The development and validation of the method for the identification of ethyl glucuronide and ethyl sulfate as the markers of the consumption of ethyl alcohol during one’s lifetime

Eroshchenko N.N.; Barsegian S.S.; Kiryushin A.N.; Tuaeva N.O.; Nosyrev A.E.; Salomatin V.E.

doi:https://doi.org/10.17116/sudmed201861442

Eroshchenko N.N.

Central Chemical Toxicological Laboratory of I.M. Sechenov First Moscow State Medical University, Russian Ministry of Health, Moscow, Russia, 119991

Barsegian S.S.

Biuro sudebno-meditsinskoĭ ékspertizy Departamenta zdravookhraneniia Moskvy

Kiryushin A.N.

Central Chemical Toxicological Laboratory of I.M. Sechenov First Moscow State Medical University, Russian Ministry of Health, Moscow, Russia, 119991

Tuaeva N.O.

Laboratory of Analytical Toxicology, V.P Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Ministry of Health of the Russia, Moscow, Russia, 119002

Nosyrev A.E.

Laboratory of Analytical Toxicology, V.P Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Ministry of Health of the Russia, Moscow, Russia, 119002;
Bureau of Forensic Medical Expertise, Moscow Health Department, Moscow, Russia, 115516

Salomatin V.E.

Russian Centre of Forensic Medical Expertise, Ministry of Health of the Russia, Moscow, Russia, 125284

The development and validation of the method for the identification of ethyl glucuronide and ethyl sulfate as the markers of the consumption of ethyl alcohol during one’s lifetime

Authors:

Eroshchenko N.N., Barsegian S.S., Kiryushin A.N., Tuaeva N.O., Nosyrev A.E., Salomatin V.E.

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Journal: Forensic Medical Expertise. 2018;61(4): 42‑47

DOI: 10.17116/sudmed201861442

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To cite this article:

Eroshchenko NN, Barsegian SS, Kiryushin AN, Tuaeva NO, Nosyrev AE, Salomatin VE. The development and validation of the method for the identification of ethyl glucuronide and ethyl sulfate as the markers of the consumption of ethyl alcohol during one’s lifetime. Forensic Medical Expertise. 2018;61(4):42‑47. (In Russ.)
https://doi.org/10.17116/sudmed201861442

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

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Abstract:

The objective of the present study was the development and validation of the rapid reproducible method for the identification of ethyl glucuronide and ethyl sulfate allowing to store and transport the study specimens without the loss of the substances of interest by placing the samples on the paper. We have developed the validated technique for the detection and quantitative determination of ethyl glucuronide and ethyl sulfate in the cadaveric blood and urine by means of low-resolution tandem mass-spectroscopy with the use of deuterated derivatives of these substances as the internal standards. The low threshold for quantitative determination of both above substances is 50 ng/ml for the blood and 100 ng/ml for the urine. The method is characterized by the accuracy and precision with the coefficient of variation below 15% and the influence of the matrix with the coefficient of variation below 15%. The evaluation of stability of the two analytes in blood when stored in the dry condition on the paper carrier during 2 weeks showed that the coefficient of variation did not exceed 6.4%. The comparative study of ethyl glucuronide and ethyl sulfate in the samples of cadaveric blood and urine containing from 0 to 5.2% of ethyl alcohol was carried out. The methods for the transportation of the biological fluids and for the extraction of ethyl glucuronide and ethyl sulfate placed on the paper carrier (Whatman 903) have been proposed. The possibility has been demonstrated to use ethyl glucuronide and ethyl sulfate as the markers of the consumption of ethyl alcohol during one’s lifetime for the purpose of investigation of the putrifactive changes of the blood components.

Keywords:

ethyl glucuronide

ethyl sulfate

ethyl alcohol

high performance liquid chromatography

mass-spectroscopy

Authors:

Eroshchenko N.N.

Central Chemical Toxicological Laboratory of I.M. Sechenov First Moscow State Medical University, Russian Ministry of Health, Moscow, Russia, 119991

Barsegian S.S.

Biuro sudebno-meditsinskoĭ ékspertizy Departamenta zdravookhraneniia Moskvy

Kiryushin A.N.

Central Chemical Toxicological Laboratory of I.M. Sechenov First Moscow State Medical University, Russian Ministry of Health, Moscow, Russia, 119991

Tuaeva N.O.

Laboratory of Analytical Toxicology, V.P Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Ministry of Health of the Russia, Moscow, Russia, 119002

Nosyrev A.E.

Salomatin V.E.

