Isolation, extractive concentration, and determination of caffeine in the studies of blood plasma

Korenman Ia.I.; Shormanov V.K.; Mokshina N.Ia.; Krivosheeva O.A.; Golubitskiĭ G.B.

doi:https://doi.org/

Korenman Ia.I.

Voronezhskaia gosudarstvennaia tekhnologicheskaia akademiia

Shormanov V.K.

Kurskiĭ gosudarstvennyĭ meditsinskiĭ universitet

Mokshina N.Ia.

Voennyĭ aviatsionnyĭ inzhenernyĭ universitet, Voronezh

Krivosheeva O.A.

Voronezhskaia gosudarstvennaia tekhnologicheskaia akademiia

Golubitskiĭ G.B.

Kurskiĭ gosudarstvennyĭ meditsinskiĭ universitet

Isolation, extractive concentration, and determination of caffeine in the studies of blood plasma

Authors:

Korenman Ia.I., Shormanov V.K., Mokshina N.Ia., Krivosheeva O.A., Golubitskiĭ G.B.

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Journal: Forensic Medical Expertise. 2012;55(2): 32‑35

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Korenman IaI, Shormanov VK, Mokshina NIa, Krivosheeva OA, Golubitskiĭ GB. Isolation, extractive concentration, and determination of caffeine in the studies of blood plasma. Forensic Medical Expertise. 2012;55(2):32‑35. (In Russ.)

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

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Abstract:

The optimal conditions for the isolation of caffeine from human blood by means of acetone extraction are described with special reference to the peculiarities of extraction from aqueous solutions. The possibility of concentration and purification of caffeine from blood plasma using acetone and aceton-chlorophorm mixture (2:8) as the solvents is illustrated. In addition, purification by silica-gel thin layer chromatography is discussed. Thin layer chromatography, UV-spectrophotometry, and high performance liquid chromatography are considered as potential methods for the identification and quantitative determination of caffeine.

Keywords:

caffeine

isolation

purification

extractive concentration

identification and quantitation

Authors:

Korenman Ia.I.

Voronezhskaia gosudarstvennaia tekhnologicheskaia akademiia

Shormanov V.K.

Kurskiĭ gosudarstvennyĭ meditsinskiĭ universitet

Mokshina N.Ia.

Voennyĭ aviatsionnyĭ inzhenernyĭ universitet, Voronezh

Krivosheeva O.A.

Voronezhskaia gosudarstvennaia tekhnologicheskaia akademiia

Golubitskiĭ G.B.

Kurskiĭ gosudarstvennyĭ meditsinskiĭ universitet

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