre of the Russian Academy of Sciences

Aminev F.G.

Institute of law, Bashkir State University

Chemeris A.V.

Kazakhstan Medical University of Continuing Education

New polymorphic DNA marker to determine a person’s sex from biological material

Authors:

Garafutdinov R.R., Sakhabutdinova A.R., Aminev F.G., Chemeris A.V.

More about the authors

Journal: Forensic Medical Expertise. 2022;65(4): 36‑40

DOI: 10.17116/sudmed20226504136

Downloaded: 79

Download PDF (RU)

Contact the author

Table of contents

To cite this article:

Garafutdinov RR, Sakhabutdinova AR, Aminev FG, Chemeris AV. New polymorphic DNA marker to determine a person’s sex from biological material. Forensic Medical Expertise. 2022;65(4):36‑40. (In Russ.)
https://doi.org/10.17116/sudmed20226504136

Подписка 2025-2 (Forensic Medical Expertise)

Close metadata

The objective of the study was to pre-evaluate the applicability of gender-specific nucleotide sequences in human neuroligin genes as alternative DNA markers of sex. A new polymorphic locus based on NLGNX and NLGNY genes was proposed to establish the sex attribute of human biomaterials. The significant difference in the location of these loci relative to the pseudoautosomal region (PAR), as well as the combination of different types of polymorphism on the one hand, and the possibility of using gender-specific primers «in one assay» on the other hand, warrants their use as an additional marker of human sex attribute, including utilization as part of systems for DNA registration in the population. The introduction of a new polymorphic locus based on the NLGNX and NLGNY genes will make it possible to reliably identify the sex attribute of biological material recovered from crime scenes.

Keywords:

sex loci

sex attribute

neuroligin genes

forensic investigation

Authors:

Garafutdinov R.R.

Institute of Biochemistry and Genetics Ufa Federal Research Centre of the Russian Academy of Sciences

Sakhabutdinova A.R.

Institute of Biochemistry and Genetics Ufa Federal Research Centre of the Russian Academy of Sciences

Aminev F.G.

Institute of law, Bashkir State University

Chemeris A.V.

Kazakhstan Medical University of Continuing Education

Received:

14.12.2021

Accepted:

13.01.2022

List of references:

