Chemical and toxicological determination of lamivudine in biological substances

Illarionova E.A.; Chmelevskaya N.V.; Gonchikova Yu.A.

doi:https://doi.org/10.17116/sudmed20206301142

Illarionova E.A.

Irkutsk State Medical University, Ministry of Health of Russia, Irkutsk, Russia, 664003

Chmelevskaya N.V.

Forensic Chemical Department, Irkutsk Regional Bureau of Forensic Medicine, Irkutsk, Russia, 664022

Gonchikova Yu.A.

Irkutsk State Medical University, Ministry of Health of Russia, Irkutsk, Russia, 664003

Chemical and toxicological determination of lamivudine in biological substances

Authors:

Illarionova E.A., Chmelevskaya N.V., Gonchikova Yu.A.

More about the authors

Journal: Forensic Medical Expertise. 2020;63(1): 42‑46

DOI: 10.17116/sudmed20206301142

Read: 1785 times

Download PDF (RU)

Contact the author

Table of contents

To cite this article:

Illarionova EA, Chmelevskaya NV, Gonchikova YuA. Chemical and toxicological determination of lamivudine in biological substances. Forensic Medical Expertise. 2020;63(1):42‑46. (In Russ.)
https://doi.org/10.17116/sudmed20206301142

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

Использование инфографики авторами научных работ

Close metadata

Recommended articles:

Development of conditions for determination of imidazole derivatives in the blood using chromatography-mass spectrometry. Forensic Medical Expertise. 2025;(3):32-37

Abstract:

The purpose of the study is to develop a method for detecting, isolating and quantifying lamivudine in biological substances. Lamivudine was isolated by liquid-liquid and solid phase extraction. The conditions for isolating lamivudine (extractant, pH of the medium, electrolyte, time and frequency of extraction) from aqueous solutions were selected and methods were developed for isolating it from biological substances, including urine, saliva and liver, using liquid-liquid and solid phase extraction methods. Qualitative and quantitative analysis of lamivudine in extracts from urine, saliva and liver was performed by thin layer chromatography, UV spectrophotometry and high-performance liquid chromatography. A validation assessment of the developed techniques indicates their suitability for chemical and toxicological analysis of lamivudine.

Keywords:

lamivudine

liquid-liquid extraction

solid phase extraction

spectrophotometry

high-performance liquid chromatography

Authors:

Illarionova E.A.

Irkutsk State Medical University, Ministry of Health of Russia, Irkutsk, Russia, 664003

Chmelevskaya N.V.

Forensic Chemical Department, Irkutsk Regional Bureau of Forensic Medicine, Irkutsk, Russia, 664022

Gonchikova Yu.A.

Irkutsk State Medical University, Ministry of Health of Russia, Irkutsk, Russia, 664003

Close metadata

References:

Onishchenko GG. HIV infection: a challenge to humanity. VICH-infekciya i immunosupressii. 2009;1:5-9. (In Russ.)
Quercia R, Perno CF, Koteff J, Moore K, McCoig C, Clair MS, Kuritzkes D. Twenty-Five Years of Lamivudine: Current and Future Use for the Treatment of HIV-1 Infection. Journal of acquired immune deficiency syndromes. 2018;2(78):125-126. https://doi.org/10.1097/QAI.0000000000001660
Pokrovskij VV, Yurin OG, Kravchenko AV, Belyaeva VV, Kanestri VG, Ermak TN, Pokrovskaya AV. Treatment protocols for HIV-infected patients. Infekcionnye bolezni: Novosti. Mneniya. Obuchenie. 2012;1:56-71. (In Russ.)
Stepanova EV, Zakharova NG, Toropov SE, Minin PV. Side effects and optimization of highly active antiretroviral therapy according to the experience of Saint-Peterburg aids center. VICh-infekciya i immunosupressii. 2010;2:101-108. (In Russ.)
Gonchikova YuA, Chmelevskaya NV, Illarionova EA. Quantitation of lamivudine by UV spectrophotometry. Acta Biomedica Scientifica. 2017;4:90-92. (In Russ.)
Gonchikova YuA, Chmelevskaya NV, Illarionova EA. Development of a methodology for the quantitative determination of antiretroviral drugs using high-performance liquid chromatography. Medicinskij al’manah. 2018;2:98-100. (In Russ.)
Remezova IP, Lazaryan DS, Voronkov AV, Avramenko NS, Maksimenko TI, Sanzhieva D.Yu. The chemical toxicological analysis of sertindole. Sudebno-medicinskaya ekspertiza. 2016;1:35-39. (In Russ.) https://doi.org/10.17116/sudmed201659135-39
Golubev RS, Lyust EN, Malkova TL. The choice of the conditions for the preparation of the pregabalin samples from the biological fluids with the use of the liquid-liquid and solid phase extraction techniques. Sudebno-medicinskaya ekspertiza. 2018;3:40-43. (In Russ.) https://doi.org/10.17116/sudmed201861340-43
Vergejchik TH. Toksikologicheskaya khimiya: Uchebnoe posobie. M.: MEDpress-inform, 2012. (In Russ.)