The peculiarities of determination of 2-chloro-1,4-dihydrooxybenzene in the biological material

Shormanov V.K.; Tsatsua E.P.; Koropova T.F.; Astashkina A.P.; Yangolenko G.G.

doi:https://doi.org/10.17116/sudmed201659544-50

Shormanov V.K.

Kurskiĭ gosudarstvennyĭ meditsinskiĭ universitet

Tsatsua E.P.

State budgetary educational institution of higher professional education "Kursk State Medical University", Russian Ministry of Health, Kursk, Russia, 305041

Koropova T.F.

Kursk State Medical University, Russian Ministry of Health, Kursk, Russia, 305041

Astashkina A.P.

GBOU VPO "Kurskiĭ gosudarstvennyĭ meditsinskiĭ universitet"

Yangolenko G.G.

Kursk State Medical University, Russian Ministry of Health, Kursk, Russia, 305041

The peculiarities of determination of 2-chloro-1,4-dihydrooxybenzene in the biological material

Authors:

Shormanov V.K., Tsatsua E.P., Koropova T.F., Astashkina A.P., Yangolenko G.G.

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Journal: Forensic Medical Expertise. 2016;59(5): 44‑50

DOI: 10.17116/sudmed201659544-50

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To cite this article:

Shormanov VK, Tsatsua EP, Koropova TF, Astashkina AP, Yangolenko GG. The peculiarities of determination of 2-chloro-1,4-dihydrooxybenzene in the biological material. Forensic Medical Expertise. 2016;59(5):44‑50. (In Russ.)
https://doi.org/10.17116/sudmed201659544-50

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

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Abstract:

The objective of the present study was to elucidate the peculiarities of the determination of 2-chloro-1,4-dihydrooxybenzene in the tissues of different organs and blood. The analytical methods used in the study included TLC, GC-MS, and UF-spectrophotometry. The study has demonstrated the usefulness of using acetone as an insulating solvent for the extraction of 2-chloro-1,4-dihydrooxybenzene from the biological materials. It was shown that the compound being analyzed can be separated from the endogenous substances contained in the biological matrices on the "Silasorb C-18" columns, 30 mcm. The method for the identification of 2-chloro-1,4-dihydrooxybenzene in the blood and organ tissues (such as normal and putrefied renal tissues) was developed. The method permits to determine 0.26 mg and 0.30 mg of the substance of interest in 100 g of the normal and putrefied renal tissues respectively; in addition, it measures 0.22 mg of 2-chloro-1,4-dihydrooxybenzene in the blood.

Keywords:

2-chloro-1,4-dihydrooxybenzene in the biological material

isolation

purification

identification and determination

Authors:

Shormanov V.K.

Kurskiĭ gosudarstvennyĭ meditsinskiĭ universitet

Tsatsua E.P.

State budgetary educational institution of higher professional education "Kursk State Medical University", Russian Ministry of Health, Kursk, Russia, 305041

Koropova T.F.

Kursk State Medical University, Russian Ministry of Health, Kursk, Russia, 305041

Astashkina A.P.

GBOU VPO "Kurskiĭ gosudarstvennyĭ meditsinskiĭ universitet"

Yangolenko G.G.

Kursk State Medical University, Russian Ministry of Health, Kursk, Russia, 305041

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