Tyler MG, Kirby LT, Wood S, Vernon S, Ferris JA. Human blood stain identification and sex determination in dried blood stains using recombinant DNA techniques. Forensic Science Int. 1986;31(4):267-272. https://doi.org/10.1016/0379-0738(86)90166-0
Nicklas JA, Buel E. Simultaneous determination of total human and male DNA using a real-time PCR assay. J Forensic Science. 2006;51(5):1005-1015. https://doi.org/10.1111/j.1556-4029.2006.00211.x
Page DC, Mosher R, Simpson EM, Fisher EM, Mardon G, Pollack J, de la Chapelle BMA, Brown LG. The sex-determining region of the human Y chromosome encodes a finger protein. Cell. 1987;51(6):1091-1104. https://doi.org/10.1016/0092-8674(87)90595-2
Mannucci A, Sullivan KM, Ivanov PL, Gill P. Forensic application of a rapid and quantitative DNA sex test by amplification of the X-Y homologous gene amelogenin. Int J Legal Med. 1994;106(4):190-193. PMID: 8038111. https://doi.org/10.1007/BF01371335
Nakahori Y, Takenaka O, Nakagome Y. A human X-Y homologous region encodes «amelogenin». Genomics. 1991;9(2):264-269. https://doi.org/10.1016/0888-7543(91)90251-9
Santos FR, Pandya A, Tyler-Smith C. Reliability of DNA-based sex tests. Nature Genetics. 1998;18(2):103. https://doi.org/10.1038/ng0298-103
Borovko S, Shyla A, Korban V, Borovko A. Amelogenin test abnormalities revealed in Belarusian population during forensic DNA analysis. Forensic Science Int: Genetics. 2015;15:98-104. https://doi.org/10.1016/j.fsigen.2014.10.014
Kashyap VK, Sahoo S, Sitalaximi T, Trivedi R. Deletions in the Y-derived amelogenin gene in the Indian population. BMC Medical Genetics. 2006;7:3. https://doi.org/10.1186/1471-2350-7-37
Sinclair AH, Berta P, Palmer MS, Hawkins JR, Griffiths BL, Smith MJ, Foster JW, Frischauf AM, Lovell-Badge R, Goodfellow PN. A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif. Nature. 1990;346(6281):240-244. https://doi.org/10.1038/346240a0
Acién P, Acién M. Disorders of Sex Development: Classification, Review, and Impact on Fertility. J Clin Med. 2020;9(11):3555. https://doi.org/10.3390/jcm9113555
Sambrook J, Russell DW. Isolation of DNA fragments from polyacrylamide gels by the crush and soak method. CSH Protocols. 2006. https://doi.org/10.1101/pdb.prot2936
Garafutdinov RR, Galimova AA, Sakhabutdinova AR, Vakhitov VA, Chemeris AV. PCR-based evaluation of sequence specificity of DNA fragmentation by ultrasound. Molekularnaya biologiya. 2016;50(2):272-278. (In Russ.).
Maxeiner S, Sester M, Krasteva-Christ G. Novel human sex-typing strategies based on the autism candidate gene NLGN4X and its male-specific gametologue NLGN4Y. Biology of Sex Differences. 2019;10(1):62. https://doi.org/10.1186/s13293-019-0279-x
Garafutdinov RR, Galimova AA, Sakhabutdinova AR. Polymerase chain reaction with nearby primers. Analytical Biochemistry. 2017;518:126-133. https://doi.org/10.1016/j.ab.2016.11.017
Garafutdinov RR, Galimova AA, Sakhabutdinova AR, Vakhitov VA, Chemeris AV. DNA amplification using PCR with abutting primers. Molekularnaya biologiya. 2015;49(4):628-637. (In Russ.).
Chemeris DA, Sagitov AM, Aminev FG, Lutsenko VI, Garafutdinov RR, Sakhabutdinova AR, Vasilov RG, Alekseev YaI, Slominsky PA, Khusnutdinova EK, Chemeris AV. Evolution of approaches to human DNA identification. Biomika. 2018;10(1):85-140. (In Russ.).
Garafutdinov RR, Sakhabutdinova AR, Alekseev YaI, Chemeris AV. Gender loci in DNA forensics and women’s sport. Biomika. 2021;13(1):54-74. (In Russ.).
Dedrickson K. Universal DNA databases: a way to improve privacy? J Law Biosciences. 2018;4(3):637-647. https://doi.org/10.1093/jlb/lsx041
Anisimov VA, Garafutdinov RR, Sagitov AM, Sakhabutdinova AR, Khusnutdinova EK, Aminev FG, Chemeris AV. DNA forensics: an origin, modern and prospects. Biomika. 2019;11(3):282-314. (In Russ.).
Chemeris DA, Garafutdinov RR, Sagitov AM, Sagitova MA, Mikhailenko KI, Zubov VV, Vasilov RG, Slominsky PA, Anisimov VA, Khusnutdinova EK, Alekseev YaI, Kurochkin VE, Lavrov GS, Aminev FG, Chemeris AV. Microdiplotypes as new markers for human DNA identification. Biomika. 2020;12(2):300-317. (In Russ.).
Aminev FG, Anisimov VA. On the organization of modern technology of universal DNA registration of citizens. Pravovoye gosudarstvo: teoriya i praktika. 2020;16(2):11-19. (In Russ.).
Chemeris AV, Anisimov VA, Aminev FG. Current issues of legal regulation of human genome databases creating and functioning. Pravovoye gosudarstvo: teoriya i praktika. 2020;16(2):134-144. (In Russ.).

Close metadata