Russian Centre of Forensic Medical Expertise, Ministry of Health of the Russia, Moscow, Russia, 125284

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References:

Galitskii FA. Ekspertnaya otsenka obrazovaniya etanola v biologicheskikh ob’ektakh. M.: Akmola, 1997. (In Russ.)
O’Neal CL, Poklis A. Postmortem production of ethanol and factors that influence interpretation: a critical review. Am J Forensic Med Pathol. 1996;17(1):8-20. https://doi.org/10.1097/00000433-199603000-00002
Blackmore DJ. The bacterial production of ethyl alcohol. J Forensic Sci Soc. 1968;8:73-78. https://doi.org/10.1016/s0015-7368(68)70447-3
Lewis RJ, Johnson RD, Angier MK, Vu NT. Ethanol formation in unadulterated postmortem tissues. Forensic Sci Int. 2004;146:17-24. https://doi.org/10.1016/j.forsciint.2004.03.015
Jones AW, Hylen L, Svensson E, Helander A. Storage of specimens at 4 C or addition of sodium fluoride (1%) prevents formation of ethanol in urine inoculated with Candida albicans. J Anal Toxicol. 1999;23:333-336. https://doi.org/10.1093/jat/23.5.333
Corry JE. A review. Possible sources of ethanol ante- and post-mortem: its relationship to the biochemistry and microbiology of decomposition. J Appl Bacteriol. 1978;44:1-56. https://doi.org/10.1111/j.1365-2672.1978.tb00776.x
Baranowski S, Serr A, Thierauf A, Wemmann W, Grosse PM, Wurst FM, Hatter CC. In vitro study of bacterial degradation of ethyl glucoronide and ethyl sulphate. Int J Legal Med. 2008;122:389-393. https://doi.org/10.1007/s00414-008-0229-3
Helander A, Olsson I, Dahl H. Postcollection synthesis of ethyl glucuronide by bacteria in urine may cause false identification of alcohol consumption. Clin Chem. 2007;53:1855-1857. https://doi.org/10.1373/clinchem.2007.089482
Gorbacheva TV, Bushuev ES. To a question of storageability of ethanol in blood tests. Sudebnaya meditsina. 2016;2:104-105. (In Russ.)
Schmitt G, Aderjan R, Keller T, Wu M. Ethyl glucuronide: an unusual ethanol metabolite in humans. Synthesis, analytical data, and determination in serum and urine. J Anal Toxicol. 1995;19(2):91-94. https://doi.org/10.1093/jat/19.2.91
Skipper GE, Weinmann W, Thierauf A, Schaefer P, Wiesbeck G, Allen JP, Miller M, Wurst FM. Ethyl glucuronide: a biomarker to identify alcohol use by health professionals recovering from substance use disorders. Alcohol and Alcoholism. 2004;39(5):445-449. https://doi.org/10.1093/alcalc/agh078
Wurst FM, Skipper GE, Weinmann W. Ethyl glucuronide the direct ethanol metabolite on the threshold from science to routine use. Addiction. 2003;98(2):51-61. https://doi.org/10.1046/j.1359-6357.2003.00588.x
Salomatin EM, Barinskaya TO, Smirnov AV, Nikitin PV, Klyuev AE, Rumyantsev AD. Metodika vypolneniya izmerenii massovoi kontsentratsii etanola v krovi, moche i slyune. M.: FGBU «Rossiiskii tsentr sudebno-meditsinskoi ekspertizy» Minzdrava Rossii. M. 2012. (In Russ.)
Barsegyan SS, Salomatin EM, Pleteneva TV, Maksimova TV, Dolinkin AO. Metodicheskie rekomendatsii po validatsii analiticheskikh metodik, ispol’zuemykh v sudebno-khimicheskom i khimiko-toksikologicheskom analize biologicheskogo materiala. FGBU «Rossiiskii tsentr sudebno-meditsinskoi ekspertizy» Minzdrava Rossii. M. 2014. (In Russ.)
Guideline on Bioanalytical Method Validation. Eur Med Agency (London). 2015.
Rukovodstvo po jekspertize lekarstvennyh sredstv. M.: FGBU «NCJeSMP» Minzdrava Rossii. M. 2013. (In Russ.)
Vezzoli S, Bernini M, De Ferrari F. Ethyl glucuronide in vitreous humor and blood postmortem specimens: analysis by liquid chromatography-electrospray tandem mass spectrometry and interpreting results of neo-formation of ethanol. Ann Ist Super Sanita. 2015;51(1):19-27. https://doi.org/10.4415/ANN_15_01_05
Halter CC, Dresen S, Auwaerter V, Wurst FM, Weinmann W. Kinetics in serum and urinary excretion of ethyl sulfate and ethyl glucuronide after medium dose ethanol intake. International journal of legal medicin. 2008;122(2):123-128. https://doi.org/10.1007/s00414-007-0180-8